LOCUS       sequence-249125-        3976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic Part T- Terminator;  L2U2H09; synthetic terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 13 to 47)
  AUTHORS   Valenzuela-Ortega M, French C
  TITLE     Joint universal modular plasmids (JUMP): a flexible vector platform 
            for synthetic biology.
  JOURNAL   Synth Biol (Oxf). 2021 Feb 2;6(1):ysab003. doi: 
            10.1093/synbio/ysab003. eCollection 2021.
  PUBMED    33623824
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2021-02-2"; volume: "6(1):ysab003. doi"; pages: " 
            10.1093/synbio/ysab003. eCollection 2021"
COMMENT     Sequence Label: pJUMP19-L2U2H09_T
FEATURES             Location/Qualifiers
     source          1..3976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        13..47
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     misc_feature    101..122
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    209..229
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     RBS             257..277
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             297..1004
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     CDS             297..1004
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNF
                     KIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
                     TAAGITHGMDELYK"
     primer_bind     complement(321..349)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     944..964
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1044..1138
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1211..1302
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1303..1371,1372..2163)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      2167..2206
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            2353..2461
                     /note="incP origin of transfer"
     rep_origin      2497..3085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2986..3005
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3239..3256
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3271..3411
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3337..3356)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3497..3519
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3513..3704)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     terminator      complement(3884..3970)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaagg agttgacggc tagctcagtc ctaggtacag tgctagcgta cttaacttct
       61 gactgagttg cacgcttaac ttctgactga gttgcacgct gaattcgcgg ccgcttctag
      121 agggtctgcg atgtttggtc tctgctttcg aacggccctc gcaagggccg tttttttgta
      181 tgttcgcttg agacctttca tcgcgaccta ctagtagcgg ccgctgcagg gagtaacttc
      241 tgactgagtt gcatacttgt ttaactttaa gaaggagata tacaatggta gcccgtaaag
      301 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
      361 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
      421 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
      481 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt
      541 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
      601 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
      661 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
      721 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
      781 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
      841 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
      901 aatctgccct ttcgaaagat cccaacgaaa agagagatca catggtcctt cttgagtttg
      961 taacagctgc tgggattaca catggcatgg atgaactata caaaggttcg taacttctga
     1021 ctgagttgca cgctctagtc ttggactcct gttgatagat ccagtaatga cctcagaact
     1081 ccatctggat ttgttcagaa cgctcggttg ccgccgggcg ttttttattg gtgagaatcc
     1141 aggggtcccc aataattacg atttaaatta gtagcccgcc taatgagcgg gctttttttt
     1201 aattccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1261 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagcat tcagcatttt
     1321 cgtgtggcgc tgattccgtt ttttgcggcg ttttgcctgc cggtgtttgc gcatccggaa
     1381 accctggtga aagtgaaaga tgcggaagat caactgggtg cgcgcgtggg ctatattgaa
     1441 ctggatctga acagcggcaa aattctggaa tcttttcgtc cggaagaacg ttttccgatg
     1501 atgagcacct ttaaagtgct gctgtgcggt gcggttctga gccgtgtgga tgcgggccag
     1561 gaacaactgg gccgtcgtat tcattatagc cagaacgatc tggtggaata tagcccggtg
     1621 accgaaaaac atctgaccga tggcatgacc gtgcgtgaac tgtgcagcgc ggcgattacc
     1681 atgagcgata acaccgcggc gaacctgctg ctgacgacca ttggcggtcc gaaagaactg
     1741 accgcgtttc tgcataacat gggcgatcat gtgacccgtc tggatcgttg ggaaccggaa
     1801 ctgaacgaag cgattccgaa cgatgaacgt gataccacca tgccggcagc aatggcgacc
     1861 accctgcgta aactgctgac gggtgagctg ctgaccctgg caagccgcca gcaactgatt
     1921 gattggatgg aagcggataa agtggcgggt ccgctgctgc gtagcgcgct gccggctggc
     1981 tggtttattg cggataaaag cggtgcgggc gaacgtggca gccgtggcat tattgcggcg
     2041 ctgggcccgg atggtaaacc gagccgtatt gtggtgattt ataccaccgg cagccaggcg
     2101 acgatggatg aacgtaaccg tcagattgcg gaaattggcg cgagcctgat taaacattgg
     2161 taaaccgata caattaaagg ctccttttgg agcctttttt tttggacgac ccttgtcctt
     2221 ttccgctgca taaccctgct tcggggtcat tatagcgatt ttttcggtat atccatcctt
     2281 tttcgcacga tatacaggat tttgccaaag ggttcgtgta gactttcctt ggtgtatcca
     2341 acggcgtcag ccgggcagga taggtgaagt aggcccaccc gcgagcgggt gttccttctt
     2401 cactgtccct tattcgcacc tggcggtgct caacgggaat cctgctctgc gaggctggcc
     2461 gtaggccggc cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2521 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2581 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2641 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2701 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2761 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2821 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2881 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2941 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3001 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3061 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3121 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3181 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3241 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     3301 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     3361 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     3421 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     3481 caagctgtga cagtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     3541 cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gcagcgattc acagatgtct
     3601 gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg
     3661 ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa
     3721 gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata
     3781 cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg
     3841 gtatggatgc ggcgggggcg cgcccagctg tctagggcgg cggatttgtc ctactcagga
     3901 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc
     3961 gttttatttg atgcct
//
