LOCUS       sequence-249607-        2462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2462)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-EMMI-027   mCherry(S-T)//Esp3I
FEATURES             Location/Qualifiers
     source          1..2462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             65..769
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(207..225)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     475..494
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      811..842
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        843..945
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             946..1761
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1019..1038)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1785..1812
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1824..2411)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1904..1923)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2433..2462)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa tgcgtctctg
       61 tgcagtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
      121 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
      181 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
      241 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
      301 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
      361 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
      421 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
      481 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
      541 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
      601 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
      661 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
      721 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaaga gcgagagacg
      781 tttaaatcat acctgacctc catagcagaa agtcaaaagc ctccgaccgg aggcttttga
      841 cttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
      901 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgag ccatattcaa
      961 cgggaaacgt cttgctcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     1021 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg
     1081 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     1141 acagatgaga tggtcaggct aaactggctg acggaattta tgcctcttcc gaccatcaag
     1201 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc agggaaaaca
     1261 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     1321 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cggcgatcgc
     1381 gtatttcgcc tcgctcaggc gcaatcacga atgaataacg gtttggttgg tgcgagtgat
     1441 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataagctt
     1501 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     1561 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     1621 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     1681 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcacttg
     1741 atgctcgatg agtttttcta atgagggccc aaatgtaatc acctggctca ccttcgggtg
     1801 ggcctttctg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1861 aatcgatgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1921 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1981 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2041 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2101 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2161 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2221 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2281 tgcgctctgc tgaagccagt tacctcggaa aaagagttgg tagctcttga tccggcaaac
     2341 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2401 aaggatctca agaagatcct ttgattttct accgaagaaa ggcccacccg tgaaggtgag
     2461 cc
//
