LOCUS       sequence-249183-       10877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10877)
  AUTHORS   Dans M, Gagnoux-Palacios L, Blaikie P, Klein S, Mariotti A, 
            Giancotti FG
  TITLE     Tyrosine phosphorylation of the beta 4 integrin cytoplasmic domain 
            mediates Shc signaling to extracellular signal-regulated kinase and 
            antagonizes formation of hemidesmosomes.
  JOURNAL   J Biol Chem. 2001 Jan 12. 276(2):1494-502.
  PUBMED    11044453
REFERENCE   2  (bases 1 to 10877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2001 Jan 12. 276(2):1494-502."
COMMENT     Sequence Label: pcDNA3.1/Myc-His beta4
FEATURES             Location/Qualifiers
     source          1..10877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6362..6391
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6407..6424
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6468..6485)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6474..6698
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6744..7172
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6831..6850)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7041..7062
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7181..7201)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7186..7515
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7366..7501
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7428..7447
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7582..8376
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7636..7655)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8246..8265
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8550..8671
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8587..8606)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8641..8660
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8720..8736)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8720..8736)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8733..8755)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8744..8760
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8768..8774,8775..8792,8793..8798))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8813..8834
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8951..8968)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9122..9710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9202..9221)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9881..10672,10673..10741))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10504..10523
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10742..10846)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcg
      961 gcgcccgcgc gctgcagccc catctcctag cggcagccca ggcgcggagg gagcgagtcc
     1021 gccccgaggt aggtccagga cgggcgcaca gcagcagccg aggctggccg ggagagggag
     1081 gaagaggatg gcagggccac gccccagccc atgggccagg ctgctcctgg cagccttgat
     1141 cagcgtcagc ctctctggga ccttggcaaa ccgctgcaag aaggccccag tgaagagctg
     1201 cacggagtgt gtccgtgtgg ataaggactg cgcctactgc acagacgaga tgttcaggga
     1261 ccggcgctgc aacacccagg cggagctgct ggccgcgggc tgccagcggg agagcatcgt
     1321 ggtcatggag agcagcttcc aaatcacaga ggagacccag attgacacca ccctgcggcg
     1381 cagccagatg tccccccaag gcctgcgggt ccgtctgcgg cccggtgagg agcggcattt
     1441 tgagctggag gtgtttgagc cactggagag ccccgtggac ctgtacatcc tcatggactt
     1501 ctccaactcc atgtccgatg atctggacaa cctcaagaag atggggcaga acctggctcg
     1561 ggtcctgagc cagctcacca gcgactacac tattggattt ggcaagtttg tggacaaagt
     1621 cagcgtcccg cagacggaca tgaggcctga gaagctgaag gagccctggc ccaacagtga
     1681 cccccccttc tccttcaaga acgtcatcag cctgacagaa gatgtggatg agttccggaa
     1741 taaactgcag ggagagcgga tctcaggcaa cctggatgct cctgagggcg gcttcgatgc
     1801 catcctgcag acagctgtgt gcacgaggga cattggctgg cgcccggaca gcacccacct
     1861 gctggtcttc tccaccgagt cagccttcca ctatgaggct gatggcgcca acgtgctggc
     1921 tggcatcatg agccgcaacg atgaacggtg ccacctggac accacgggca cctacaccca
     1981 gtacaggaca caggactacc cgtcggtgcc caccctggtg cgcctgctcg ccaagcacaa
     2041 catcatcccc atctttgctg tcaccaacta ctcctatagc tactacgaga agcttcacac
     2101 ctatttccct gtctcctcac tgggggtgct gcaggaggac tcgtccaaca tcgtggagct
     2161 gctggaggag gccttcaatc ggatccgctc caacctggac atccgggccc tagacagccc
     2221 ccgaggcctt cggacagagg tcacctccaa gatgttccag aagacgagga ctgggtcctt
     2281 tcacatccgg cggggggaag tgggtatata ccaggtgcag ctgcgggccc ttgagcacgt
     2341 ggatgggacg cacgtgtgcc agctgccgga ggaccagaag ggcaacatcc atctgaaacc
     2401 ttccttctcc gacggcctca agatggacgc gggcatcatc tgtgatgtgt gcacctgcga
     2461 gctgcaaaaa gaggtgcggt cagctcgctg cagcttcaac ggagacttcg tgtgcggaca
     2521 gtgtgtgtgc agcgagggct ggagtggcca gacctgcaac tgctccaccg gctctctgag
     2581 tgacattcag ccctgcctgc gggagggcga ggacaagccg tgctccggcc gtggggagtg
     2641 ccagtgcggg cactgtgtgt gctacggcga aggccgctac gagggtcagt tctgcgagta
     2701 tgacaacttc cagtgtcccc gcacttccgg gttcctctgc aatgaccgag gacgctgctc
     2761 catgggccag tgtgtgtgtg agcctggttg gacaggccca agctgtgact gtcccctcag
     2821 caatgccacc tgcatcgaca gcaatggggg catctgtaat ggacgtggcc actgtgagtg
     2881 tggccgctgc cactgccacc agcagtcgct ctacacggac accatctgcg agatcaacta
     2941 ctcggcgatc cacccgggcc tctgcgagga cctacgctcc tgcgtgcagt gccaggcgtg
     3001 gggcaccggc gagaagaagg ggcgcacgtg tgaggaatgc aacttcaagg tcaagatggt
     3061 ggacgagctt aagagagccg aggaggtggt ggtgcgctgc tccttccggg acgaggatga
     3121 cgactgcacc tacagctaca ccatggaagg tgacggcgcc cctgggccca acagcactgt
     3181 cctggtgcac aagaagaagg actgccctcc gggctccttc tggtggctca tccccctgct
     3241 cctcctcctc ctgccgctcc tggccctgct actgctgcta tgctggaagt actgtgcctg
     3301 ctgcaaggcc tgcctggcac ttctcccgtg ctgcaaccga ggtcacatgg tgggctttaa
     3361 ggaagaccac tacatgctgc gggagaacct gatggcctct gaccacttgg acacgcccat
     3421 gctgcgcagc gggaacctca agggccgtga cgtggtccgc tggaaggtca ccaacaacat
     3481 gcagcggcct ggctttgcca ctcatgccgc cagcatcaac cccacagagc tggtgcccta
     3541 cgggctgtcc ttgcgcctgg cccgcctttg caccgagaac ctgctgaagc ctgacactcg
     3601 ggagtgcgcc cagctgcgcc aggaggtgga ggagaacctg aacgaggtct acaggcagat
     3661 ctccggtgta cacaagctcc agcagaccaa gttccggcag cagcccaatg ccgggaaaaa
     3721 gcaagaccac accattgtgg acacagtgct gatggcgccc cgctcggcca agccggccct
     3781 gctgaagctt acagagaagc aggtggaaca gagggccttc cacgacctca aggtggcccc
     3841 cggctactac accctcactg cagaccagga cgcccggggc atggtggagt tccaggaggg
     3901 cgtggagctg gtggacgtac gggtgcccct ctttatccgg cctgaggatg acgacgagaa
     3961 gcagctgctg gtggaggcca tcgacgtgcc cgcaggcact gccaccctcg gccgccgcct
     4021 ggtaaacatc accatcatca aggagcaagc cagagacgtg gtgtcctttg agcagcctga
     4081 gttctcggtc agccgcgggg accaggtggc ccgcatccct gtcatccggc gtgtcctgga
     4141 cggcgggaag tcccaggtct cctaccgcac acaggatggc accgcgcagg gcaaccggga
     4201 ctacatcccc gtggagggtg agctgctgtt ccagcctggg gaggcctgga aagagctgca
     4261 ggtgaagctc ctggagctgc aagaagttga ctccctcctg cggggccgcc aggtccgccg
     4321 tttccacgtc cagctcagca accctaagtt tggggcccac ctgggccagc cccactccac
     4381 caccatcatc atcagggacc cagatgaact ggaccggagc ttcacgagtc agatgttgtc
     4441 atcacagcca ccccctcacg gcgacctggg cgccccgcag aaccccaatg ctaaggccgc
     4501 tgggtccagg aagatccatt tcaactggct gcccccttct ggcaagccaa tggggtacag
     4561 ggtaaagtac tggattcagg gtgactccga atccgaagcc cacctgctcg acagcaaggt
     4621 gccctcagtg gagctcacca acctgtaccc gtattgcgac tatgagatga aggtgtgcgc
     4681 ctacggggct cagggcgagg gaccctacag ctccctggtg tcctgccgca cccaccagga
     4741 agtgcccagc gagccagggc gtctggcctt caatgtcgtc tcctccacgg tgacccagct
     4801 gagctgggct gagccggctg agaccaacgg tgagatcaca gcctacgagg tctgctatgg
     4861 cctggtcaac gatgacaacc gacctattgg gcccatgaag aaagtgctgg ttgacaaccc
     4921 taagaaccgg atgctgctta ttgagaacct tcgggagtcc cagccctacc gctacacggt
     4981 gaaggcgcgc aacggggccg gctgggggcc tgagcgggag gccatcatca acctggccac
     5041 ccagcccaag aggcccatgt ccatccccat catccctgac atccctatcg tggacgccca
     5101 gagcggggag gactacgaca gcttccttat gtacagcgat gacgttctac gctctccatc
     5161 gggcagccag aggcccagcg tctccgatga cactgagcac ctggtgaatg gccggatgga
     5221 ctttgccttc ccgggcagca ccaactccct gcacaggatg accacgacca gtgctgctgc
     5281 ctatggcacc cacctgagcc cacacgtgcc ccaccgcgtg ctaagcacat cctccaccct
     5341 cacacgggac tacaactcac tgacccgctc agaacactca cactcgacca cactgccgag
     5401 ggactactcc accctcacct ccgtctcctc ccacgactct cgcctgactg ctggtgtgcc
     5461 cgacacgccc acccgcctgg tgttctctgc cctggggccc acatctctca gagtgagctg
     5521 gcaggagccg cggtgcgagc ggccgctgca gggctacagt gtggagtacc agctgctgaa
     5581 cggcggtgag ctgcatcggc tcaacatccc caaccctgcc cagacctcgg tggtggtgga
     5641 agacctcctg cccaaccact cctacgtgtt ccgcgtgcgg gcccagagcc aggaaggctg
     5701 gggccgagag cgtgagggtg tcatcaccat tgaatcccag gtgcacccgc agagcccact
     5761 gtgtcccctg ccaggctccg ccttcacttt gagcactccc agtgccccag gcccgctggt
     5821 gttcactgcc ctgagcccag actcgctgca gctgagctgg gagcggccac ggaggcccaa
     5881 tggggatatc gtcggctacc tggtgacctg tgagatggcc caaggaggag ggccagccac
     5941 cgcattccgg gtggatggag acagccccga gagccggctg accgtgccgg gcctcagcga
     6001 gaacgtgccc tacaagttca aggtgcaggc caggaccact gagggcttcg ggccagagcg
     6061 cgagggcatc atcaccatag agtcccagga tggaggaccc ttcccgcagc tgggcagccg
     6121 tgccgggctc ttccagcacc cgctgcaaag cgagtacagc agcatcacca ccacccacac
     6181 cagcgccacc gagcccttcc tagtggatgg gccgaccctg ggggcccagc acctggaggc
     6241 aggcggctcc ctcacccggc atgtgaccca ggagtttgtg agccggacac tgaccaccag
     6301 cggaaccctt agcacccaca tggaccaaca gttcttccaa acttcggtac caagctttct
     6361 agaacaaaaa ctcatctcag aagaggatct gaatagcgcc gtcgaccatc atcatcatca
     6421 tcattgagtt taaacggtct ccagcttaag tttaaaccgc tgatcagcct cgactgtgcc
     6481 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     6541 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     6601 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     6661 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     6721 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6781 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     6841 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     6901 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     6961 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7021 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7081 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     7141 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     7201 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7261 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7321 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     7381 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7441 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7501 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     7561 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     7621 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     7681 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     7741 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     7801 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     7861 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     7921 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     7981 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     8041 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     8101 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     8161 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     8221 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     8281 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     8341 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     8401 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     8461 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     8521 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     8581 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8641 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     8701 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8761 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8821 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8881 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8941 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     9001 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     9061 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9121 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9181 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9241 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9301 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9361 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9421 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9481 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9541 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     9601 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9661 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9721 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9781 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9841 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9901 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9961 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    10021 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    10081 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    10141 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    10201 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10261 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10321 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10381 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10441 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10501 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10561 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10621 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10681 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10741 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10801 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10861 aagtgccacc tgacgtc
//
