LOCUS       sequence-249157-        9413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  sgRNA expression vector compatible with CROP-Seq and imaging 
            screens.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Tian R, Gachechiladze MA, Ludwig CH, Laurie MT, Hong JY, Nathaniel 
            D, Prabhu AV, Fernandopulle MS, Patel R, Abshari M, Ward ME, 
            Kampmann M
  TITLE     CRISPR Interference-Based Platform for Multimodal Genetic Screens in
            Human iPSC-Derived Neurons.
  JOURNAL   Neuron. 2019 Aug 5. pii: S0896-6273(19)30640-3. doi: 
            10.1016/j.neuron.2019.07.014.
  PUBMED    31422865
REFERENCE   2  (bases 1 to 9413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2019 Aug 5. pii: S0896-6273(19)30640-3. doi: 
            10.1016/j.neuron.2019.07.014."
COMMENT     Sequence Label: pMK1334
FEATURES             Location/Qualifiers
     source          1..9413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        136..1314
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          367..1305
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1262..1282
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1327..1923
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1327..1346)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1823..1843
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1933..1986
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1993..2019
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             2035..2061
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     regulatory      2077..2086
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2083..2793
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     protein_bind    complement(2816..2849)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2905..3493
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2958..2978)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3376..3387)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3841..3874)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     3878..3898
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     LTR             4120..4300
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    4401..4522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4438..4457)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4492..4511
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4562..4697
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4717..4736)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4718..4736)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4746..4763)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4746..4762)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4755..4777)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4904..5359
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4991..5010)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5201..5222
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5385..5489
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5490..5558,5559..6350)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5708..5727)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6521..7109
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7010..7029
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7263..7280
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7397..7418
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7433..7438,7439..7456,7457..7463)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7471..7487
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7476..7498
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7495..7511
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7495..7511
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7530..7550
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7532..7550
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7578..7804
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7805..7985
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8032..8157
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8650..8883
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9068..9112
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9293..9410
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtttattaca gggacagcag agatccactt tggcgccggc tcgagggggc ccgggtgcaa
       61 agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg
      121 aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
      181 cccgagaagt tggggggagg ggtcggcaat tgatccggtg cctagagaag gtggcgcggg
      241 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
      301 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
      361 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
      421 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
      481 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
      541 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
      601 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
      661 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
      721 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
      781 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
      841 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
      901 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
      961 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     1021 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     1081 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     1141 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     1201 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     1261 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgacgtacg
     1321 gccaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     1381 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     1441 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     1501 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg
     1561 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     1621 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     1681 aaggagcccg cgtggttcct ggccaccgtc ggagtctcgc ccgaccacca gggcaagggt
     1741 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     1801 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     1861 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     1921 gccggatcgg gagagggcag aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct
     1981 ggcccaccgg tccctgccgc taaacgggtc aaactcgacg gcggttctgg aggtcctgct
     2041 gcaaagcgcg ttaagttgga tggagggtca gggggagcca ccatggtgtc taagggcgaa
     2101 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat
     2161 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
     2221 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc
     2281 ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag
     2341 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg
     2401 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
     2461 ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
     2521 ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg
     2581 aagctcgtgg gcgggagcca tctgatcgca aacgccaaga ccacatatag atccaagaaa
     2641 cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc
     2701 aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc
     2761 gacctcccta gcaaactggg gcacaagctt aattaagaat tcgtcgaggg acctaataac
     2821 ttcgtatagc atacattata cgaagttata catgtttaag ggttccggtt ccactaggta
     2881 caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     2941 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3001 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3061 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3121 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3181 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3241 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     3301 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3361 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3421 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3481 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     3541 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatt
     3601 tcaccattat cgtttcagac ccacctccca accccgaggg gaccccgatc cgacgcgcca
     3661 tctctaggcc cgcgccggcc ccctcgcacg gacttgtggg agaagctcgg ctactcccct
     3721 gccccggtta atttgcatat aatatttcct agtaactata gaggcttaat gtgcgataaa
     3781 agacagataa tctgttcttt ttaatactag ctacatttta catgataggc ttggatttct
     3841 ataacttcgt atagcataca ttatacgaag ttataaacag cacaaaagga aactcaccct
     3901 aactgtaaag taattgtgtg ttttgagact ataagtatcc cttggagaac caccttgttg
     3961 gaccaggatg ggcaccaccc gtttaagagc taagctggaa acagcatagc aagtttaaat
     4021 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttaagcttg
     4081 gcgtaactag atcttgagac actgcttttt gcttgtactg ggtctctctg gttagaccag
     4141 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     4201 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     4261 tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt
     4321 catcttagta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     4381 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     4441 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     4501 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     4561 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4621 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     4681 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     4741 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     4801 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     4861 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     4921 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4981 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5041 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5101 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5161 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5221 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5281 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     5341 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5401 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5461 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5521 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5581 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5641 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5701 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5761 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5821 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5881 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     5941 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6001 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6061 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6121 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6181 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6241 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6301 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6361 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6421 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6481 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6541 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6601 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6661 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6721 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6781 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6841 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6901 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6961 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7021 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7081 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7141 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7201 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7261 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7321 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     7381 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     7441 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     7501 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     7561 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     7621 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     7681 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     7741 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     7801 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7861 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7921 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7981 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     8041 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     8101 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     8161 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     8221 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     8281 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     8341 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     8401 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     8461 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     8521 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     8581 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     8641 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     8701 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     8761 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     8821 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     8881 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     8941 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     9001 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     9061 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     9121 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     9181 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     9241 ttcaccatta tcgtttcaga atcttgagac aaatggcagt attcatccac aattttaaaa
     9301 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     9361 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgg
//
