LOCUS       sequence-249444-       13667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PPI (BiFC/TriFC) assay vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13667)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 13667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-503    C-Tag GFP[1] L32 Piezo1 mCherry
FEATURES             Location/Qualifiers
     source          1..13667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        153..532
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        533..736
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     687..707
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     733..757
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          872..1004
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     regulatory      1019..1028
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(8835..9409,9410..9412,9413..9421)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9002..9019)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9229..9248
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9422..10132
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9567..9585)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9835..9854
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     intron          10178..10310
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    10319..10907
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10372..10392)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10790..10801)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10934..10951)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10940..11051
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(11147..11167)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11147..11165)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11186..11202)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11186..11202)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11199..11221)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     complement(11304..11323)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    11369..11424
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(11423..11442)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(11803..12391)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11883..11902)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12562..13353,13354..13422))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13185..13204
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13423..13527)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13595..13613
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcctgcctcc aggcctcaat attggccatt agccatatta ttcattggtt atatagcata
       61 aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
      121 tattggctca tgtccaatat gaccgccatg ttggcattga ttattgacta gttattaata
      181 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      241 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      301 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      361 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
      421 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
      481 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      541 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      601 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      661 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt
      721 ctatataagc agagctcgtt tagtgaaccg tcagatcact agaagcttta ttgcggtagt
      781 ttatcacagt taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag
      841 ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta
      901 aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc
      961 acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccaggc
     1021 caccatggag ccgcacgtgc tgggcgccgg gctctactgg ctgttgctgc cctgcacgct
     1081 cctggcggcc tccctgttac gcttcaatgc tctctcgctg gtctatttgc tgtttctact
     1141 gctgctgccc tggcttccag gcccctcaag acacagcata ccaggtcaca caggtcgcct
     1201 gctccgtgca ctactctgcc tcagcctcct cttcctggtg gcccaccttg cctttcagat
     1261 atgcctacac accgtgcctc acctggacca gtttctggga caaaacggta gcctttgggt
     1321 gaaggtgtct caacacatag gggttacgag gctggacctg aaggacatct ttaacaccac
     1381 caggctggta gcacctgacc tgggagtgct gctggcgtcc tccctttgcc ttggcctctg
     1441 tggacgcctc acgaggaaag ccgggcagag tcggcgcacc caggagctgc aggatgatga
     1501 cgatgacgat gatgacgacg atgaagacat agatgctgcc ccagccgtgg ggctgaaggg
     1561 agcccctgcc ctggcaacca aacgcaggct gtggctggcc tcccgcttcc gggtcacggc
     1621 ccactggctg ctgatgacct ctggacggac gctggtcatt gtgttgctgg ccctggcagg
     1681 catagcccac ccttcggcct tctccagcat ctacctggtg gtgttcctgg ccatctgcac
     1741 ctggtggtcc tgtcactttc ctctcagccc cctgggcttt aacaccctct gtgtcatggt
     1801 gagctgtttt ggtgccggcc atctcatttg cctatactgc tatcagacac catttatcca
     1861 ggacatgtta ccccctggga acatctgggc caggctattt ggtctcaaga acttcgtaga
     1921 cctccctaac tactccagcc ccaacgccct ggtactcaac actaagcacg cctggcccat
     1981 ctatgtgagt cccggaatcc tgctgctgct atattacaca gccacctctc tcctgaagct
     2041 ccacaagagc tgtccctcag agctgaggaa ggagacaccc agggaggatg aggagcatga
     2101 gctggaactg gaccacctgg agccagagcc ccaggctagg gacgccaccc agggtgagat
     2161 gcccatgacc acggaacctg accttgacaa ctgcaccgtg catgtactaa ccagccagag
     2221 ccctgtccgc cagcgtccag ttcgccccag gctggctgag ctgaaagaga tgtcaccgct
     2281 acatggcctg ggccacctca tcatggacca gagctatgtg tgtgccctga ttgccatgat
     2341 ggtgtggagc atcatgtacc acagttggct gaccttcgtc ctgctgctct gggcctgcct
     2401 catctggacg gtgcgtagcc gtcaccagct ggctatgctc tgctcgccct gcatcctgct
     2461 gtatgggctg acgctctgct gcctgaggta tgtgtgggcc atggaacttc ctgagctgcc
     2521 caccaccctg ggccctgtca gcctgcacca gttgggactg gaacacacac gctacccttg
     2581 cctggacctc ggtgccatgc tcctctatct gctcacattc tggctccttc tgcgtcagtt
     2641 cgtgaaggag aagctgctga agaagcaaaa ggtgcctgcg gcgctgctgg aggtcacggt
     2701 ggccgacact gagcccacac agacccagac gctgctgcgg agcctggggg agctggtcac
     2761 cggcatctac gtcaaatact ggatctatgt gtgcgccggc atgttcattg tggtcagctt
     2821 cgccggccgc ctggtggtct acaaaatcgt ctacatgttc ctcttcctgc tgtgcctcac
     2881 cttgttccag gtctactaca ccctgtggag gaagctgcta cgtgtcttct ggtggctcgt
     2941 ggtggcctat acaatgctcg tgctcatcgc tgtgtacacc ttccagttcc aggacttccc
     3001 cacctattgg cgcaacctca cgggcttcac agacgagcag ttgggcgacc tgggcctgga
     3061 gcagttcagt gtgtcggagc tcttttccag tatcctcatc cctggcttct tcctgctggc
     3121 ctgcatcctg cagctgcact acttccacag accgttcatg cagctcactg acctggagca
     3181 cgtgccgcca ccaggcaccc gccaccctcg atgggctcac aggcaggatg cagtgagcga
     3241 ggcccctctg cttgagcatc aggaggaaga ggaagtcttc agggaagatg ggcagagcat
     3301 ggatgggccc caccaggcca cacaggtccc tgagggtacg gccagcaagt ggggcctggt
     3361 ggctgaccgg ctgctggacc tagcggccag cttctcggct gtcctcaccc gaatccaggt
     3421 gttcgttcgg cgcttgctag aacttcacgt cttcaagctg gtggccctct acactgtctg
     3481 ggtggccctg aaagaagtgt ctgtgatgaa cctgctgctg gtggtgctat gggccttcgc
     3541 cttgccctat ccgcgcttcc ggcccatggc ttcctgcctg tccaccgtgt ggacctgtat
     3601 catcattgta tgcaagatgc tctatcagct caagattgtc aacccgcatg agtactccag
     3661 caactgcact gagcccttcc ccaacaatac caacttgcag cctttggaga tcaaccagtc
     3721 tttgctgtac cgtggccctg ttgaccctgc caactggttt ggggtgcgga agggttaccc
     3781 caacttgggc tatatccaga accacctgca gatccttctg ttgctggtgt ttgaggccgt
     3841 ggtgtaccgg cgccaagagc actaccgccg gcagcaccag caggcccctc tgcccgccca
     3901 ggctgtgtgc gcagatggca cccgccagag gttggaccag gacctactta gctgcctcaa
     3961 gtatttcatc aacttcttct tctacaaatt cgggctggag atctgcttct tgatggccgt
     4021 gaatgtgatt gggcagcgta tgaacttcat ggtgatctta cacggttgct ggttggtggc
     4081 catccttaca cgccggcgcc gtgaggccat cgcccgcctc tggcctaact actgtctgtt
     4141 cctcacgctg ttcctgctgt accagtacct gctgtgtttg ggcatgcccc ctgcactgtg
     4201 cattgactat ccatggcgct ggagcaaggc catccccatg aattccgccc tcatcaagtg
     4261 gctgtaccta cctgacttct tcagagcccc caactccacc aaccttatca gtgacttcct
     4321 cctgctgctt tgcgcctccc agcagtggca ggtcttctca gcggagcgaa cggaggagtg
     4381 gcaacgcatg gcgggcatca acactgacca cctggagccc ctgcgtgggg agcccaaccc
     4441 tatacccaac ttcatccact gcaggtccta tctggatatg ctgaaggtgg ccgtcttccg
     4501 ctacctgttc tggctggtgc tcgttgtggt gtttgttgcg ggggccaccc gcataagcat
     4561 cttcgggctg gggtacctgc tagcctgctt ctacctgctg ctgtttggca ctaccctgct
     4621 gcagaaggac acgcgagccc agctcgtgct gtgggactgc ctcatcctct ataatgtcac
     4681 tgtcatcatc tctaagaata tgctgtcgct cctgtcctgt gtcttcgtgg agcaaatgca
     4741 gagcaacttc tgctgggtca tccagctctt cagcctcgtg tgcacagtca aaggctacta
     4801 tgatcccaaa gagatgatga ccagggaccg ggactgcctg ctgcctgtgg aggaggccgg
     4861 gatcatctgg gacagtatct gcttcttctt cctgctcttg caacggcgca tctttctcag
     4921 ccactacttc ctgcatgtca gcgctgacct gaaagccaca gccctgcagg catccagggg
     4981 ctttgccctc tacaatgcag ccaacctgaa gagcatcaac ttccatcgcc agattgagga
     5041 gaagtccctg gcccagctga aaagacagat gaagcgcatc cgtgccaaac aggagaagta
     5101 caggcagagc caggcaagtc gtggccaact ccagtccaaa gaccctcagg atcccagcca
     5161 ggagccaggg cctgacagcc cagggggctc ctccccgcca cggagacagt ggtggcgccc
     5221 ctggctggac cacgccacag tcatccactc tggcgactac ttcctgtttg agtcagatag
     5281 cgaggaggaa gaggaggccc tacctgagga ccccaggcct gcagctcaga gtgccttcca
     5341 gatggcatac caggcatggg taaccaatgc ccagacagtg ctgaggcagc gtcgggagcg
     5401 ggcacggcag gagcgggcag agcagctggc ttctggaggt gacttgaacc cagatgtgga
     5461 accagtagat gtcccagaag atgagatggc aggccgtagc cacatgatgc agcgtgtgct
     5521 aagcaccatg cagttcctgt gggtgctggg ccaggccacg gtagacgggc tgacgcgctg
     5581 gctgcgtgca ttcacgaagc accaccgcac catgagcgat gtgctgtgcg cagagcgcta
     5641 cctgctcacc caggagcttc ttcgggttgg agaggtacgc cgaggtgtgc tggaccagct
     5701 ttatgtgggt gaagatgagg ccacattgtc aggtcccgtg gagacccggg atggacccag
     5761 cacagcctca agtgggctgg gagccgaaga gcctttgagt agcatgacag acgacaccag
     5821 cagccccctg agcacaggct ataacacccg cagtggcagt gaggagattg tcaccgacgc
     5881 tggggacctc caggctggga cctccctgca cggctcccaa gagcttttag ccaatgctcg
     5941 tacccggatg cgcacggcca gcgagctgct actggatagg cgcctgcata tccctgagct
     6001 ggaggaggcc gagcggtttg aggcacagca gggccggact ctgcggctgc tcagggctgg
     6061 gtaccagtgc gtggcggcac actcggagct gctctgttac ttcatcatca tccttaacca
     6121 catggtgaca gcctcggctg cctccctggt gctgcccgtg cttgtgttcc tgtgggccat
     6181 gctgaccatc ccgaggccta gcaagcgctt ttggatgaca gctatcgtct tcactgaggt
     6241 catggtggtc accaaatacc tgttccagtt cggcttcttc ccctggaaca gctacgttgt
     6301 gctgcggcgc tatgagaaca agccctactt ccctccgcga atcctgggcc ttgagaaaac
     6361 ggacagctac atcaagtatg acctggtgca gctcatggcc ctcttcttcc accgctcgca
     6421 gctactgtgt tatggcctct gggaccatga ggaggatcgc tatcccaagg accattgcag
     6481 gagtagtgtg aaggaccggg aggccaagga agagccagaa gctaagctgg aatcgcagtc
     6541 tgagacaggc actgggcatc ccaaggagcc agtgttggcc ggtactccca gggaccacat
     6601 ccaagggaaa ggaagtatta gatccaagga tgttatccaa gatcccccag aggaccttaa
     6661 gccccggcac acgaggcaca tcagcatacg cttcaggagg cgcaaggaga ctccaggacc
     6721 caaaggaaca gcagtcatgg agactgagca cgaggaggga gaaggaaaag aaactacaga
     6781 gagaaagagg ccgcgtcaca ctcaagaaaa atcgaagttt cgggagagaa tgaaggcagc
     6841 tgggcgccgg ctgcagagct tctgtgtgtc actggcccag agcttctacc aacccttgca
     6901 gcgtttcttc catgacattc tgcacacaaa gtaccgggcg gccaccgacg tctacgccct
     6961 catgttcctg gccgatattg tcgacatcat catcatcatc tttggttttt gggcttttgg
     7021 gaagcactct gcagccacag acattgcatc ctcgctgtca gatgaccagg tgccacaggc
     7081 cttcctgttc atgctgctgg tccagtttgg caccatggtc atcgaccgtg ccctctacct
     7141 gcgcaagact gtcctgggaa agctggcctt tcaggtggtc ctggtggtgg cgattcacat
     7201 ctggatgttc tttatcttac cggctgtcac tgagaggatg ttcagccaga atgcggtggc
     7261 acagctgtgg tacttcgtca agtgcattta ctttgccctg tccgcctacc agatccgctg
     7321 tggctacccc acccgtatct tgggcaactt cctcaccaag aaatacaacc atctaaacct
     7381 cttccttttc caggggttcc gtctagtgcc gttcctggtg gagctgcggg ccgtcatgga
     7441 ctgggtgtgg accgacacca cgctgtccct gtccaactgg atgtgtgtgg aagacatcta
     7501 tgccaacatc tttatcatca agtgcagccg agagacagag aagaaatacc cccagcccaa
     7561 ggggcagaag aagaagaaaa ttgtcaagta tggtatggga ggcctcatta tcctcttcct
     7621 catcgccatc atctggttcc ctctgctctt catgtcactg atccgctctg tggtcggggt
     7681 cgtcaaccag cccattgatg tcaccgtcac cctcaagcta ggcggctatg agccactgtt
     7741 caccatgagc gcccagcagc catccattgt gccattcaca ccccaggcct acgaggagct
     7801 gtcccagcag tttgacccct atccactagc catgcagttc attagccagt acagtcctga
     7861 ggacatcgtc actgcacaga tcgagggcag ctcgggggcg ctgtggcgca tcagcccacc
     7921 cagccgagcc cagatgaagc aggagctgta caacggcaca gccgacatta cactgcgctt
     7981 tacctggaat ttccaaaggg acctggccaa gggtggcact gtggagtata ctaatgagaa
     8041 gcacaccttg gagctggccc ccaacagtac ggcacgaagg cagctggccc aactgctcga
     8101 gggcagacct gaccagtcag tggtcattcc ccatctcttc cccaagtaca ttcgtgctcc
     8161 caatgggcct gaagccaacc ctgtgaagca gctgcagcca gatgaggaag aggactacct
     8221 tggtgtgcgc atccagctgc ggagggagca agtgggcaca ggggcctctg gggagcaagc
     8281 gggcaccaag gcctccgact tcctcgagtg gtgggtcatc gagctgcagg actgcaaggc
     8341 tgactgcaac ctgctgccca tggtcatctt cagtgacaag gtcagcccac ctagcctggg
     8401 cttcctggcc ggctacggga ttgtggggct gtatgtctcc atcgtgctgg tggttggcaa
     8461 gtttgtgcgg ggcttcttca gcgagatctc tcactccatc atgttcgagg aactgccgtg
     8521 tgtggaccgc atcctcaagc tgtgccagga catcttcttg gtgcgcgaga cccgggagct
     8581 ggagctggag gaggagctat acgccaagct catcttcctg taccgatctc cagagaccat
     8641 gattaagtgg acacgtgaga gggaggacgt gggtggcgga gggagcgaag gcggcggatc
     8701 cggagggcct ggctctggcg gagaggggag cgctggaggc gggtcagccg gtggtgggag
     8761 tgatctgcct gatgaccact acctgagcac acagaccatc ctctccaagg accttaacta
     8821 agaattcaga tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg
     8881 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     8941 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     9001 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggta
     9061 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     9121 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     9181 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     9241 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     9301 ctggggcctc ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     9361 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac
     9421 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     9481 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     9541 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     9601 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     9661 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     9721 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     9781 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     9841 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     9901 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     9961 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
    10021 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
    10081 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aaaccggtgc
    10141 tcgaggtacc gatgaatagc taaggtcgag gccgcaggta agtatcaagg ttacaagaca
    10201 ggtttaagga gaccaataga aactgggctt gtcgagacag agaagactct tgcgtttctg
    10261 ataggcacct attggtctta ctgacatcca ctttgccttt ctctccacag gtgtcgacaa
    10321 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
    10381 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
    10441 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
    10501 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
    10561 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
    10621 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
    10681 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
    10741 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
    10801 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
    10861 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc
    10921 tcggtacgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
    10981 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
    11041 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
    11101 gacagcaagg gggaggattg ggaagacaat agcccagctt ttgttccctt tagtgagggt
    11161 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
    11221 taattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc
    11281 ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg
    11341 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
    11401 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
    11461 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
    11521 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
    11581 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
    11641 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
    11701 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc ggccggccgc
    11761 cggcgtcgac gcgcgcggcc gcggcgcgcc gttgctggcg tttttccata ggctccgccc
    11821 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    11881 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    11941 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    12001 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    12061 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    12121 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    12181 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    12241 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    12301 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    12361 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    12421 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    12481 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    12541 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    12601 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    12661 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    12721 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    12781 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    12841 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    12901 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    12961 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    13021 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    13081 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    13141 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    13201 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    13261 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    13321 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    13381 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    13441 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    13501 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    13561 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    13621 tctacgcgcg cgccggcggc gcgccgtcga cggccggccg cggccgc
//
