LOCUS       sequence-24927-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Oka T, Mazack V, Sudol M
  TITLE     Mst2 and Lats kinases regulate apoptotic function of Yes 
            kinase-associated protein (YAP).
  JOURNAL   J Biol Chem. 2008 Oct 10;283(41):27534-27546. doi: 
            10.1074/jbc.M804380200. Epub 2008 Jul 17.
  PUBMED    18640976
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M804380200"; journalName: "J Biol Chem"; date: 
            "2008-10-10- 10"; volume: "283"; issue: "41"; pages: "27534-27546"
COMMENT     Sequence Label: pcDNA4/HisMaxB-YAP1-S163A
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    7..169
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             182..199
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             203..235
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(239..247,248..262)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     gap             774..809
                     /estimated_length=36
     gap             841..855
                     /estimated_length=15
ORIGIN
        1 aagcttagcg cagaggcttg gggcagccga gcggcagcca ggccccggcc cgggcctcgg
       61 ttccagaagg gagaggagcc cgccaaggcg cgcaagagag cgggctgcct cgcagtccga
      121 gccggagagg gagcgcgagc cgcgccggcc ccggacggcc tccgaaacca tggggggttc
      181 tcatcatcat catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga
      241 tctgtacgac gatgacgata aggtacctaa ggatcccggg cagcagccgc cgcctcaacc
      301 ggccccccag ggccaagggc agccgccttc gcagcccccg caggggcagg gcccgccgtc
      361 cggacccggg caaccggcac ccgcggcgac ccaggcggcg ccgcaggcac cccccgccgg
      421 gcatcagatc gtgcacgtcc gcggggactc ggagaccgac ctggaggcgc tcttcaacgc
      481 cgtcatgaac cccaagacgg ccaacgtgcc ccagaccgtg cccatgaggc tccggaagct
      541 gcccgactcc ttcttcaagc cgccggagcc caaatcccac tcccgacagg ccagtactga
      601 tgcaggcact gcangagccc tgactccaca gcatgttcna gctcattcct ctccagcttc
      661 tcnnnnnntg gganctgttt ctcctgggan actgaccccc actgnagtag tctctggccc
      721 agcanctaca cccncagctc ancatcttcn nnnnggnnnc nnntgagata nctnnnnnnn
      781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aannnnnnnt nnnggnnnan nnnnntnntt
      841 nnnnnnnnnn nnnnnannnn aa
//
