LOCUS       sequence-249610-        3155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3155)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-EMMI-030   SuperNovaGreen(G-H)//Esp3I
FEATURES             Location/Qualifiers
     source          1..3155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    773..789
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(797..803,804..821,822..827))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    842..863
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(980..997)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1151..1739)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1231..1250)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2048..2842
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2102..2121)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2712..2731
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2916..2934
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2972..2994)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3094..3113
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgtctcgcag cgaggtaggt ccagctctgt ttcagtccga tatgacgttt aagatcttca
       61 tcgatggcga agtgaacggt cagaagttca ccatagttgc tgatggctct tccaagttcc
      121 cacatggcga cttcaatgcc catgcagtgt gtgaaactgg gaaactgcct atgagctgga
      181 aacccatttg ccatctgatt caatggggag aaccgttctt cgcgaggtat cctgatggca
      241 ttagccactt tgcccaggag tgctttccag aggggctgag tatcgaccgt actgtgcgct
      301 tcgagaatga cgggacaatg acctctcacc acacctacga gttggacgac acatgtgtgg
      361 tcagcaggat cacggtgaac tgtgacggct ttcagccaga cggacctatc atgcgcgatc
      421 agctcgttga catccttccc tcagaaacac acatgtttcc gcatggacct aatgccgtca
      481 gacagacagc aactattggg tttaccactg cagatggcgg gaagatgatg ggacacttcg
      541 acagcaagat gaccttcaac ggcagtagag ccatagagat tcctggaccc catttcgtga
      601 ccatcatcac caaacagaca cgggacacat ccgacaaacg agatcacgtc tgccaaaggg
      661 aagtcgctta cgctcactct gtaccccgga ttacttcagc cataggtagc gatgaggatt
      721 caggccgaga cgacctgcta gcgctcgctt ggatccgaat tcaaacctga aattgttatc
      781 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
      841 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
      901 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
      961 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1021 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1081 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1141 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1201 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1261 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1321 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1381 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1441 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1501 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1561 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1621 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1681 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1741 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1801 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1861 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc cggaattgcc
     1921 agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt
     1981 gccgccaagg atctgatggc gcaggggatc aagatctgat caagagacag gatgaggatc
     2041 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     2101 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     2161 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     2221 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     2281 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     2341 ggggcaggat ctcctgtcat cccaccttgc tcctgccgag aaagtatcca tcatggctga
     2401 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     2461 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     2521 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     2581 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     2641 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     2701 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     2761 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     2821 ccttcttgac gagttcttct gatccgcgca catttccccg aaaagtgcca cctgacgtct
     2881 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     2941 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     3001 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     3061 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     3121 aaacccaatt gggtaccgag ctccctgcag gaagc
//
