LOCUS       sequence-249159-       10347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10347)
  AUTHORS   Dans M, Gagnoux-Palacios L, Blaikie P, Klein S, Mariotti A, 
            Giancotti FG
  TITLE     Tyrosine phosphorylation of the beta 4 integrin cytoplasmic domain 
            mediates Shc signaling to extracellular signal-regulated kinase and 
            antagonizes formation of hemidesmosomes.
  JOURNAL   J Biol Chem. 2001 Jan 12. 276(2):1494-502.
  PUBMED    11044453
REFERENCE   2  (bases 1 to 10347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2001 Jan 12. 276(2):1494-502."
COMMENT     Sequence Label: pRK5 beta4
FEATURES             Location/Qualifiers
     source          1..10347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      6647..6656
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6660..6794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6697..6716)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6751..6770
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        6863..7220
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6863..7054
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6886..6906)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7071..7206
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7240..7257)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7240..7256)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7249..7271)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7469..7924
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7556..7575)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7766..7787
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7941..7960)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8060..8082
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8120..8138)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8206..8310
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8311..8379,8380..9171)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8529..8548)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9342..9930
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9831..9850
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10084..10101
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10218..10239
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10254..10259,10260..10277,10278..10284)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10292..10308
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10297..10319
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10316..10332
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10316..10332
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attcggcgcc cgcgcgctgc agccccatct cctagcggca
      961 gcccaggcgc ggagggagcg agtccgcccc gaggtaggtc caggacgggc gcacagcagc
     1021 agccgaggct ggccgggaga gggaggaaga ggatggcagg gccacgcccc agcccatggg
     1081 ccaggctgct cctggcagcc ttgatcagcg tcagcctctc tgggaccttg gcaaaccgct
     1141 gcaagaaggc cccagtgaag agctgcacgg agtgtgtccg tgtggataag gactgcgcct
     1201 actgcacaga cgagatgttc agggaccggc gctgcaacac ccaggcggag ctgctggccg
     1261 cgggctgcca gcgggagagc atcgtggtca tggagagcag cttccaaatc acagaggaga
     1321 cccagattga caccaccctg cggcgcagcc agatgtcccc ccaaggcctg cgggtccgtc
     1381 tgcggcccgg tgaggagcgg cattttgagc tggaggtgtt tgagccactg gagagccccg
     1441 tggacctgta catcctcatg gacttctcca actccatgtc cgatgatctg gacaacctca
     1501 agaagatggg gcagaacctg gctcgggtcc tgagccagct caccagcgac tacactattg
     1561 gatttggcaa gtttgtggac aaagtcagcg tcccgcagac ggacatgagg cctgagaagc
     1621 tgaaggagcc ctggcccaac agtgaccccc ccttctcctt caagaacgtc atcagcctga
     1681 cagaagatgt ggatgagttc cggaataaac tgcagggaga gcggatctca ggcaacctgg
     1741 atgctcctga gggcggcttc gatgccatcc tgcagacagc tgtgtgcacg agggacattg
     1801 gctggcgccc ggacagcacc cacctgctgg tcttctccac cgagtcagcc ttccactatg
     1861 aggctgatgg cgccaacgtg ctggctggca tcatgagccg caacgatgaa cggtgccacc
     1921 tggacaccac gggcacctac acccagtaca ggacacagga ctacccgtcg gtgcccaccc
     1981 tggtgcgcct gctcgccaag cacaacatca tccccatctt tgctgtcacc aactactcct
     2041 atagctacta cgagaagctt cacacctatt tccctgtctc ctcactgggg gtgctgcagg
     2101 aggactcgtc caacatcgtg gagctgctgg aggaggcctt caatcggatc cgctccaacc
     2161 tggacatccg ggccctagac agcccccgag gccttcggac agaggtcacc tccaagatgt
     2221 tccagaagac gaggactggg tcctttcaca tccggcgggg ggaagtgggt atataccagg
     2281 tgcagctgcg ggcccttgag cacgtggatg ggacgcacgt gtgccagctg ccggaggacc
     2341 agaagggcaa catccatctg aaaccttcct tctccgacgg cctcaagatg gacgcgggca
     2401 tcatctgtga tgtgtgcacc tgcgagctgc aaaaagaggt gcggtcagct cgctgcagct
     2461 tcaacggaga cttcgtgtgc ggacagtgtg tgtgcagcga gggctggagt ggccagacct
     2521 gcaactgctc caccggctct ctgagtgaca ttcagccctg cctgcgggag ggcgaggaca
     2581 agccgtgctc cggccgtggg gagtgccagt gcgggcactg tgtgtgctac ggcgaaggcc
     2641 gctacgaggg tcagttctgc gagtatgaca acttccagtg tccccgcact tccgggttcc
     2701 tctgcaatga ccgaggacgc tgctccatgg gccagtgtgt gtgtgagcct ggttggacag
     2761 gcccaagctg tgactgtccc ctcagcaatg ccacctgcat cgacagcaat gggggcatct
     2821 gtaatggacg tggccactgt gagtgtggcc gctgccactg ccaccagcag tcgctctaca
     2881 cggacaccat ctgcgagatc aactactcgg cgatccaccc gggcctctgc gaggacctac
     2941 gctcctgcgt gcagtgccag gcgtggggca ccggcgagaa gaaggggcgc acgtgtgagg
     3001 aatgcaactt caaggtcaag atggtggacg agcttaagag agccgaggag gtggtggtgc
     3061 gctgctcctt ccgggacgag gatgacgact gcacctacag ctacaccatg gaaggtgacg
     3121 gcgcccctgg gcccaacagc actgtcctgg tgcacaagaa gaaggactgc cctccgggct
     3181 ccttctggtg gctcatcccc ctgctcctcc tcctcctgcc gctcctggcc ctgctactgc
     3241 tgctatgctg gaagtactgt gcctgctgca aggcctgcct ggcacttctc ccgtgctgca
     3301 accgaggtca catggtgggc tttaaggaag accactacat gctgcgggag aacctgatgg
     3361 cctctgacca cttggacacg cccatgctgc gcagcgggaa cctcaagggc cgtgacgtgg
     3421 tccgctggaa ggtcaccaac aacatgcagc ggcctggctt tgccactcat gccgccagca
     3481 tcaaccccac agagctggtg ccctacgggc tgtccttgcg cctggcccgc ctttgcaccg
     3541 agaacctgct gaagcctgac actcgggagt gcgcccagct gcgccaggag gtggaggaga
     3601 acctgaacga ggtctacagg cagatctccg gtgtacacaa gctccagcag accaagttcc
     3661 ggcagcagcc caatgccggg aaaaagcaag accacaccat tgtggacaca gtgctgatgg
     3721 cgccccgctc ggccaagccg gccctgctga agcttacaga gaagcaggtg gaacagaggg
     3781 ccttccacga cctcaaggtg gcccccggct actacaccct cactgcagac caggacgccc
     3841 ggggcatggt ggagttccag gagggcgtgg agctggtgga cgtacgggtg cccctcttta
     3901 tccggcctga ggatgacgac gagaagcagc tgctggtgga ggccatcgac gtgcccgcag
     3961 gcactgccac cctcggccgc cgcctggtaa acatcaccat catcaaggag caagccagag
     4021 acgtggtgtc ctttgagcag cctgagttct cggtcagccg cggggaccag gtggcccgca
     4081 tccctgtcat ccggcgtgtc ctggacggcg ggaagtccca ggtctcctac cgcacacagg
     4141 atggcaccgc gcagggcaac cgggactaca tccccgtgga gggtgagctg ctgttccagc
     4201 ctggggaggc ctggaaagag ctgcaggtga agctcctgga gctgcaagaa gttgactccc
     4261 tcctgcgggg ccgccaggtc cgccgtttcc acgtccagct cagcaaccct aagtttgggg
     4321 cccacctggg ccagccccac tccaccacca tcatcatcag ggacccagat gaactggacc
     4381 ggagcttcac gagtcagatg ttgtcatcac agccaccccc tcacggcgac ctgggcgccc
     4441 cgcagaaccc caatgctaag gccgctgggt ccaggaagat ccatttcaac tggctgcccc
     4501 cttctggcaa gccaatgggg tacagggtaa agtactggat tcagggtgac tccgaatccg
     4561 aagcccacct gctcgacagc aaggtgccct cagtggagct caccaacctg tacccgtatt
     4621 gcgactatga gatgaaggtg tgcgcctacg gggctcaggg cgagggaccc tacagctccc
     4681 tggtgtcctg ccgcacccac caggaagtgc ccagcgagcc agggcgtctg gccttcaatg
     4741 tcgtctcctc cacggtgacc cagctgagct gggctgagcc ggctgagacc aacggtgaga
     4801 tcacagccta cgaggtctgc tatggcctgg tcaacgatga caaccgacct attgggccca
     4861 tgaagaaagt gctggttgac aaccctaaga accggatgct gcttattgag aaccttcggg
     4921 agtcccagcc ctaccgctac acggtgaagg cgcgcaacgg ggccggctgg gggcctgagc
     4981 gggaggccat catcaacctg gccacccagc ccaagaggcc catgtccatc cccatcatcc
     5041 ctgacatccc tatcgtggac gcccagagcg gggaggacta cgacagcttc cttatgtaca
     5101 gcgatgacgt tctacgctct ccatcgggca gccagaggcc cagcgtctcc gatgacactg
     5161 agcacctggt gaatggccgg atggactttg ccttcccggg cagcaccaac tccctgcaca
     5221 ggatgaccac gaccagtgct gctgcctatg gcacccacct gagcccacac gtgccccacc
     5281 gcgtgctaag cacatcctcc accctcacac gggactacaa ctcactgacc cgctcagaac
     5341 actcacactc gaccacactg ccgagggact actccaccct cacctccgtc tcctcccacg
     5401 actctcgcct gactgctggt gtgcccgaca cgcccacccg cctggtgttc tctgccctgg
     5461 ggcccacatc tctcagagtg agctggcagg agccgcggtg cgagcggccg ctgcagggct
     5521 acagtgtgga gtaccagctg ctgaacggcg gtgagctgca tcggctcaac atccccaacc
     5581 ctgcccagac ctcggtggtg gtggaagacc tcctgcccaa ccactcctac gtgttccgcg
     5641 tgcgggccca gagccaggaa ggctggggcc gagagcgtga gggtgtcatc accattgaat
     5701 cccaggtgca cccgcagagc ccactgtgtc ccctgccagg ctccgccttc actttgagca
     5761 ctcccagtgc cccaggcccg ctggtgttca ctgccctgag cccagactcg ctgcagctga
     5821 gctgggagcg gccacggagg cccaatgggg atatcgtcgg ctacctggtg acctgtgaga
     5881 tggcccaagg aggagggcca gccaccgcat tccgggtgga tggagacagc cccgagagcc
     5941 ggctgaccgt gccgggcctc agcgagaacg tgccctacaa gttcaaggtg caggccagga
     6001 ccactgaggg cttcgggcca gagcgcgagg gcatcatcac catagagtcc caggatggag
     6061 gacccttccc gcagctgggc agccgtgccg ggctcttcca gcacccgctg caaagcgagt
     6121 acagcagcat caccaccacc cacaccagcg ccaccgagcc cttcctagtg gatgggccga
     6181 ccctgggggc ccagcacctg gaggcaggcg gctccctcac ccggcatgtg acccaggagt
     6241 ttgtgagccg gacactgacc accagcggaa cccttagcac ccacatggac caacagttct
     6301 tccaaacttg accgcaccct gccccacccc cgccatgtcc cactaggcgt cctcccgact
     6361 cctctcccgg agcctcctca gctactccat ccttgcaccc ctgggggccc agcccacccg
     6421 catgcacaga gcaggggcta ggtgtctcct gggaggcatg aagggggcaa ggtccgtcct
     6481 ctgtgggccc aaacctattt gtaaccaaag agctgggagc agcacaagga cccagccttt
     6541 gttctgcact taataaatgg ttttgctact gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     6601 aaccgaattc cccggggatc ctctagagtc gacctgcaga agcttggccg ccatggccca
     6661 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6721 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6781 atcatgtctg gatcgggaat taattcggcg cagcaccatg gcctgaaata acctctgaaa
     6841 gaggaacttg gttaggtacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
     6901 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6961 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7021 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     7081 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     7141 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     7201 gaggcctagg cttttgcaaa aagctgttaa cagcttggca ctggccgtcg ttttacaacg
     7261 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     7321 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     7381 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     7441 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     7501 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     7561 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7621 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7681 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     7741 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7801 cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     7861 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     7921 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     7981 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     8041 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     8101 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     8161 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     8221 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     8281 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     8341 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     8401 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     8461 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     8521 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     8581 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     8641 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     8701 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     8761 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     8821 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     8881 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     8941 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     9001 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     9061 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     9121 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     9181 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     9241 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     9301 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     9361 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9421 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     9481 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     9541 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     9601 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     9661 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     9721 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9781 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9841 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9901 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     9961 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    10021 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    10081 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
    10141 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
    10201 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
    10261 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
    10321 aacagctatg acatgattac gaattaa
//
