LOCUS       sequence-249561-        4340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4340)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-VALENTIA-002   Lyn-SuperNova//BsaI
FEATURES             Location/Qualifiers
     source          1..4340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..814
                     /label=chicken beta-actin promoter
     regulatory      834..843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1725..1846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1762..1781)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1816..1835
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      2647..2676
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      2698..3285
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3186..3205
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3297..3324)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(3348..4139,4140..4208))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3971..3990
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4209..4311)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(join(4311..4340,1..2))
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctttctgcta tggaggtcag gtatgattta aaggtctcat aggtcttgta ttcagtctcg
       61 cactttagac atggtcttct gctgcccgtg gtcctctatt cgtcggatgt tctgtcgatt
      121 cttcctgtct atctggactg gatctgatat catcgtcgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      661 cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc
      721 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      781 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgg gacgccacca
      841 tgggctgcat caagagcaag aggaaagacg tggaaaacgt cgagggcggc agcgaggtag
      901 gtccagctct gtttcagtcc gatatgacgt ttaagatctt catcgatggc gaagtgaacg
      961 gtcagaagtt caccatagtt gctgatggct cttccaagtt cccacatggc gacttcaatg
     1021 cccatgcagt gtgtgaaact gggaaactgc ctatgagctg gaaacccatt tgccatctga
     1081 ttcaatgggg agaaccgttc ttcgcgaggt atcctgatgg cattagccac tttgcccagg
     1141 agtgctttcc agaggggctg agtatcgacc gtactgtgcg cttcgagaat gacgggacaa
     1201 tgacctctca ccacacctac gagttggacg acacatgtgt ggtcagcagg atcacggtga
     1261 actgtgacgg ctttcagcca gacggaccta tcatgcgcga tcagctcgtt gacatccttc
     1321 cctcagaaac acacatgttt ccgcatggac ctaatgccgt cagacagaca gcaactattg
     1381 ggtttaccac tgcagatggc gggaagatga tgggacactt cgacagcaag atgaccttca
     1441 acggcagtag agccatagag attcctggac cccatttcgt gaccatcatc accaaacaga
     1501 cacgggacac atccgacaaa cgagatcacg tctgccaaag ggaagtcgct tacgctcact
     1561 ctgtaccccg gattacttca gccataggta gcgatgagga ttcaggctct gattaaggcg
     1621 ccaggtacat ctccaatacg gcgtcttgca tagagaacac gattagcaca ccggttaccc
     1681 acgtgatgcc cgaacggacc catgtcatgc cagagcgcac tggtaacttg tttattgcag
     1741 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     1801 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtcgtc
     1861 gaattcgagt tggcgcgcct gtcattctaa atctctcttt cagcctaaag ctttttcccc
     1921 gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat
     1981 cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg
     2041 gggagcgccg gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg
     2101 gacgtaatta catccctggg ggctttggga gggggctgtc cccgtgagct cccaggcgcg
     2161 cctgtcattc taaatctctc tttcagccta aagctttttc cccgtatccc cccaggtgtc
     2221 tgcaggctca aagagcagcg agaagcgttc agaggaaagc gatcccgtgc caccttcccc
     2281 gtgcccgggc tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggaccgga
     2341 gcggagcccc gggcggctcg ctgctgcccc ctagcggggg agggacgtaa ttacatccct
     2401 gggggctttg ggggggggct gtccccgtga gctcccaggc gcgccaactc ggtacctcac
     2461 ccgattctca tcatggagac acatcaggac atctccaata ccgccatgga aacgcaccaa
     2521 gatgcgactg ctcttctgcc agtgacggca ttcgtctgca agattaacgg catacagaaa
     2581 gaaacgatga gacccattca ggacgagcct cagactccag cgtaactgga ctgcaatcaa
     2641 ctcactggct caccttcacg ggtgggcctt tcttcggtag aaaatcaaag gatcttcttg
     2701 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2761 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaggtaac tggcttcagc
     2821 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2881 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2941 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3001 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3061 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3121 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3181 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3241 catcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgca
     3301 gaaaggccca cccgaaggtg agccaggtga ttacatttgg gccctcatta ccaatgctta
     3361 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3421 cccgtcgtgt agataactac gatgcgggag ggcttaccat ctggccccag tgctgcaatg
     3481 ataccgcgag aaccacgctc accggctcca gatttatcag caataaacca gccagccggg
     3541 agggccgagc gcagaagtga tcctgcaact ttatccgcct ccatccagtc tattaattgt
     3601 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     3661 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     3721 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     3781 ggtcctccga tcgttgccag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     3841 gcactgcata attctcttac tgtcatgcca tccgtgagat gcttttctgt gactggtgag
     3901 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     3961 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4021 cgttcttcgg ggcgtaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4081 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4141 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4201 atactcattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     4261 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc agtcaaaagc
     4321 ctccggtcgg aggcttttga
//
