LOCUS       sequence-249649-       12014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a wheat codon optimized Cas9 module in forward direction for
            golden gate cloning of genome editing binary plasmids..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12014)
  TITLE     Genome editing in wheat
REFERENCE   2  (bases 1 to 12014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL11086_GG_Wheat_live_Cas9_rev
FEATURES             Location/Qualifiers
     source          1..12014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(256..969,970..972,973..975))
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             complement(1447..1503)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(1519..1539)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(1546..1566)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(1579..1605)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(1585..1611)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(1615..5718)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(1615..5718)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     regulatory      5715..5724
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5773..5793)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      5796..5805
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7717..7741
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(7897..9045)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(9381..9969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9461..9480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10140..10931,10932..11000))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10763..10782
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11001..11105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11192..11823
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    11915..11939
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caagcaagaa ttcaagctta gcgtatattt tgttttctat cgcgtattaa
       61 atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt catgcattac
      121 atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga
      181 ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaaca ctagtgatat
      241 ctgcggccgc agttatcact tgtagagctc atccatccca tgagtgatgc ctgcagccgt
      301 tacaaactcc agaagcacca tgtgatccct cttttcgtta gggtccttag acagaacgga
      361 ttgggttgac aggtagtgat tgtcgggcag aagcactggg ccatcgccta tcggcgtgtt
      421 ctgctggtag tggtcagcca gttggacact accatcctcc acattatgcc ggattttgaa
      481 gttcgcctta atgccgttct tctgcttgtc ggcagtaatg tagacgttgt gagaattgaa
      541 gttgtactcg agcttatgcc ccagtatgtt gccgtcctct ttgaagtcga tccctttgag
      601 ttcgatcctg ttcaccagtg tatctccctc gaacttgacc tccgcacgtg tcttgtacgt
      661 tccgtcgtcc ttaaagctga tggtgcgttc ttggacatac ccctcaggca tggctgactt
      721 gaagaagtca tggcgtttca tgtggtccgg atacctcgaa aagcactgaa caccatatgt
      781 gagagtggtg accaacgttg gccatggaac cggcagtttc ccagtggtac agatgaactt
      841 gagggtaagc ttgccattgg tggcatcacc ttcaccttcg cctctcacgc tgaacttgtg
      901 gccatttacg tcgccatcca actcgaccaa aatgggcaca actcctgtaa agagctcctc
      961 tcctttggac accatgaaag aggcgccctg agcagaactc gtaaacttcg taaccgcctt
     1021 agtcccttca gacacagcat gtttcgccaa ctcaccgggc aacacaagtc taaccgcagt
     1081 ctgaatctcc ctggaagtaa tcgtcggctt cttgttgtac ctcgccagct tcgaagattc
     1141 accagcgagc ttctcaaaaa tatcgttgat gaaactgttc ataattccca tcgctttgct
     1201 tgagattccg atgtctggat gaacttgctt caacaccttg aagatgtaga tcttgtatgt
     1261 ctcaacgttc ttctttgatc tcttcttctt cttgtctccg gcgacggctg gttccttggg
     1321 gagtttcttt ccggcttttg gtttcttctc tgccgctgct gctggttcga ctgccggagt
     1381 tttctctgcc ggtttcttct ccgctggttt cttatctgcc ttcgccatgg acgttgagcc
     1441 tggagctggg ccaggattct cctccacatc accagcttgc ttgagcaggg aaaagttcgt
     1501 ggctccggat ccgccgtcga ctttgcgctt cttcttggga tcctccactt tccgcttctt
     1561 cttaggggaa ccagacccaa gggaggcgta atctggcacg tcatacggat aggcatctcc
     1621 tcctagttgc gacaagtcga tcctcgtttc gtacaggcct gtaatagact ggtgaattag
     1681 ggtggcatcg agtacttctt tggtgctggt atagcgcttc ctatcaatcg tcgtgtcaaa
     1741 atatttgaac gccgcaggag cgccaaggtt cgtcaacgtg aacaagtgta tgatgttttc
     1801 tgcctgctcc cggatgggtt tgtcccgatg cttgttgtag gcggacaaca ccttgtcaag
     1861 gttagcgtca gcaagaatca ccctcttcga gaactcactg atctgttcga tgatctcatc
     1921 gaggtagtgc ttgtgttgct ccacaaacag ctgcttttgc tcattgtcct ctggggaacc
     1981 cttaagtttc tcatagtgtg aggcgagata caggaagttg acgtacttgg atgggagtgc
     2041 cagctcgttc cccttttgca actcgcctgc gctggcaagc atcctctttc tcccattctc
     2101 cagctcgaac aagctgtatt tcggcaactt tatgatgaga tctttcttga cttccttgta
     2161 tcctttcgcc tccaagaaat caatgggatt cttctcaaag ctggaacgct ccataatggt
     2221 aatcccgagc aactccttta cgctcttcag cttcttcgac ttcccctttt ccaccttggc
     2281 aacaaccagc acggagtacg ccactgtagg actgtcaaag ccaccgtact tctttggatc
     2341 ccagtctttc ttacgggcta taagcttatc gctattgcgc ttcggaagga ttgactcttt
     2401 cgagaagccg ccggtttgga cttccgtttt cttgacgatg ttgacttgtg gcatgcttag
     2461 caccttccgg acagtagcaa agtctctccc cttgtcccac actatctcac cagtttctcc
     2521 gttagtctct atcagcggac gttttctgat ctctccattg gccagggtga tctcagtctt
     2581 gaagaagttc attatgttgc tgtaaaagaa gtacttggcg gtggccttgc cgatttcctg
     2641 ctcactcttc gcaatcatct tccttacgtc ataaaccttg tagtcaccgt agacgaactc
     2701 cgactcaagc ttgggatatt tcttgatgag tgcagtgcca accacagcat tgagataggc
     2761 atcatgggcg tgatggtagt tgttgatctc acggacctta tagaactgga agtccttccg
     2821 aaagtcggac accagtttag atttcagagt aataactttg acttcccgga ttagcttgtc
     2881 attctcatcg tattttgtgt tcatgcgtga gtccaggatc tgtgcaacgt gcttggtgat
     2941 ttgccgtgtc tcaaccagtt ggcgcttgat aaatccggcc ttatctagct cactgaggcc
     3001 gcctctctcc gccttggtaa ggttgtcgaa cttcctttgt gtaatgagct ttgcattgag
     3061 gagttgcctc cagtagttct tcatcttctt gacgacttcc tcagaaggca cgttgtctga
     3121 cttcccgcgg ttcttgtcgg acctggtcag caccttgttg tcgatagagt cgtctttgag
     3181 gaagctttgt ggtactatat gatcgacatc gtagtcagat aggcgattga tgtcgagctc
     3241 ctggtccacg tacatatcgc gtccgttttg gaggtagtag aggtagagct tctcgttttg
     3301 cagttgggtg ttttcgaccg gatgctcctt gagaatttgt gatccgagct ccttgatgcc
     3361 ttcttcgatt ctcttcatcc tttccctgga gttcttctgc cccttctgtg tggtttggtt
     3421 ctcccgagcc atctcaatca cgatattctc gggcttatgt ctacccatca ctttgaccag
     3481 ctcatcgaca accttcactg tttgaagtat acccttcttg atggctggag atccggccaa
     3541 attcgcgata tgctcgtgga gcgaatcgcc ttggcctgaa acctgagcct tctgaatgtc
     3601 ctccttgaag gtcaaggagt catcgtggat tagctgcata aagttcctgt ttgcgaaccc
     3661 atcggacttg aggaagtcga ggatggtctt gcccgactgc ttatcgcgaa tgccattgat
     3721 cagcttcctg ctcagcctgc cccaacctgt gtagcgcctg cgcttcagct gcttcatcac
     3781 tttgtcgtca aagaggtgcg catacgtctt gagcctctct tcgatcatct cgcggtcctc
     3841 gaagagagta agcgtcagaa cgatgtcctc caagatgtcc tcgttctctt cgttatcgag
     3901 gaagtcttta tccttaatga tcttgagcaa gtcgtggtac gttcccagcg aggcgttgaa
     3961 gcgatcctca acgccgctta tctccacaga gtcaaagcac tcgattttct taaagtagtc
     4021 ctcctttagc tgcttgactg tgaccttgcg attggtcttg aaaaggagat ccactatggc
     4081 cttcttctgc tctcctgaca gaaaggccgg tttcctcata ccctcagtaa cgtacttgac
     4141 cttggtcagc tcgttgtaga cggtgaagta ctcgtacagt agggaatgtt tggggaggac
     4201 cttctcgttt ggcagattct tgtcgaagtt ggtcatcctc tcgatgaagc tttgggcaga
     4261 agcgcccttg tccaccacct cctcgaagtt ccatggggta atcgtttcct ccgattttct
     4321 agtcatccac gcgaacctag agttgcccct tgccaatggg cccacatagt aggggattct
     4381 gaatgtgagg atcttctcga ttttctcccg gttgtctttg aggaatgggt aaaagtcctc
     4441 ttgcctacga agtatggcgt gaagctcgcc cagatggatt tggtgtggaa tgcttccatt
     4501 gtcgaaggta cgctgcttgc ggagtagatc ctcgcgattg agcttaacca ggagctcttc
     4561 agtgccatcc atcttctcga gaatgggctt gataaacttg tagaactcct cctgtgacgc
     4621 gccaccgtcg atgtacccag catacccgtt ctttgattga tcgaagaaga tctctttgta
     4681 cttctccggc agctgttgtc tgacgagagc cttgaggagc gtgagatcct ggtgatgttc
     4741 gtcgtaccgt ttgatcatgg atgccgaaag aggagccttg gtaatctcag tgttaacgcg
     4801 cagaatatcg gacaagagga ttgcatctga caagttcttc gctgcaagga aaaggtccgc
     4861 atactggtcg ccgatttggg caagcagatt gtcgagatcg tcgtcgtacg tgtctttgga
     4921 cagttggagc ttggcatcct cggccaggtc aaagttagac ttgaagttag gggtgagccc
     4981 tagactaagg gcaataaggt tgccaaacaa tccgttcttc ttttcgcctg gcagttgggc
     5041 tatcagattt tcgagcctcc ggcttttcga cagccgagca ctcaagattg ccttcgcatc
     5101 aactcctgac gcgttgatgg ggttctcttc aaagagctga ttgtatgtct ggaccagttg
     5161 gatgaagagt ttgtcaacat cggaattgtc cggattaagg tcgccttcga tgaggaagtg
     5221 cccacggaac ttaatcatat gggccagggc caaatagatg aggcgcaaat cagccttgtc
     5281 agtgctatct acgagcttct tcctcaggtg atagatggta ggatacttct cgtggtatgc
     5341 gacttcgtcg acaatgttcc cgaatatcgg atggcgttcg tgcttcttgt cctcttctac
     5401 gaggaagctt tcctcgagcc gatggaagaa ggagtcatcc actttcgcca tttcgttgga
     5461 aaagatctct tgtaggtagc agatcctgtt ctttctacgc gtatagcgcc ttcttgctgt
     5521 gcgcttgagg cgggtcgctt cggctgtctc cccggaatca aagagcaacg cacctattag
     5581 gttcttcttg atggagtgcc tgtcggtgtt gcccagcact ttaaatttct tcgatggcac
     5641 cttgtactcg tcagtgatga cagcccaacc cacactattc gttcctatat ccaacccgat
     5701 agagtacttc ttgtccatgg tggcaatgcc aggacccctg taccgaaggc tgctaatccg
     5761 acacacccta cccaccttcc tcttcttctt cggccccatg gtggcacgcg tgagaaatct
     5821 cgagagctcc agattctccc cgggaattcc agatcctcta gagtcgacct gcagaagtaa
     5881 caccaaacaa cagggtgagc atcgacaaaa gaaacagtac caagcaaata aatagcgtat
     5941 gaaggcaggg ctaaaaaaat ccacatatag ctgctgcata tgccatcatc caagtatatc
     6001 aagatcaaaa taattataaa acatacttgt ttattataat agataggtac tcaaggttag
     6061 agcatatgaa tagatgctgc atatgccatc atgtatatgc atcagtaaaa cccacatcaa
     6121 catgtatacc tatcctagat cgatatttcc atccatctta aactcgtaac tatgaagatg
     6181 tatgacacac acatacagtt ccaaaattaa taaatacacc aggtagtttg aaacagtatt
     6241 ctactccgat ctagaacgaa tgaacgaccg cccaaccaca ccacatcatc acaaccaaga
     6301 caaaaaaaag catgaaaaga tgacccgaca aacaagtgca cggcatatat tgaaataaag
     6361 gaaaagggca aaccaaaccc tatgcaacga aacaaaaaaa atcatgaaat cgatcccgtc
     6421 tgcggaacgg ctagagccat cccaggattc cccaaagaga aacactggca agttagcaat
     6481 cagaacgtgt ctgacgtaca ggtcgcatcc gtgtacgaac gctagcagca cggatctaac
     6541 acaaacacgg atctaacaca aacatgaaca gaagtagaac taccgggccc taaccatgga
     6601 ccggaacgcc gatctagaga aggtagagag gggggggggg aggacgagcg gcgtaccttg
     6661 aagcggaggt gccgacgggt ggatttgggg gagatctggt tgtgtgtgtg tgcgctccga
     6721 acaacacgag gttggggaaa gagggtgtgg agggggtgtc tatttattac ggcgggcgag
     6781 gaagggaaag cgaaggagcg gtgggaaagg aatcccccgt agctgccggt gccgtgagag
     6841 gaggaggagg ccgcctgccg tgccggctca cgtctgccgc tccgccacgc aatttctgga
     6901 tgccgacagc ggagcaagtc caacggtgga gcggaactct cgagaggggt ccagaggcag
     6961 cgacagagat gccgtgccgt ctgcttcgct tggcccgacg cgacgctgct ggttcgctgg
     7021 ttggtgtccg ttagactcgt cgacggcgtt taacaggctg gcattatcta ctcgaaacaa
     7081 gaaaaatgtt tccttagttt ttttaatttc ttaaagggta tttgtttaat ttttagtcac
     7141 tttattttat tctattttat atctaaatta ttaaataaaa aaactaaaat agagttttag
     7201 ttttcttaat ttagaggcta aaatagaata aaatagatgt actaaaaaaa ttagtctata
     7261 aaaaccatta accctaaacc ctaaatggat gtactaataa aatggatgaa gtattatata
     7321 ggtgaagcta tttgcaaaaa aaaaggagaa cacatgcaca ctaaaaagat aaaactgtag
     7381 agtcctgttg tcaaaatact caattgtcct ttagaccatg tctaactgtt catttatatg
     7441 attctctaaa acactgatat tattgtagta ctatagatta tattattcgt agagtaaagt
     7501 ttaaatatat gtataaagat agataaactg cacttcaaac aagtgtgaca aaaaaaatat
     7561 gtggtaattt tttataactt agacatgcaa tgctcattat ctctagagag gggcacgacc
     7621 gggtcacgct gcactgcagg catgcaagct tcaccacttt gtaccatggt gctccactat
     7681 tgtcttctgc acgaagtggt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
     7741 cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt
     7801 atccgttcgt ccatttgtat gtgcatgcca accacagggt tccccagatc aggcgctggc
     7861 tgctgaaccc ccagccggaa ctgaccccac aaggccctag cgtttgcaat gcaccaggtc
     7921 atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc
     7981 gacctgctcg cgccacttct tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt
     8041 ttccagcttg agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc
     8101 cgtcggcgac agcttgcggt acttctccca tatgaatttc gtgtagtggt cgccagcaaa
     8161 cagcacgacg atttcctcgt cgatcaggac ctggcaacgg gacgttttct tgccacggtc
     8221 caggacgcgg aagcggtgca gcagcgacac cgattccagg tgcccaacgc ggtcggacgt
     8281 gaagcccatc gccgtcgcct gtaggcgcga caggcattcc tcggccttcg tgtaataccg
     8341 gccattgatc gaccagccca ggtcctggca aagctcgtag aacgtgaagg tgatcggctc
     8401 gccgataggg gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc
     8461 ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac
     8521 cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg
     8581 ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga
     8641 aagctgcatt tccttgatct gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc
     8701 gctgacctgt tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc
     8761 tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc tcctgttcaa gacgacgcga
     8821 acgctccacg gcggccgatg gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg
     8881 ctcgatcttg gccgtagctt gctggaccat cgagccgacg gactggaagg tttcgcgggg
     8941 cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc
     9001 gatcagttct tgcctgtatg ccttccggtc aaacgtccga ttcattcacc ctccttgcgg
     9061 gattgccccg actcacgccg gggcaatgtg cccttattcc tgatttgacc cgcctggtgc
     9121 cttggtgtcc agataatcca ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc
     9181 gtccttctcg tacttggtat tccgaatctt gccctgcacg aataccagcg accccttgcc
     9241 caaatacttg ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga agaagtcggt
     9301 gcgctcctgc ttgtcgccgg catcgttgcg ccacatctag gatctgccag gaaccgtaaa
     9361 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9421 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9481 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9541 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9601 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9661 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9721 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9781 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     9841 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9901 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9961 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    10021 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    10081 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    10141 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    10201 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    10261 agtgctgcaa tgataccgcg agaaccacgc tcaccggctc cagatttatc agcaataaac
    10321 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    10381 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    10441 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    10501 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    10561 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    10621 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    10681 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10741 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10801 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10861 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10921 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10981 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcaaggt
    11041 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    11101 ccgcgcacga attggccagc gctgccattt ttggggtgag gccgttcgcg gccgaggggc
    11161 gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg
    11221 gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg
    11281 tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg
    11341 cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg
    11401 tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg
    11461 tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac
    11521 atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag
    11581 ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag
    11641 tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg
    11701 aggtatccac aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc
    11761 gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag cccggtgagc
    11821 gtcgcaaagg agatcctgat ctgactgatg ggctgcctgt atcgagtggt gattttgtgc
    11881 cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca
    11941 aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac tggggtggat
    12001 gcagtgggcc ccac
//
