LOCUS       sequence-249193-       10868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To maximize gene expression in B cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10868)
  AUTHORS   Martincic K, Alkan SA, Cheatle A, Borghesi L, Milcarek C
  TITLE     Transcription elongation factor ELL2 directs immunoglobulin 
            secretion in plasma cells by stimulating altered RNA processing.
  JOURNAL   Nat Immunol. 2009 Oct;10(10):1102-9. doi: 10.1038/ni.1786. Epub 2009
            Sep 13.
  PUBMED    19749764
REFERENCE   2  (bases 1 to 10868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.1786"; 
            journalName: "Nat Immunol"; date: "2009-10"; volume: "10"; issue: 
            "10"; pages: "1102-9"
COMMENT     Sequence Label: IgH intron enhancer
FEATURES             Location/Qualifiers
     source          1..10868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    3356..3490
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3380..3399)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3434..3453
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5159..5179)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          5309..5374
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(7847..8176)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7861..7996
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8161..8181
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(8219..8241)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8327..8467
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8382..8401
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8482..8499)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8653..9241)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8733..8752)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9412..10203,10204..10272))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10035..10054
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10273..10377)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10445..10463
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agtccacatg ctctgtgtga actccctctg gccctgctta ttgttgaatg ggccaaaggt
       61 ctgagaccag gctgctgctg ggtaggcctg gactttgggt ctcccaccca gacctgggaa
      121 tgtatggttg tggcttctgc cacccatcca cctggctgct catggaccag ccagcctcgg
      181 tggctttgaa ggaacaattc cacacaaaga ctctggacct ctccgaaacc aggcaccgca
      241 aatggtaagc cagaggcagc cacagctgtg gctgctgctc ttaaagcttg taaactgttt
      301 ctgcttaaga gggactgagt cttcagtcat tgctttaggg ggagaaagag acatttgtgt
      361 gtcttttgag taccgttgtc tgggtcactc acatttaact ttccttgaaa aactagtaaa
      421 agaaaaatgt tgcctgttaa ccaataatca tagagctcat ggtattttga ggaaatctta
      481 gaaaacgtgt atacaattgt ctggaattat ttcagttaag tgtattagtt gaggtactga
      541 tgctgtctct acttcagtta tacatgtggg tttgaatttt gaatctattc tggctcttct
      601 taagcagaaa atttagataa aatggatacc tcagtggttt ttaatggtgg gtttaatata
      661 gaaggaattt aaattggaag ctaatttaga atcagtaagg agggacccag gctaagaagg
      721 caatcctggg attctggaag aaaagatgtt tttagttttt atagaaaaca ctactacatt
      781 cttgatctac aactcaatgt ggtttaatga atttgaagtt gccagtaaat gtacttcctg
      841 gttgttaaag aatggtatca aaggacagtg cttagatcca aggtgagtgt gagaggacag
      901 gggctggggt atggatacgc agaaggaagg ccacagctgt acagaattga gaaagaatag
      961 agacctgcag ttgaggccag caggtcggct ggactaactc tccagccaca gtaatgaccc
     1021 agacagagaa ggccagactc ataaagcttg ctgagcaaaa ttaagggaac aaggttgaga
     1081 gccctagtaa gcgaggctct aaaaagcatg gctgagctga gatgggtggg cttctctgag
     1141 cgcttctaaa atgcgctaaa ctgaggtgat tactctgagg taagcaaagc tgggcttgag
     1201 ccaaaatgaa gtagactgta atgaactgga atgagctggg ccgctaagct aaactaggct
     1261 ggcttaagcg agatgagcca aactggaatg aacttcatta atctaggttg aatagagatg
     1321 tgaggtacca gtcctagcag ctatggggca actgggtata attggaatat gggggaccag
     1381 atctagcagc tatggaggag cagggatagg tgggagtatt agggaccagt cctatcagct
     1441 gtgggggagc tgaagatggt aggaatgtgg aggaccagac ctaagagtta ggagagagct
     1501 ggggcaggta ggagtatgag ggaccagtac taacagctat ggaggagttg gggattttgg
     1561 aaatgttgag agactagtcc tagcagctat ggggcaactg agtatagttt gaatatgggg
     1621 gactatatct agcagctatg gggaagcaag gataggtggg agtattaggg accaatccca
     1681 gcagctgtgg gggagttggg gatggtagca atgtggggaa ccagtcctag aagctatggg
     1741 ggatctggaa taggtagtag agtcagggac aagacatagc agctatgatg gagctggaga
     1801 aggtgggaat atgagggaga agtcctagca gctatggagg aactatggat catagaaatt
     1861 tgggggacca gttctagaag ttgtgggaga gctgaggcag gtggaagtgt gagggaccag
     1921 acctgacagc tatggagggc ttgggatgtt gaaaatgtga gggaccagtt ctagcagcta
     1981 tggggaatct tggtcaggtg agggtgattt taagggacca gacctagtag gtataggtct
     2041 gctgggcagg ttagagtgtc aggaaccaac tatgaaagac ctgagttagg taggagtgta
     2101 agggaccaga catagcagct aaggggaaat gtgataaatg ggagtgtaag ggaccagatt
     2161 cagtaaactt tggagtatag gtgagcatcc agaaaatctg tggtgcaact gtaacaggtg
     2221 gggtgtgagg aaccagaccc agtaagaatg ggatacctgg ggagagcggc atgtgaggga
     2281 ccaccagtgg ttaaggtagc aaacctgctc atggaaagct tccagagatg agagcagaga
     2341 acaggacagt gtaggatgtg caggatctag aagctcatct cagaggatga agaacagtca
     2401 aaagctctgc tcagaagttc ttcagggcga tgtgaacatg ctgggtactc aggaagtgac
     2461 aaggatcctc ccttacacac ctcataaaac aagagagagt gggggctcag gcagacacaa
     2521 cagctgtgcc aaaagtgaat aaggatgaaa agaggcaaca ggaagagaag aaaaagggat
     2581 gggaaagcac tccgggctct actgaattcc tttgcctaat ttaaatgagg acttaacctg
     2641 tggaaatatt ttgatgtggg aagctgttac tgttaaaact gaggttattg gggtaactgc
     2701 tatgttaaac ttgcattcag ggacacaaaa aactcatgaa aatggtgctg gaaaacccat
     2761 tcaagggtca aattttcatt tttttgctgt tggtggggaa cctttggagc tgcagggtgt
     2821 gttagcaaac tacaggacca aatatcctgc tcaaactgta accccaaaaa atgctacagt
     2881 tgacagtcag cagatgaaca ctgaccacaa ggctgttttg gataaggata atgcttatcc
     2941 agtggagtgc tgggttcctg atccaagtaa aaatgaaaac actagatatt ttggaaccta
     3001 cacaggtggg gaaaatgtgc ctcctgtttt gcacattact aacacagcaa ccacagtgct
     3061 tcttgatgag cagggtgttg ggcccttgtg caaagctgac agcttgtatg tttctgctgt
     3121 tgacatttgt gggctgttta ccaacacttc tggaacacag cagtggaagg gacttcccag
     3181 atattttaaa attaccctta gaaagcggtc tgtgaaaaac ccctacccaa tttccttttt
     3241 gttaagtgac ctaattaaca ggaggacaca gagggtggat gggcagccta tgattggaat
     3301 gtcctctcaa gtagaggagg ttagggttta tgaggacaca gaggagcttc ctggggatcc
     3361 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     3421 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     3481 taaacaagtt ggtcgactct agaggatccc cggggcctgc tttccttcct ttccgtctgg
     3541 gacatcagtc tcagcttccc acctgagtcc aataatgcct tgggtggacc tcagagtgac
     3601 ctggattatt caagtgccct ctatgcttgt ctcatctttc attccttcag tacaggagaa
     3661 ggtggagggt cagcagggcc tgtgacccag gtcaacagtg gctatacagc tggactgacc
     3721 ctttgctgtc cttcagtcat cagcagagcc tgtggctcgt cttgcctgaa cttgggaaca
     3781 tcccagtgtg aagtgattca gacaattatg gctatggttt gaattattca tgtggggttg
     3841 aagcatgacc ccgaggtacc agagatggat ctggcttcag aggtcttgtg ttgagcatat
     3901 aatgcagtgg ccacagaaga ggaaaggtgt gtctgattgt gatgaaagaa acgtgggcat
     3961 tgaatttttg gctgagtgta tggtgataca ttgctggatg taggtgctgt gcagggcaca
     4021 gaggagttgt cccagtgtgt taaatgtgtg tgtgctcacg agcatgagga tacatgtgct
     4081 atatggactg gatggtgttc agcagggtca ggatttagca tgagtgtggc tatctaatag
     4141 tccctccctt ggggcatcaa gagccatgtg cctcagcccc aggtggcaca ggcaggagtg
     4201 agccatttgt atgggtgtgc agacctcgtc tgtgaaaagc tgcctgagtt gagagtatag
     4261 atgacaaagg tctatggaat gcccctggcc atgtgtcact ccacaataga ctcagaaaaa
     4321 cccctaagta gagaaatggg gcagggggac aggaacagcc taagggaggt gggcttgtgt
     4381 gcttatccat ttgaggcttc ctaggattat ctctatgtct ggcagctgct ggttcatggg
     4441 aaggcaagga aaaggcactg cctaaagcca agccccaaca gtccaaggca gaatgactca
     4501 tgacctctga gtcatgcagt gcagaaacct cattctcttc ctatagacaa ggctgttctc
     4561 caaccctggc cagacctatc agcccgtgag ctatgctcta taccttggaa agaaccagga
     4621 cagttttaca tggtaccagg ctttgctgga tgctttattt atacaaggga tgcatggata
     4681 tgggtgccag tgtctcttag gacctgagag ctttgtgggt gctgagctcg aattaacaac
     4741 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     4801 aagtaaaacc tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca
     4861 tcaaatattc cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag
     4921 catagttatt aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat
     4981 gattataact gttatgccta cttataaagg ttacagaata tttttccata attttcttgt
     5041 atagcagtgc agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac
     5101 taaacacagc atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta
     5161 cctttctctt cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac
     5221 tagatggcat ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct
     5281 cccattcatc agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt
     5341 taacacatta tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta
     5401 gtttgtccaa ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cggggcccac
     5461 tcataaatcc agttgccgcc acggtagcca atcaccgtat cgtataaatc atcgtcggta
     5521 cgttcggcat cgctcatcac aatacgtgcc tggacgtcga ggatttcgcg tgggtcaatg
     5581 ccgcgccaga tccacatcag acggttaatc atgcgatacc agtgagggat ggttttacca
     5641 tcaagggccg actgcacagg cggttgtgcg ccgtgattaa agcggcggac tagcgtcgag
     5701 gtttcaggat gtttaaagcg gggtttgaac agggtttcgc tcaggtttgc ctgtgtcatg
     5761 gatgcagcct ccagaatact tactggaaac tattgtaacc cgcctgaagt taaaaagaac
     5821 aacgcccggc agtgccaggc gttgaaaaga ttagcgaccg gagattggcg ggacgaatac
     5881 gacgcccata tcccacggct gttcaatcca ggtatcttgc gggatatcaa caacatagtc
     5941 atcaaccagc ggacgaccag ccggttttgc gaagatggtg acaaagtgcg cttttggata
     6001 catttcacga atcgcaaccg cagtaccacc ggtatccacc aggtcatcaa taacgatgaa
     6061 gccttcgcca tcgccttctg cgcgtttcag cactttaagc tcgcgctggt tgtcgtgatc
     6121 gtagctggaa atacaaacgg tatcgacatg acgaataccc agttcacgcg ccagtaacgc
     6181 acccggtacc agaccgccac ggcttacggc aataatgcct ttccattgtt cagaaggcat
     6241 cagtcggctt gcgagtttac gtgcatggat ctgcaacatg tcccaggtga cgatgtattt
     6301 ttcgctcatg tgaagtgctg atgaatcccc taatgatttt tatcaaaatc attaagttaa
     6361 ggtagataca catcttgtca tatgatcaaa tggtttcgcc aaaaatcaat aatcagacaa
     6421 caaaatgtgc gaactcgata ttttacacga ctctctttac caattctgcc ccgaattaca
     6481 cttaaaacga ctcaacagct taacgttggc ttgccacgcc ttacttgact gtaaaactct
     6541 cactcttacc gaacttggcc gtaacctgcc aaccaaagcg agaacaaaac ataacatcaa
     6601 acgaatcgac cgattgttag gtaatcgtca cctccacaaa gagcgactcg ctgtataccg
     6661 ttggcatgct agctttatct gttcgggcaa tacgatgccc attgtacttg ttgactggtc
     6721 tgatatccgt gagcaaaaac ggcttatggt attgcgagct tcagtcgcac tacacggtcg
     6781 ttctgttact ctttatgaga aagcgttccc gctttcagag caatgttcaa agaaagctca
     6841 tgaccaattt ctagccgacc ttgcgagcat tctaccgagt aacaccacac cgctcattgt
     6901 cagtgatgct ggctttaaag tgccatggta taaatccgtt gagaagctgg gttggtactg
     6961 gttaagtcga gtaagaggaa aagtacaata tgcagaccta ggagcggaaa actggaaacc
     7021 tatcagcaac ttacatgata tgtcatctag tcactcaaag actttaggct ataagaggct
     7081 gactaaaagc aacccaatct catgccaaat tctattgtat aaatctcgct ctaaaggccg
     7141 aaaaaatcag cgctcgacac ggactcattg tcaccacccg tcacctaaaa tctactcagc
     7201 gtcggcaaag gagccatgga ttctagcaac taacttacct gttgaaattc gaacacccaa
     7261 acaacttgtt aatatctatt cgaagcgaat gcagattgaa gaaaccttcc gagacttgaa
     7321 aagtcctgcc tacggactag gcctacgcca tagccgaacg agcagctcag agcgttttga
     7381 tatcatgctg ctaatcgccc tgatgcttca actaacatgt tggcttgcgg gcgttcatgc
     7441 tcagaaacaa ggttgggaca agcacttcca ggctaacaca gtcagaaatc gaaacgtact
     7501 ctcaacagtt cgcttaggca tggaagtttt gcggcattct ggctacacaa taacaaggga
     7561 agactcactc gtggctgcaa ccctgcttac tcaaaatcta ttcacacatg gttacgtttt
     7621 ggggaaatta tgaggggatc tctcagtgtg aagtgtccca gcctgtttat ctacggctta
     7681 aaaagtgttc gaggggaaaa taggttgcgc gagattatag agatctggcg cactaaaaac
     7741 cagtatttca catgagtccg cgtcttttta cgcactgcct ctccctgacg cgggataaag
     7801 tggtattctc aaacatatct cgcaagcctg tcttgtgtcc aagctttttg caaaagccta
     7861 ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg
     7921 cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg
     7981 gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat
     8041 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg
     8101 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     8161 accctaactg acacacattc cacagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     8221 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     8281 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     8341 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     8401 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     8461 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8521 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8581 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8641 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8701 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8761 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8821 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8881 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8941 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     9001 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     9061 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     9121 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9181 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9241 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9301 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9361 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9421 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9481 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9541 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9601 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9661 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9721 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9781 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9841 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9901 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9961 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10021 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10081 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10141 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10201 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10261 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10321 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10381 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
    10441 taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttcattttca aaattaggtt
    10501 atgtaagaaa ttgaaggact ttagtgtctt taatttctaa tatatttaga aaacttctta
    10561 aaattactct attattcttc cctctgatta ttggtctcca ttcaattctt ttccaatacc
    10621 cgaagcattt acagtgactt tgttcatgat cttttttagt tgtttgtttt gccttactat
    10681 taagactttg acattctggt caaaacggct tcacaaatct ttttcaagac cactttctga
    10741 gtattcattt taggagaaat actttttttt taaatgaatg caattatcta gacttatttc
    10801 ggttgaacat gctggttggt ggttgagagg acactcagtc agtcagtggc gtgaagggct
    10861 tctaagcc
//
