LOCUS       sequence-249171-        9688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac transposon vector encoding the receptor MOR170-1 and the 
            barcoded CRE reporter gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9688)
  AUTHORS   Jones EM, Jajoo R, Cancilla D, Lubock NB, Wang J, Satyadi M, Cheung 
            R, de March C, Bloom JS, Matsunami H, Kosuri S
  TITLE     A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions 
            with RNA Sequencing.
  JOURNAL   Cell Syst. 2019 Mar 27;8(3):254-260.e6. doi: 
            10.1016/j.cels.2019.02.009. Epub 2019 Mar 20.
  PUBMED    30904378
REFERENCE   2  (bases 1 to 9688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2019.02.009"; journalName: "Cell Syst"; date: 
            "2019-03-27- 27"; volume: "8"; issue: "3"; pages: "254-260.e6"
COMMENT     Sequence Label: pB-MultiplexedReporter-MOR170-1
FEATURES             Location/Qualifiers
     source          1..9688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(53..844,845..913))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     676..695
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(914..1018)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1044..1499)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1181..1202)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1393..1412
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1626..1648
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1640..1657
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1641..1657
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1787..1849
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2595..2626
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     gap             3370..3384
                     /estimated_length=15
     polyA_signal    3817..3951
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    4049..4067
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4086..4104
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4123..4141
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4160..4178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4197..4215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4234..4252
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4271..4289
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4308..4326
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    5707..5828
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6073..6284
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6297..6565
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     primer_bind     complement(7109..7129)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    7171..7292
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   8055..8089
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8580..8596)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8580..8596)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8593..8615)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8604..8620
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8628..8634,8635..8652,8653..8658))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8673..8694
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8811..8828)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8982..9570)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9062..9081)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
       61 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
      121 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
      181 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
      241 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
      301 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
      361 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
      421 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
      481 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
      541 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
      601 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
      661 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
      721 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
      781 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
      841 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
      901 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
      961 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     1021 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     1081 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1141 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     1201 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1261 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1321 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1381 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1441 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     1501 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     1561 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     1621 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcctcgt tcattcacgt
     1681 ttttgaaccc gtggaggacg ggcagactcg cggtgcaaat gtgttttaca gcgtgatgga
     1741 gcagatgaag atgctcgaca cgctgcagaa cacgcagcta gattaaccct agaaagataa
     1801 tcatattgtg acgtacgtta aagataatca tgtgtaaaat tgacgcatgt gttttatcgg
     1861 tctgtatatc gaggtttatt tattaatttg aatagatatt aagttttatt atatttacac
     1921 ttacatacta ataataaatt caacaaacaa tttatttatg tttatttatt tattaaaaaa
     1981 aacaaaaact caaaatttct tctataaagt aacaaaactt ttatgaggga cagccccccc
     2041 ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc
     2101 cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac
     2161 agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct
     2221 ttgagcctgc agacacctgg ggggatacgg ggaaaaggcc tccacggcca ctagtattat
     2281 gcccagtaca tgaccttatg aaccgtcgtg acgtcagaca gaccacgcga tcgctcgagt
     2341 ccgccggtca atccggtgac gtcacgggcc tcttcgctat tacgccagct ggcgaaaggg
     2401 ggttgacgtc acattaaatc ggccaacgcg cggggagagg cggtgacgtc aacaggcatc
     2461 gtggtgtcac gctcgtcgtg acgtcagtcg ctttaactgg ccctggcttt ggcagcctgt
     2521 gctcagcctg acgtcagagg cctgacgtca gagagcctga cgtcagagag cctgacgtca
     2581 gagagcggag actctagagg gtatataatg gaagctcgaa ttccagcttg gcattccggt
     2641 actgttggta aaatggtgtc caagggcgaa gaggacaaca tggccatcat caaagaattc
     2701 atgcggttca aggtgcacat ggaaggcagc gtgaacggcc acgagttcga gattgagggc
     2761 gagggcgaag gcagacccta cgagggaaca cagaccgcca agctgaaagt gaccaagggc
     2821 ggacccctgc ccttcgcctg ggatatcctg agcccccagt ttatgtacgg cagcaaggcc
     2881 tacgtgaagc accccgccga catccccgac tacctgaagc tgagcttccc cgagggcttc
     2941 aagtgggaga gagtgatgaa cttcgaggac ggcggcgtcg tgaccgtgac ccaggatagc
     3001 tctctgcagg acggcgagtt catctacaaa gtgaagctgc ggggcaccaa cttccccagc
     3061 gacggacccg tgatgcagaa aaagaccatg ggctgggagg ccagcagcga gagaatgtac
     3121 cctgaggatg gcgccctgaa gggcgagatc aagcagcggc tgaaactgaa ggatggcggc
     3181 cactacgacg ccgaagtgaa aaccacctac aaggccaaga aacccgtgca gctgcctggc
     3241 gcctacaacg tgaacatcaa gctggacatc accagccaca acgaggacta caccatcgtg
     3301 gaacagtacg agcgggccga gggcagacac tctacaggcg gaatggacga gctgtacaag
     3361 tgaacgcgtn nnnnnnnnnn nnnncgacgc atctgattaa agggggtacc ttatgttcac
     3421 aactggcgtg aatgtaactg tattcagcga tgacccacac aggcatagag tgtctgctat
     3481 taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
     3541 ggaatatttg atgtatagtg ccttgactag aaccggtgcg atgacacact agtccacaca
     3601 ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa
     3661 tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc
     3721 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     3781 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     3841 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     3901 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccccaggaag
     3961 ctcctctgtg tcctcataaa ccctaacctc ggatccgagg acattccaat cataggggta
     4021 cccgacccat caagtgccac ctgacgtctc cctatcagtg atagagaagt cgacacgtct
     4081 cgagctccct atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag
     4141 aagtcgacac gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc
     4201 tatcagtgat agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtacgt
     4261 ctagaacgtc tccctatcag tgatagagaa gtcgacacgt ctcgagctcc ctatcagtga
     4321 tagagaaggt accccctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
     4381 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctgagctc
     4441 gtttagtgac cgtcagatca ctagaagctt tattgcggta gtttatcaca gttaaattgc
     4501 taacgcagtc agtgcttctg gccgccacca tgagacccca gatcctgctg ctcctggccc
     4561 tgctgaccct aggcctggct catatgaatg gcacagaagg ccctaacttc tacgtgccct
     4621 tctccaatgc gacgggtgtg gtacgcgaat tcacgcgtat gccactcaat gcacaaaaga
     4681 ctatggaaaa tgattcttct gtgtctgagt ttattcttat gggactgaca gaccaacctg
     4741 agctccagct gcccttattt gttctgttct tggtgaacta cacagtcact gtgatgggaa
     4801 atttgagctt aatgaatctc atttgcctaa actcaaacct tcacaccccc atgtactttt
     4861 tcatcttcaa cctgtccttc attgacttct gttattcaat ggtctttacc cccaaaatgc
     4921 tgatgggctt tgttgtagag aagaacatca tctccttcag aggatgcatg actcagctgt
     4981 ttttcttcct attttttgtg aactctgaga gttatgtgct gacagcaatg gcctatgatc
     5041 gctatgtggc catctgtcag ccactgctct acaaagctgt tatgtcacct ggcatctgct
     5101 ttctattgat attttgtact tatttgatgg ggcttgttag tgccttattc catacaggtt
     5161 ttatgataag gctcaacttt tgtgattcta atgttatcaa ccactatatg tgtgatatct
     5221 tccctctctt tcggctctcc tgcagtagca cctatctcac tgagcttgtg agctctgctg
     5281 tggtgggtac agctattatc ttatgttgcc tcattatctt aatctcttat ggtatgatcc
     5341 tttacaatat cattcatatg tcatcaggta agggttggtc caaagccttg ggcacttgtg
     5401 gttctcacat tataactgtt agcttgttct atgtaactgg gatgcttgct tatgtcaagc
     5461 catcatctgc tgagactgtg ggacagggga aaattttctc agtattttac acttttttgg
     5521 tgcccatgct gaatcctctt atttacagcc tcaggaacaa ggatgtcaaa cttgctgtga
     5581 aaaaaacctg gaagagactc acatgttaag cggccgcttc gagcagacat gataagacct
     5641 gcaggtggaa agactcctca gcagggccga ctcttgtcca tgagcggccg cttcgagcag
     5701 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     5761 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     5821 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg
     5881 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgggct
     5941 ggcgtaatag cgaagagggg atccccagga agctcctctg tgtcctcata aaccctaacc
     6001 tcctctactt gagaggacat tccaatcata ggcggccgcg aaggatctgc gatcgctccg
     6061 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     6121 tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     6181 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     6241 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg
     6301 ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga
     6361 gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa
     6421 gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac
     6481 tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt
     6541 tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgctt attcgtgccg
     6601 tgttatatga aaaccttcaa catctctcag caggatctgg agctggtgga ggtcgccact
     6661 gagaagatca ccatgctcta tgaggacaac aagcaccatg tcggggcggc catcaggacc
     6721 aagactgggg agatcatctc tgctgtccac attgaagcct acattggcag ggtcactgtc
     6781 tgtgctgaag ccattgccat tgggtctgct gtgagcaacg ggcagaagga ctttgacacc
     6841 attgtggctg tcaggcaccc ctactctgat gaggtggaca gatccatcag ggtggtcagc
     6901 ccctgtggca tgtgtagaga gctgatctct gactatgctc ctgactgctt tgtgctcatt
     6961 gagatgaatg gcaagctggt caaaaccacc attgaggaac tcatccccct caagtacacc
     7021 aggaactaag ggcacagcaa tcaaaagtag tcgacaatca acctctggat tacaaaattt
     7081 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     7141 ctttaatgcc tttgtatcat gcgttaacta aacttgttta ttgcagctta taatggttac
     7201 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7261 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggaattgact caaatgatgt
     7321 caattagtct atcagaagct catctggtct cccttccggg ggacaagaca tccctgttta
     7381 atatttaaac agcagtgttc ccaaactggg ttcttatatc ccttgctctg gtcaaccagg
     7441 ttgcagggtt tcctgtcctc acaggaacga agtccctaaa gaaacagtgg cagccaggtt
     7501 tagccccgga attgactgga ttcctttttt agggcccatt ggtatggctt tttccccgta
     7561 tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga aagcgatccc
     7621 gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg atgcgggggg
     7681 agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg ggggagggac
     7741 gtaattacat ccctgggggc tttggggggg ggctgtccct gatatctata acaagaaaat
     7801 atatatataa taagttatca cgtaagtaga acatgaaata acaatataat tatcgtatga
     7861 gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca cgcggtcgtt
     7921 atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg agctccaagc
     7981 ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa agagagagca
     8041 atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt taatctagct
     8101 gcatcaggat catatcgtcg ggtctttttt ccggctcagt catcgcccaa gctggcgcta
     8161 tctgggcatc ggggaggaag aagcccgtgc cttttcccgc gaggttgaag cggcatggaa
     8221 agagtttgcc gaggatgact gctgctgcat tgacgttgag cgaaaacgca cgtttaccat
     8281 gatgattcgg gaaggtgtgg ccatgcacgc ctttaacggt gaactgttcg ttcaggccac
     8341 ctgggatacc agttcgtcgc ggcttttccg gacacagttc cggatggtca gcccgaagcg
     8401 catcagcaac ccgaacaata ccggcgacag ccggaactgc cgtgccggtg tgcagattaa
     8461 tgacagcggt gcggcgctgg gatattacgt cagcgaggac gggtatcctg gctggatgcc
     8521 gcagaaatgg acatggatac cccgtgagtt acccggcggg cgcgcttggc gtaatcatgg
     8581 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8641 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     8701 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     8761 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     8821 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8881 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8941 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     9001 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9061 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9121 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     9181 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9241 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9301 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9361 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9421 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9481 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9541 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9601 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9661 atcttcacct agatcctttt aaattaaa
//
