LOCUS       sequence-24925-e         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Oka T, Mazack V, Sudol M
  TITLE     Mst2 and Lats kinases regulate apoptotic function of Yes 
            kinase-associated protein (YAP).
  JOURNAL   J Biol Chem. 2008 Oct 10;283(41):27534-27546. doi: 
            10.1074/jbc.M804380200. Epub 2008 Jul 17.
  PUBMED    18640976
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M804380200"; journalName: "J Biol Chem"; date: 
            "2008-10-10- 10"; volume: "283"; issue: "41"; pages: "27534-27546"
COMMENT     Sequence Label: pcDNA4/HisMaxB-YAP1-S109A
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    22..184
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             197..214
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             218..250
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(254..262,263..277)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 cantgctcgt tnacttagct tagcgcagag gcttggggca gccgagcggc agccaggccc
       61 cggcccgggn ctcggttcca gaagggagag gagcccgcca aggcgcgcaa gagagcgggc
      121 tgcctcgcag tccgagccgg agagggagcg cgagccgcgc cggccccgga cggcctccga
      181 aaccatgggg ggttctcatc atcatcatca tcatggtatg gctagcatga ctggtggaca
      241 gcaaatgggt cgggatctgt acgacgatga cgataaggta cctaaggatc ccgggcagca
      301 gccgccgcct caaccggccc cccagggcca agggcagccg ccttcgcagc ccccgcaggg
      361 gcagggcccg ccgtccggac ccgggcaacc ggcacccgcg gcgacccagg cggcgccgca
      421 ggcacccccc gccgggcatc agatcgtgca cgtccgcggg gactcggaga ccgacctgga
      481 ggcgctcttc aacgccgtca tgaaccccaa gacggccaac gtgccccaga ccgtgcccat
      541 gaggctccgg aagctgcccg actccttctt caagccgccg gagcccaaat cccactcccg
      601 acaggccgct actgatgcag gcactgcagg agccctgact ccacagcatg ttcgagctca
      661 ttcctctcca gcttctctgc agttgggagc tgtttctcct gggacactga cccccactgg
      721 agtagtctct ggcccagcag ctacacccac agctcagcat cttcgacagt cttttttaan
      781 nncctgatna tttncncttg cnannagntt gga
//
