LOCUS       sequence-249445-       13667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PPI (BiFC/TriFC) assay vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13667)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 13667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-504    N-Tag GFP[1] L32 Piezo1 mCherry
FEATURES             Location/Qualifiers
     source          1..13667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        153..532
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        533..736
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     687..707
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     733..757
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          872..1004
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     regulatory      1019..1028
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(8835..9409,9410..9412,9413..9421)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9002..9019)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9229..9248
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9422..10132
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9567..9585)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9835..9854
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     intron          10178..10310
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     misc_feature    10319..10907
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10372..10392)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10790..10801)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10934..10951)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10940..11051
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(11147..11167)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11147..11165)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11186..11202)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11186..11202)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11199..11221)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     complement(11304..11323)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    11369..11424
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(11423..11442)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(11803..12391)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11883..11902)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12562..13353,13354..13422))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13185..13204
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13423..13527)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13595..13613
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcctgcctcc aggcctcaat attggccatt agccatatta ttcattggtt atatagcata
       61 aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta
      121 tattggctca tgtccaatat gaccgccatg ttggcattga ttattgacta gttattaata
      181 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      241 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      301 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      361 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtccgccccc
      421 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttacg
      481 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      541 gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      601 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      661 atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt
      721 ctatataagc agagctcgtt tagtgaaccg tcagatcact agaagcttta ttgcggtagt
      781 ttatcacagt taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag
      841 ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta
      901 aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc
      961 acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccaggc
     1021 caccatggat ctgcctgatg accactacct gagcacacag accatcctct ccaaggacct
     1081 taacgacgtg ggtggcggag ggagcgaagg cggcggatcc ggagggcctg gctctggcgg
     1141 agaggggagc gctggaggcg ggtcagccgg tggtgggagt gagccgcacg tgctgggcgc
     1201 cgggctctac tggctgttgc tgccctgcac gctcctggcg gcctccctgt tacgcttcaa
     1261 tgctctctcg ctggtctatt tgctgtttct actgctgctg ccctggcttc caggcccctc
     1321 aagacacagc ataccaggtc acacaggtcg cctgctccgt gcactactct gcctcagcct
     1381 cctcttcctg gtggcccacc ttgcctttca gatatgccta cacaccgtgc ctcacctgga
     1441 ccagtttctg ggacaaaacg gtagcctttg ggtgaaggtg tctcaacaca taggggttac
     1501 gaggctggac ctgaaggaca tctttaacac caccaggctg gtagcacctg acctgggagt
     1561 gctgctggcg tcctcccttt gccttggcct ctgtggacgc ctcacgagga aagccgggca
     1621 gagtcggcgc acccaggagc tgcaggatga tgacgatgac gatgatgacg acgatgaaga
     1681 catagatgct gccccagccg tggggctgaa gggagcccct gccctggcaa ccaaacgcag
     1741 gctgtggctg gcctcccgct tccgggtcac ggcccactgg ctgctgatga cctctggacg
     1801 gacgctggtc attgtgttgc tggccctggc aggcatagcc cacccttcgg ccttctccag
     1861 catctacctg gtggtgttcc tggccatctg cacctggtgg tcctgtcact ttcctctcag
     1921 ccccctgggc tttaacaccc tctgtgtcat ggtgagctgt tttggtgccg gccatctcat
     1981 ttgcctatac tgctatcaga caccatttat ccaggacatg ttaccccctg ggaacatctg
     2041 ggccaggcta tttggtctca agaacttcgt agacctccct aactactcca gccccaacgc
     2101 cctggtactc aacactaagc acgcctggcc catctatgtg agtcccggaa tcctgctgct
     2161 gctatattac acagccacct ctctcctgaa gctccacaag agctgtccct cagagctgag
     2221 gaaggagaca cccagggagg atgaggagca tgagctggaa ctggaccacc tggagccaga
     2281 gccccaggct agggacgcca cccagggtga gatgcccatg accacggaac ctgaccttga
     2341 caactgcacc gtgcatgtac taaccagcca gagccctgtc cgccagcgtc cagttcgccc
     2401 caggctggct gagctgaaag agatgtcacc gctacatggc ctgggccacc tcatcatgga
     2461 ccagagctat gtgtgtgccc tgattgccat gatggtgtgg agcatcatgt accacagttg
     2521 gctgaccttc gtcctgctgc tctgggcctg cctcatctgg acggtgcgta gccgtcacca
     2581 gctggctatg ctctgctcgc cctgcatcct gctgtatggg ctgacgctct gctgcctgag
     2641 gtatgtgtgg gccatggaac ttcctgagct gcccaccacc ctgggccctg tcagcctgca
     2701 ccagttggga ctggaacaca cacgctaccc ttgcctggac ctcggtgcca tgctcctcta
     2761 tctgctcaca ttctggctcc ttctgcgtca gttcgtgaag gagaagctgc tgaagaagca
     2821 aaaggtgcct gcggcgctgc tggaggtcac ggtggccgac actgagccca cacagaccca
     2881 gacgctgctg cggagcctgg gggagctggt caccggcatc tacgtcaaat actggatcta
     2941 tgtgtgcgcc ggcatgttca ttgtggtcag cttcgccggc cgcctggtgg tctacaaaat
     3001 cgtctacatg ttcctcttcc tgctgtgcct caccttgttc caggtctact acaccctgtg
     3061 gaggaagctg ctacgtgtct tctggtggct cgtggtggcc tatacaatgc tcgtgctcat
     3121 cgctgtgtac accttccagt tccaggactt ccccacctat tggcgcaacc tcacgggctt
     3181 cacagacgag cagttgggcg acctgggcct ggagcagttc agtgtgtcgg agctcttttc
     3241 cagtatcctc atccctggct tcttcctgct ggcctgcatc ctgcagctgc actacttcca
     3301 cagaccgttc atgcagctca ctgacctgga gcacgtgccg ccaccaggca cccgccaccc
     3361 tcgatgggct cacaggcagg atgcagtgag cgaggcccct ctgcttgagc atcaggagga
     3421 agaggaagtc ttcagggaag atgggcagag catggatggg ccccaccagg ccacacaggt
     3481 ccctgagggt acggccagca agtggggcct ggtggctgac cggctgctgg acctagcggc
     3541 cagcttctcg gctgtcctca cccgaatcca ggtgttcgtt cggcgcttgc tagaacttca
     3601 cgtcttcaag ctggtggccc tctacactgt ctgggtggcc ctgaaagaag tgtctgtgat
     3661 gaacctgctg ctggtggtgc tatgggcctt cgccttgccc tatccgcgct tccggcccat
     3721 ggcttcctgc ctgtccaccg tgtggacctg tatcatcatt gtatgcaaga tgctctatca
     3781 gctcaagatt gtcaacccgc atgagtactc cagcaactgc actgagccct tccccaacaa
     3841 taccaacttg cagcctttgg agatcaacca gtctttgctg taccgtggcc ctgttgaccc
     3901 tgccaactgg tttggggtgc ggaagggtta ccccaacttg ggctatatcc agaaccacct
     3961 gcagatcctt ctgttgctgg tgtttgaggc cgtggtgtac cggcgccaag agcactaccg
     4021 ccggcagcac cagcaggccc ctctgcccgc ccaggctgtg tgcgcagatg gcacccgcca
     4081 gaggttggac caggacctac ttagctgcct caagtatttc atcaacttct tcttctacaa
     4141 attcgggctg gagatctgct tcttgatggc cgtgaatgtg attgggcagc gtatgaactt
     4201 catggtgatc ttacacggtt gctggttggt ggccatcctt acacgccggc gccgtgaggc
     4261 catcgcccgc ctctggccta actactgtct gttcctcacg ctgttcctgc tgtaccagta
     4321 cctgctgtgt ttgggcatgc cccctgcact gtgcattgac tatccatggc gctggagcaa
     4381 ggccatcccc atgaattccg ccctcatcaa gtggctgtac ctacctgact tcttcagagc
     4441 ccccaactcc accaacctta tcagtgactt cctcctgctg ctttgcgcct cccagcagtg
     4501 gcaggtcttc tcagcggagc gaacggagga gtggcaacgc atggcgggca tcaacactga
     4561 ccacctggag cccctgcgtg gggagcccaa ccctataccc aacttcatcc actgcaggtc
     4621 ctatctggat atgctgaagg tggccgtctt ccgctacctg ttctggctgg tgctcgttgt
     4681 ggtgtttgtt gcgggggcca cccgcataag catcttcggg ctggggtacc tgctagcctg
     4741 cttctacctg ctgctgtttg gcactaccct gctgcagaag gacacgcgag cccagctcgt
     4801 gctgtgggac tgcctcatcc tctataatgt cactgtcatc atctctaaga atatgctgtc
     4861 gctcctgtcc tgtgtcttcg tggagcaaat gcagagcaac ttctgctggg tcatccagct
     4921 cttcagcctc gtgtgcacag tcaaaggcta ctatgatccc aaagagatga tgaccaggga
     4981 ccgggactgc ctgctgcctg tggaggaggc cgggatcatc tgggacagta tctgcttctt
     5041 cttcctgctc ttgcaacggc gcatctttct cagccactac ttcctgcatg tcagcgctga
     5101 cctgaaagcc acagccctgc aggcatccag gggctttgcc ctctacaatg cagccaacct
     5161 gaagagcatc aacttccatc gccagattga ggagaagtcc ctggcccagc tgaaaagaca
     5221 gatgaagcgc atccgtgcca aacaggagaa gtacaggcag agccaggcaa gtcgtggcca
     5281 actccagtcc aaagaccctc aggatcccag ccaggagcca gggcctgaca gcccaggggg
     5341 ctcctccccg ccacggagac agtggtggcg cccctggctg gaccacgcca cagtcatcca
     5401 ctctggcgac tacttcctgt ttgagtcaga tagcgaggag gaagaggagg ccctacctga
     5461 ggaccccagg cctgcagctc agagtgcctt ccagatggca taccaggcat gggtaaccaa
     5521 tgcccagaca gtgctgaggc agcgtcggga gcgggcacgg caggagcggg cagagcagct
     5581 ggcttctgga ggtgacttga acccagatgt ggaaccagta gatgtcccag aagatgagat
     5641 ggcaggccgt agccacatga tgcagcgtgt gctaagcacc atgcagttcc tgtgggtgct
     5701 gggccaggcc acggtagacg ggctgacgcg ctggctgcgt gcattcacga agcaccaccg
     5761 caccatgagc gatgtgctgt gcgcagagcg ctacctgctc acccaggagc ttcttcgggt
     5821 tggagaggta cgccgaggtg tgctggacca gctttatgtg ggtgaagatg aggccacatt
     5881 gtcaggtccc gtggagaccc gggatggacc cagcacagcc tcaagtgggc tgggagccga
     5941 agagcctttg agtagcatga cagacgacac cagcagcccc ctgagcacag gctataacac
     6001 ccgcagtggc agtgaggaga ttgtcaccga cgctggggac ctccaggctg ggacctccct
     6061 gcacggctcc caagagcttt tagccaatgc tcgtacccgg atgcgcacgg ccagcgagct
     6121 gctactggat aggcgcctgc atatccctga gctggaggag gccgagcggt ttgaggcaca
     6181 gcagggccgg actctgcggc tgctcagggc tgggtaccag tgcgtggcgg cacactcgga
     6241 gctgctctgt tacttcatca tcatccttaa ccacatggtg acagcctcgg ctgcctccct
     6301 ggtgctgccc gtgcttgtgt tcctgtgggc catgctgacc atcccgaggc ctagcaagcg
     6361 cttttggatg acagctatcg tcttcactga ggtcatggtg gtcaccaaat acctgttcca
     6421 gttcggcttc ttcccctgga acagctacgt tgtgctgcgg cgctatgaga acaagcccta
     6481 cttccctccg cgaatcctgg gccttgagaa aacggacagc tacatcaagt atgacctggt
     6541 gcagctcatg gccctcttct tccaccgctc gcagctactg tgttatggcc tctgggacca
     6601 tgaggaggat cgctatccca aggaccattg caggagtagt gtgaaggacc gggaggccaa
     6661 ggaagagcca gaagctaagc tggaatcgca gtctgagaca ggcactgggc atcccaagga
     6721 gccagtgttg gccggtactc ccagggacca catccaaggg aaaggaagta ttagatccaa
     6781 ggatgttatc caagatcccc cagaggacct taagccccgg cacacgaggc acatcagcat
     6841 acgcttcagg aggcgcaagg agactccagg acccaaagga acagcagtca tggagactga
     6901 gcacgaggag ggagaaggaa aagaaactac agagagaaag aggccgcgtc acactcaaga
     6961 aaaatcgaag tttcgggaga gaatgaaggc agctgggcgc cggctgcaga gcttctgtgt
     7021 gtcactggcc cagagcttct accaaccctt gcagcgtttc ttccatgaca ttctgcacac
     7081 aaagtaccgg gcggccaccg acgtctacgc cctcatgttc ctggccgata ttgtcgacat
     7141 catcatcatc atctttggtt tttgggcttt tgggaagcac tctgcagcca cagacattgc
     7201 atcctcgctg tcagatgacc aggtgccaca ggccttcctg ttcatgctgc tggtccagtt
     7261 tggcaccatg gtcatcgacc gtgccctcta cctgcgcaag actgtcctgg gaaagctggc
     7321 ctttcaggtg gtcctggtgg tggcgattca catctggatg ttctttatct taccggctgt
     7381 cactgagagg atgttcagcc agaatgcggt ggcacagctg tggtacttcg tcaagtgcat
     7441 ttactttgcc ctgtccgcct accagatccg ctgtggctac cccacccgta tcttgggcaa
     7501 cttcctcacc aagaaataca accatctaaa cctcttcctt ttccaggggt tccgtctagt
     7561 gccgttcctg gtggagctgc gggccgtcat ggactgggtg tggaccgaca ccacgctgtc
     7621 cctgtccaac tggatgtgtg tggaagacat ctatgccaac atctttatca tcaagtgcag
     7681 ccgagagaca gagaagaaat acccccagcc caaggggcag aagaagaaga aaattgtcaa
     7741 gtatggtatg ggaggcctca ttatcctctt cctcatcgcc atcatctggt tccctctgct
     7801 cttcatgtca ctgatccgct ctgtggtcgg ggtcgtcaac cagcccattg atgtcaccgt
     7861 caccctcaag ctaggcggct atgagccact gttcaccatg agcgcccagc agccatccat
     7921 tgtgccattc acaccccagg cctacgagga gctgtcccag cagtttgacc cctatccact
     7981 agccatgcag ttcattagcc agtacagtcc tgaggacatc gtcactgcac agatcgaggg
     8041 cagctcgggg gcgctgtggc gcatcagccc acccagccga gcccagatga agcaggagct
     8101 gtacaacggc acagccgaca ttacactgcg ctttacctgg aatttccaaa gggacctggc
     8161 caagggtggc actgtggagt atactaatga gaagcacacc ttggagctgg cccccaacag
     8221 tacggcacga aggcagctgg cccaactgct cgagggcaga cctgaccagt cagtggtcat
     8281 tccccatctc ttccccaagt acattcgtgc tcccaatggg cctgaagcca accctgtgaa
     8341 gcagctgcag ccagatgagg aagaggacta ccttggtgtg cgcatccagc tgcggaggga
     8401 gcaagtgggc acaggggcct ctggggagca agcgggcacc aaggcctccg acttcctcga
     8461 gtggtgggtc atcgagctgc aggactgcaa ggctgactgc aacctgctgc ccatggtcat
     8521 cttcagtgac aaggtcagcc cacctagcct gggcttcctg gccggctacg ggattgtggg
     8581 gctgtatgtc tccatcgtgc tggtggttgg caagtttgtg cggggcttct tcagcgagat
     8641 ctctcactcc atcatgttcg aggaactgcc gtgtgtggac cgcatcctca agctgtgcca
     8701 ggacatcttc ttggtgcgcg agacccggga gctggagctg gaggaggagc tatacgccaa
     8761 gctcatcttc ctgtaccgat ctccagagac catgattaag tggacacgtg agagggagta
     8821 agaattcaga tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg
     8881 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     8941 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     9001 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggta
     9061 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     9121 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     9181 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     9241 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     9301 ctggggcctc ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     9361 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac
     9421 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     9481 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     9541 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     9601 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     9661 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     9721 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     9781 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     9841 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     9901 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     9961 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
    10021 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
    10081 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt aaaccggtgc
    10141 tcgaggtacc gatgaatagc taaggtcgag gccgcaggta agtatcaagg ttacaagaca
    10201 ggtttaagga gaccaataga aactgggctt gtcgagacag agaagactct tgcgtttctg
    10261 ataggcacct attggtctta ctgacatcca ctttgccttt ctctccacag gtgtcgacaa
    10321 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
    10381 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
    10441 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
    10501 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
    10561 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
    10621 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
    10681 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
    10741 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
    10801 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
    10861 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc
    10921 tcggtacgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
    10981 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
    11041 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
    11101 gacagcaagg gggaggattg ggaagacaat agcccagctt ttgttccctt tagtgagggt
    11161 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
    11221 taattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc
    11281 ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg
    11341 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
    11401 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
    11461 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
    11521 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
    11581 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
    11641 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
    11701 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc ggccggccgc
    11761 cggcgtcgac gcgcgcggcc gcggcgcgcc gttgctggcg tttttccata ggctccgccc
    11821 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    11881 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    11941 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    12001 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    12061 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    12121 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    12181 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    12241 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    12301 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    12361 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    12421 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    12481 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    12541 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    12601 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    12661 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    12721 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    12781 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    12841 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    12901 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    12961 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    13021 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    13081 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    13141 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    13201 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    13261 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    13321 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    13381 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    13441 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    13501 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    13561 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    13621 tctacgcgcg cgccggcggc gcgccgtcga cggccggccg cggccgc
//
