LOCUS       sequence-24923-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Oka T, Mazack V, Sudol M
  TITLE     Mst2 and Lats kinases regulate apoptotic function of Yes 
            kinase-associated protein (YAP).
  JOURNAL   J Biol Chem. 2008 Oct 10;283(41):27534-27546. doi: 
            10.1074/jbc.M804380200. Epub 2008 Jul 17.
  PUBMED    18640976
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M804380200"; journalName: "J Biol Chem"; date: 
            "2008-10-10- 10"; volume: "283"; issue: "41"; pages: "27534-27546"
COMMENT     Sequence Label: pcDNA4/HisMaxB-YAP1-S61A
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..180
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             193..210
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             214..246
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(250..258,259..273)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 tacgtttact taagcttagc gcagaggctt ggggcagccg agcggcagcc aggccccggc
       61 ccgggnctcg gttccagaag ggagaggagc ccgccaaggc gcgcaagaga gcgggctgcc
      121 tcgcagtccg agccggagag ggagcgcgag ccgcgccggc cccggacggc ctccgaaacc
      181 atggggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa
      241 atgggtcggg atctgtacga cgatgacgat aaggtaccta aggatcccgg gcagcagccg
      301 ccgcctcaac cggcccccca gggccaaggg cagccgcctt cgcagccccc gcaggggcag
      361 ggcccgccgt ccggacccgg gcaaccggca cccgcggcga cccaggcggc gccgcaggca
      421 ccccccgccg ggcatcagat cgtgcacgtc cgcggggacg cggagaccga cctggaggcg
      481 ctcttcaacg ccgtcatgaa ccccaagacg gccaacgtgc cccagaccgt gcccatgagg
      541 ctccggaagc tgcccgactc cttcttcaag ccgccggagc ccaaatccca ctcccgacag
      601 gccagtactg atgcaggcac tgcaggagcc ctgactccac agcatgttcg agctcattcc
      661 tctccagctt ctctgcagtt gggagctgtt tcttctgnga nactnactcn cactggatta
      721 atattttttt ggccanaant tanaccaaan tttaatattt ttnaaanttt ttttttgaat
      781 acttgntant ttnttttnca caggttggaa atgcaanttn ntttgttcaa antttntaa
//
