LOCUS       sequence-249661-       11015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Digestion by BstXI and BamHI to release the backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11015)
  AUTHORS   Wang C, Lu T, Emanuel G, Babcock HP, Zhuang X
  TITLE     Imaging-based pooled CRISPR screening reveals regulators of lncRNA 
            localization.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 May 28;116(22):10842-10851. doi: 
            10.1073/pnas.1903808116. Epub 2019 May 13.
  PUBMED    31085639
REFERENCE   2  (bases 1 to 11015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1903808116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-05-28- 28"; volume: "116"; issue: "22"; pages: 
            "10842-10851"
COMMENT     Sequence Label: library backbone 3'LTR gRNA and barcode
FEATURES             Location/Qualifiers
     source          1..11015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..2813
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2814..3752
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3709..3729
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3773..4369
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3773..3792)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4269..4289
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4379..4432
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4448..5152
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(4590..4608)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             5168..5188
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5213..5801
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5266..5286)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5684..5695)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5958..5978
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6129..6148
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     LTR             6820..7000
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7026..7043)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7032..7256
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7302..7730
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7389..7408)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7599..7620
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7739..7759)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7744..8073
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7924..8059
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7986..8005
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8121..8168
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8187..8561
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8691..8812
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8728..8747)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8782..8801
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8861..8877)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8861..8877)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8874..8896)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8885..8901
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8909..8915,8916..8933,8934..8939))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8954..8975
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9092..9109)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9263..9851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9343..9362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10022..10813,10814..10882))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10645..10664
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10883..10987)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2641 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2701 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2761 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2821 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2881 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2941 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     3001 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     3061 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     3121 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     3181 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     3241 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     3301 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3361 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3421 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3481 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3541 attagttctc gcgcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3601 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3661 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3721 cagtggttca aagttttttt cttccatttc aggtgtcgtg agctagccca ccatgaccga
     3781 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct
     3841 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
     3901 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     3961 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     4021 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     4081 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     4141 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     4201 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     4261 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     4321 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgccg gatcgggaga
     4381 gggcagagga agtctgctaa catgcggtga cgtcgaggag aatcctggcc caccggtcgc
     4441 caccagcgtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     4501 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     4561 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     4621 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     4681 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     4741 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     4801 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     4861 cgtaatgcag aagaaaacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     4921 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     4981 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     5041 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     5101 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agggtggtgg
     5161 tactagtccc aagaagaagc gcaaggtgta agacccggaa tcgatagatc ctaatcaacc
     5221 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     5281 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     5341 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     5401 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     5461 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     5521 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     5581 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     5641 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     5701 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttggccttcg
     5761 ccctcagacg agtcggatct ccctttgggc cgcctccccg cagtcgactt taagaccaat
     5821 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     5881 gctaattcac tcccaacgaa cacaagattt caccattatc gtttcagacc cacctcccaa
     5941 ccccgagggg acccagagag ggcctatttc ccatgattcc ttcatatttg catatacgat
     6001 acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag atattagtac
     6061 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
     6121 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
     6181 atatcttgtg gaaagccaga aacatggttt cccacctgtc tccacgcgtt tcagagctaa
     6241 gcacaagagt gcatagcaag ttgaaataag gctagtccgt ttacaacttg aaaaagtggc
     6301 acccgagtcg ggtgcttttt tttcgggtac cagatccgat tggaaccgtc ccaagcttgc
     6361 gaaagttcaa cgggaccgtg gccgatgttt cgattagtcg tcgcaacgcc aggcaactca
     6421 tgcagcagat tccgctacgc tccgattcga tcaaatcgcg cacgagcgct aatcgccgga
     6481 cctaaaggtc gatgccctaa tccacgtgct tcccgcaaat cgatccacta ccgtcgatcc
     6541 cgtgtccata gcaaagccgg tacgacaacc gtttcccgtc cgagaaaagg ccgccggtca
     6601 tatggatact gaatccgtcg ttccagacgc gagtacccac agttcaactc gtgcgcactt
     6661 gcgagcaaaa agcccgtcta aacgcggcga ggtacattgg gccgtccgtc atctcgtttt
     6721 cgatgtctag atcagtcaac caggattttt caggatccat tacgataaat accgtcgaca
     6781 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     6841 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     6901 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     6961 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg
     7021 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     7081 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     7141 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     7201 gggggaggat tgggaacaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     7261 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     7321 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     7381 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     7441 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     7501 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     7561 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     7621 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     7681 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     7741 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7801 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     7861 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     7921 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7981 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     8041 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     8101 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
     8161 aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca
     8221 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
     8281 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
     8341 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
     8401 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
     8461 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
     8521 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg
     8581 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     8641 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     8701 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8761 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     8821 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     8881 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     8941 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     9001 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     9061 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     9121 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     9181 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     9241 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     9301 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9361 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9421 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     9481 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     9541 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9601 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9661 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     9721 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9781 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9841 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9901 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9961 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    10021 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    10081 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    10141 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    10201 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    10261 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    10321 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    10381 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    10441 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    10501 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    10561 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    10621 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    10681 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    10741 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10801 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10861 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10921 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10981 ttccgcgcac atttccccga aaagtgccac ctgac
//
