LOCUS       sequence-249190-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express E. coli GreB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Tetone LE, Friedman LJ, Osborne ML, Ravi H, Kyzer S, Stumper SK, 
            Mooney RA, Landick R, Gelles J
  TITLE     Dynamics of GreB-RNA polymerase interaction allow a proofreading 
            accessory protein to patrol for transcription complexes needing 
            rescue.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Feb 14;114(7):E1081-E1090. doi: 
            10.1073/pnas.1616525114. Epub 2017 Jan 30.
  PUBMED    28137878
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1616525114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-02-14- 14"; volume: "114"; issue: "7"; pages: 
            "E1081-E1090"
COMMENT     Sequence Label: pET15b-GB
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..32
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(562..580)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      576..623
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(798..803,804..820,821..826))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(853..871)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atcacagcag cggcctggtg ccgcgcggca gccatatgaa aacgcccctg gttacccggg
       61 aagggtatga aaaactcaaa caagagctta attatctctg gcgtgaagaa cgcccggagg
      121 tcacaaaaaa ggtgacctgg gccgcaagtc tgggcgaccg cagcgaaaat gctgactatc
      181 agtataataa aaagcgtctg cgtgaaatcg accgtcgcgt gcgctatctc actaaatgcc
      241 tggaaaatct caaaatcgtc gattactccc ctcagcagga aggcaaagtc ttttttggcg
      301 cgtgggtgga gattgaaaac gacgatggcg tgactcaccg tttccgtatt gtcggctacg
      361 atgaaatttt tggccgtaaa gattacatct ctatcgattc cccgatggcc cgcgcattgc
      421 tgaaaaaaga agtcggcgat ctggcggtgg tgaatacccc tgccggggaa gcgagctggt
      481 atgttaatgc tatcgagtac gtgaaaccgt aggatccggc tgctaacaaa gcccgaaagg
      541 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
      601 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat cccgcaagag
      661 gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag
      721 gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa
      781 ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt
      841 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataa
//
