LOCUS       sequence-249652-       10303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Napin Promoter driven eGFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10303)
  AUTHORS   Wu G, Rossidivito G, Hu T, Berlyand Y, Poethig RS
  TITLE     Traffic lines: new tools for genetic analysis in Arabidopsis 
            thaliana.
  JOURNAL   Genetics. 2015 May;200(1):35-45. doi: 10.1534/genetics.114.173435. 
            Epub 2015 Feb 23.
  PUBMED    25711279
REFERENCE   2  (bases 1 to 10303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.114.173435"; journalName: "Genetics"; date: 
            "2015-05"; volume: "200"; issue: "1"; pages: "35-45"
COMMENT     Sequence Label: pNapin-eGFP
FEATURES             Location/Qualifiers
     source          1..10303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     213..235
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     227..244
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     228..244
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    248..304
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             join(1455..1457,1458..1460,1461..2174)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNDHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1500..1521)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1761..1780)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2108..2129
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      2175..2427
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    2434..2608
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2686..2710
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3135..3929
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5354..5942
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5843..5862
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6096..6113
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6128..6268
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6194..6213)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6354..6376
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6612..6806
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6872..7945)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8374..9003)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
      121 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      181 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      241 cagtgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta ccgagctcga
      301 attcgattcg gagctctctt catcggtgat tgattccttt aaagacttat gtttcttatc
      361 ttgcttctga ggcaagtatt cagttaccag ttaccactta tattctggac tttctgactg
      421 catcctcatt tttccaacat tttaaatttc actattggct gaatgcttct tctttgagga
      481 agaaacaatt cagatggcag aaatgtatca accaatgcat atatacaaat gtacctcttg
      541 ttctcaaaac atctatcgga tggttccatt tgctttgtca tccaattagt gactacttta
      601 tattattcac tcctctttat tactattttc atgcgaggtt gccatgtaca ttatatttgt
      661 aaggattgac gctattgagc gtttttcttc aattttcttt attttagaca tgggtatgaa
      721 atgtgtgtta gagttgggtt gaatgagata tacgttcaag tgaagtggca taccgttgtc
      781 gagtaaggat gacctaccca ttcttgagac aaatgttaca ttttagtatc agagtaaaat
      841 gtgtacctat aactcaaatt cgattgacat gtatccattc aacataaaat taaaccagcc
      901 tgcacctgca tccacatttc aagtattttc aaaccgttcg gctcctatcc accgggtgta
      961 acaagacgga ttccgaattt ggaagatttt gactcaaatt cccaatttat attgaccgtg
     1021 actaaatcaa ctttaacttc tataattctg attaagctcc caatttatat tcccaacggc
     1081 actacctcca aaatttatag actctcatcc ccttttaaac caacttagta aacgctcttt
     1141 tttttaattt tatgaagtta agtttttacc ttgtttttaa aaagaatcgt tcataagatg
     1201 ccatgccaga acattagcta cacgttacac atagcatgca gccgcggaga attgtttttc
     1261 ttcgccactt gtcactccct tcaaacacct aagagcttct ctctcacagc acacacatac
     1321 aatcacatgc gtgcatgcat tattacacgt gatcgccatg caaatctcct ttatagtcta
     1381 taaattaact catccgcttc actctttact caaaccaaaa ctcatcaata caaacaagat
     1441 taaaaacata ccccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     1501 tggtcgagct ggacggcgac gtaaacgacc acaagttcag cgtgtccggc gagggcgagg
     1561 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1621 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     1681 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1741 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1801 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1861 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     1921 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     1981 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     2041 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     2101 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     2161 agctgtacaa gtaagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     2221 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     2281 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     2341 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     2401 gcgcggtgtc atctatgtta ctagatcctc gagtttctcc ataataatgt gtgagtagtt
     2461 cccagataag ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa
     2521 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa
     2581 accaaaatcc agtactaaaa tccagatccc ccgaattaat tcggcgttaa ttcagtacat
     2641 taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata
     2701 tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact
     2761 cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca
     2821 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
     2881 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
     2941 tgtgatcacc gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa
     3001 aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa
     3061 ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag
     3121 gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa
     3181 aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg
     3241 gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg
     3301 atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg
     3361 tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg
     3421 tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg
     3481 cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct
     3541 tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt
     3601 gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa
     3661 agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca
     3721 tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg
     3781 acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac
     3841 agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat
     3901 attatatttt actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac
     3961 gtgagttttc gttccactga gcgctgagag atcccctcat aatttcccca aagcgtaacc
     4021 atgtgtgaat aaattttgag ctagtagggt tgcagccacg agtaagtctt cccttgttat
     4081 tgtgtagcca gaatgccgca aaacttccat gcctaagcga actgttgaga gtacgtttcg
     4141 atttctgact gtgttagcct ggaagtgctt gtcccaacct tgtttctgag catgaacgcc
     4201 cgcaagccaa catgttagtt gaagcatcag ggcgattagc agcatgatat caaaacgctc
     4261 tgagctgctc gttcggctat ggcgtaggcc tagtccgtag gcaggacttt tcaagtctcg
     4321 gaaggtttct tcaatctgca ttcgcttcga atagatatta acaagttgtt tgggtgttcg
     4381 aatttcaaca ggtaagttag ttgctagaac ccatggctcc tttgtcgacg ctgagtagat
     4441 tttaggtgac gggtggtgac aatgagtccg tgtcgagcgc tgattttttc ggcctttaga
     4501 gcgagattta tacaatagaa tttggcatga gattggattg cttttagtca gcctcttata
     4561 gcctaaagtc tttgagtgac tagatgacat atcatgtaag ttgctgatag gtttccagtt
     4621 ttccgctcct aggtctgcat attgtacttt tcctcttact cgacttaacc agtaccaacc
     4681 cagcttctca acggatttat accatggcac tttaaagcca gcatcactga caatgagcgg
     4741 tgtggtgtta ctcggtagaa tgctcgcaag gtcggctaga aattggtcat gagctttctt
     4801 tgaacattgc tctgaaagcg ggaacgcttt ctcataaaga gtaacagaac gaccgtgtag
     4861 tgcgactgaa gctcgcaata ccataagtcg tttttgctca cgaatatcag accagtcaac
     4921 aagtacaatg ggcatcgtat tgcccgaaca gataaagcta gcatgccaac ggtatacagc
     4981 gagtcgctct ttgtggaggt gacgattacc taacaatcgg tcgattcgtt tgatgttatg
     5041 ttttgttctc gctttggttg gcaggttacg gccaagttcg gtaagagtga gagttttaca
     5101 gtcaagtaat gcgtggcaag ccaacgttaa gctgttgagt cgttttaagt gtaattcggg
     5161 gcagaattgg taaagagagt cgtgtaaaat atcgagttcg cacatcttgt tgtctgatta
     5221 ttgatttttc gcgaaaccat ttgatcatat gacaagatgt gtatccacct taacttaatg
     5281 atttttacca aaatcattag gggattcatc agcactgagc gtcagacccc gtagaaaaga
     5341 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5401 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5461 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     5521 taggccacca cttcaagaac tctgtagcac ggcctacata cctcgctctg ctaatcctgt
     5581 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5641 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5701 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5761 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5821 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5881 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5941 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6001 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6061 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     6121 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     6181 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     6241 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     6301 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     6361 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat
     6421 gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg
     6481 gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg
     6541 ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct
     6601 ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt
     6661 tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt
     6721 tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg
     6781 ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta
     6841 gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga
     6901 tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat
     6961 cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga
     7021 actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct
     7081 tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa
     7141 agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca
     7201 tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct
     7261 tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct
     7321 tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt
     7381 cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg
     7441 gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg
     7501 ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg
     7561 gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc
     7621 gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg
     7681 tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct
     7741 tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag
     7801 cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg
     7861 cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg
     7921 ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt
     7981 gcagggccgg caggcaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat
     8041 ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc
     8101 cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt
     8161 attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg
     8221 tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc
     8281 tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg
     8341 tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc
     8401 ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt
     8461 gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg
     8521 cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc
     8581 ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac
     8641 cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag
     8701 cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc
     8761 gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc
     8821 gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc
     8881 ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag
     8941 atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt
     9001 catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca
     9061 tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct
     9121 tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt
     9181 tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc
     9241 atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt
     9301 ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca
     9361 atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc
     9421 gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca
     9481 cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg
     9541 tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc
     9601 ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc
     9661 ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg
     9721 cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta
     9781 gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg
     9841 ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt
     9901 gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca
     9961 gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat
    10021 tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag
    10081 cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac
    10141 gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag
    10201 gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc
    10261 ttttaaatat ccgattattc taataaacgc tcttttctct tag
//
