LOCUS       sequence-24934-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Oka T, Mazack V, Sudol M
  TITLE     Mst2 and Lats kinases regulate apoptotic function of Yes 
            kinase-associated protein (YAP).
  JOURNAL   J Biol Chem. 2008 Oct 10;283(41):27534-27546. doi: 
            10.1074/jbc.M804380200. Epub 2008 Jul 17.
  PUBMED    18640976
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M804380200"; journalName: "J Biol Chem"; date: 
            "2008-10-10- 10"; volume: "283"; issue: "41"; pages: "27534-27546"
COMMENT     Sequence Label: pcDNA4/HisMaxB-YAP1-T110A
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    16..179
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             192..209
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             213..245
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(249..257,258..272)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 tncgttactt agcttagcgc agaggcttgg ggcagccgag cggcagccag gccccggccc
       61 gggttttcgg ttccagaagg gagaggagcc cgccaaggcg cgcaagagag cgggctgcct
      121 cgcagtccga gccggagagg gagcgcgagc cgcgccggcc ccggacggcc tccgaaacca
      181 tggggggttc tcatcatcat catcatcatg gtatggctag catgactggt ggacagcaaa
      241 tgggtcggga tctgtacgac gatgacgata aggtacctaa ggatcccggg cagcagccgc
      301 cgcctcaacc ggccccccag ggccaagggc agccgccttc gcagcccccg caggggcagg
      361 gcccgccgtc cggacccggg caaccggcac ccgcggcgac ccaggcggcg ccgcaggcac
      421 cccccgccgg gcatcagatc gtgcacgtcc gcggggactc ggagaccgac ctggaggcgc
      481 tcttcaacgc cgtcatgaac cccaagacgg ccaacgtgcc ccagaccgtg cccatgaggc
      541 tccggaagct gcccgactcc ttcttcaagc cgccggagcc caaatcccac tcccgacagg
      601 ccagtgctga tgcaggcact gcaggagccc tgactccaca gcatgttcga nctcattcct
      661 ctccancttc tctgcagttg ggagctgttt ctcctgggac actgaccccc actggagtag
      721 tctctggccn gcagctncac ccacagctca ncatcttcan agtctttttt naatnnctna
      781 ngatnacttn ncagnaggtg ggantgnaaa aattttttgg tcaaaatgtc ttantcntnn
      841 atttcnanac tgc
//
