LOCUS       sequence-249592-        5054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5054)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-VALENTIA-033   NLSNoLS-Eos//BsaI
FEATURES             Location/Qualifiers
     source          1..5054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..814
                     /label=chicken beta-actin promoter
     regulatory      834..843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2439..2560
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2476..2495)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2530..2549
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      3361..3390
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      3412..3999
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3900..3919
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4011..4038)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(4062..4853,4854..4922))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4685..4704
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4923..5025)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(join(5025..5054,1..2))
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctttctgcta tggaggtcag gtatgattta aaggtctcat aggtcttgta ttcagtctcg
       61 cactttagac atggtcttct gctgcccgtg gtcctctatt cgtcggatgt tctgtcgatt
      121 cttcctgtct atctggactg gatctgatat catcgtcgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      661 cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc
      721 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      781 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgg gacgccacca
      841 tggccgtgaa cgtgtactca accagcgtaa caagcgagaa tctgtcaagg cacgacatgt
      901 tggcttgggt gaacgattcc cttcatctga actacaccaa aatcgaacag ctgtgctctg
      961 gagctgccta ttgccagttt atggacatgc tctttcccgg atgtgtgcac cttcggaaag
     1021 tcaaattcca ggcaaaactg gaacatgagt atatccacaa ctttaaggtt ctgcaagcag
     1081 cttttaagaa gatgggcgtg gacaagatca tcccggtgga aaagctcgtg aaagggaagt
     1141 ttcaggacaa tttcgagttc attcagtggt tcaagaagtt ctttgacgcc aactatgatg
     1201 ggaaagatta caatccactg cttgccagac agggacagga tgtcgcaccg ccacccaatc
     1261 ctggcgacca gatcttcaac aagagcaaga aactgattgg gacagcggtt ccacaacgta
     1321 cttcccctac tggccccaag aacatgcaga cgtctggcag actgagcaat gttgctccac
     1381 cctgtatact ccgcaagaat ccacctagtg ctcgaaatgg cggtcatgag acagatgccc
     1441 agatactgga gctcaaccaa cagctggtag acctgaaact gaccgtcgac ggattggaga
     1501 aagagaggga cttctacttc tccaagttgc gggatataga gctcatttgc caggaacacg
     1561 agagcgaaaa ctctcccgtc atcagtggta tcatcggtat tctgtatgcc actgaagaag
     1621 ggtttgcacc tcctgaagat gacgaaattg aggagcacca acaggaggat caagacgagt
     1681 acctggatgg cgggtcaggc ggcagctcag cgatcaaacc cgacatgaag atcaacctcc
     1741 gaatggaagg caacgtaaac ggccatcact tcgtgattga tggcgatggc acagggaagc
     1801 catttgaggg gaaacaaagc atggatctgg aggtgaagga aggtggtcct ctgccttttg
     1861 ccttcgacat actgactaca gcctttcact atgggaatcg cgtgtttgtc gagtatcccg
     1921 accatataca ggattacttc aagcagagtt tcccgaaagg ctatagctgg gaaaggtccc
     1981 tcacatttga ggatggaggg atttgcattg caagaaacga catcaccatg gaaggagaca
     2041 ccttctacaa caaggttcgg ttccatggga caaatttccc cgctaatggt cctgtgatgc
     2101 agaagaaaac cctgaagtgg gaaccatcca ctgagaagat gtacgtgaga gatggagtac
     2161 tgacgggtga cattcacgct gcactgctgt tggagggcaa tgcccactat cggtgtgatt
     2221 ttcgcactac ctacaaggcc aaagagaaag gcgtcaaact ccctggatac caccttgtcg
     2281 atcattgcat cgagatcctt tctcacgaca aggactacaa caaggtgaag ctgtatgaac
     2341 atgccgttgc tcactctgga ttgccagaca atgcaaggcg ttcaggctaa taacagcttc
     2401 cggactctag aacatcccta caggtgatat cctcgggtaa cttgtttatt gcagcttata
     2461 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     2521 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt cgtcgaattc
     2581 gagttggcgc gcctgtcatt ctaaatctct ctttcagcct aaagcttttt ccccgtatcc
     2641 ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg
     2701 ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg cggggggagc
     2761 gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg gagggacgta
     2821 attacatccc tgggggcttt gggagggggc tgtccccgtg agctcccagg cgcgcctgtc
     2881 attctaaatc tctctttcag cctaaagctt tttccccgta tccccccagg tgtctgcagg
     2941 ctcaaagagc agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc
     3001 gggctgtccc cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag
     3061 ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc
     3121 tttggggggg ggctgtcccc gtgagctccc aggcgcgcca actcggtacc tcacccgatt
     3181 ctcatcatgg agacacatca ggacatctcc aataccgcca tggaaacgca ccaagatgcg
     3241 actgctcttc tgccagtgac ggcattcgtc tgcaagatta acggcataca gaaagaaacg
     3301 atgagaccca ttcaggacga gcctcagact ccagcgtaac tggactgcaa tcaactcact
     3361 ggctcacctt cacgggtggg cctttcttcg gtagaaaatc aaaggatctt cttgagatcc
     3421 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3481 ttgtttgccg gatcaagagc taccaactct ttttccgagg taactggctt cagcagagcg
     3541 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3601 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3661 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3721 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3781 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3841 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3901 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcatcga
     3961 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcagaaagg
     4021 cccacccgaa ggtgagccag gtgattacat ttgggccctc attaccaatg cttaatcagt
     4081 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     4141 gtgtagataa ctacgatgcg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     4201 cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc cgggagggcc
     4261 gagcgcagaa gtgatcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     4321 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     4381 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     4441 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     4501 ccgatcgttg ccagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     4561 cataattctc ttactgtcat gccatccgtg agatgctttt ctgtgactgg tgagtactca
     4621 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     4681 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     4741 tcggggcgta aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     4801 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     4861 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     4921 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     4981 cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcagtcaa aagcctccgg
     5041 tcggaggctt ttga
//
