LOCUS       sequence-249606-        2466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2466)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-EMMI-026   mCherry(I-J)//Esp3I
FEATURES             Location/Qualifiers
     source          1..2466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(15..44)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             110..817
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(252..270)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     520..539
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      859..890
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        891..993
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             994..1809
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1067..1086)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1833..1860
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1872..2459)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1952..1971)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttgatttt ctaccgaaga aaggcccacc cgtgaaggtg agccagtgag ttgattgcag
       61 tccagttacg ctggagtctg aggctcgtcc tgaatgcgtc tctgcgtccg tgagcaaggg
      121 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
      181 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
      241 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
      301 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
      361 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
      421 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
      481 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
      541 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
      601 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
      661 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
      721 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
      781 ccactccacc ggcggcatgg acgagctgta caagtaatgc tagagacgtt taaatcatac
      841 ctgacctcca tagcagaaag tcaaaagcct ccgaccggag gcttttgact tgatcggcac
      901 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
      961 ttatcgagat tttcaggagc taaggaagct aaaatgagcc atattcaacg ggaaacgtct
     1021 tgctcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1081 cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg
     1141 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1201 gtcaggctaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     1261 actcctgatg atgcatggtt actcaccact gcgatcccag ggaaaacagc attccaggta
     1321 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     1381 cggttgcatt cgattcctgt ttgtaattgt ccttttaacg gcgatcgcgt atttcgcctc
     1441 gctcaggcgc aatcacgaat gaataacggt ttggttggtg cgagtgattt tgatgacgag
     1501 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataagctttt gccattctca
     1561 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     1621 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     1681 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     1741 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcacttgat gctcgatgag
     1801 tttttctaat gagggcccaa atgtaatcac ctggctcacc ttcgggtggg cctttctgcg
     1861 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgatgctca
     1921 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1981 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2041 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2101 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2161 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2221 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2281 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2341 aagccagtta cctcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2401 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2461 aagatc
//
