LOCUS       sequence-249670-        8574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8574)
  AUTHORS   Yi SJ, Lee HT, Groffen J, Heisterkamp N
  TITLE     Bcr/Abl P190 interaction with Spa-1, a GTPase activating protein for
            the small GTPase Rap1.
  JOURNAL   Int J Mol Med. 2008 Oct . 22(4):453-8.
  PUBMED    18813851
REFERENCE   2  (bases 1 to 8574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Med. 2008 Oct . 22(4):453-8."
COMMENT     Sequence Label: pSG5-ABL
FEATURES             Location/Qualifiers
     source          1..8574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        23..219
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          250..822
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     830..849
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     877..896
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        877..895
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5436..5557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5473..5492)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5527..5546
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5591..5608)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5762..6350)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5842..5861)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6521..7312,7313..7381))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7144..7163
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7382..7486)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7554..7572
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7610..7632)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7732..7751
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7818..8273)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7955..7976)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8167..8186
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8399..8421
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8413..8430
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8414..8430
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8467..8487)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      181 tagtgaagag gcttttttgg aggcctaggc ttttgcaaaa agctccggat cgatcctgag
      241 aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat tgtaaaattc
      301 atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag atgtcccttg
      361 tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg ttgacaacca
      421 ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt tagcttgcat
      481 ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta ctttctctaa
      541 tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca gttttagaga
      601 acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc tggctggcgt
      661 ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc tttctcttta
      721 tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc
      781 ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
      841 gttattgtgc tgtctcatca ttttggcaaa gaattgtaat acgactcact atagggcgaa
      901 ttccttttaa aaagcggact taaaagttgc tggcaatgca ttccttttcg tcagagtcga
      961 gggcaaactc gctgaaatct gggtgacccg tgtccttttc cggagagcaa agcagagaag
     1021 cgagagcggc cactagttcg gcaggaaatt tgttggaaga tgaagaagct aagatagggg
     1081 gttggtgact tccacaggaa aagttctgga ggagtagcca aagaccatca gcgtttcctt
     1141 tatgtgtgag aattgaaatg actagcatta ttgacccttt tcagcatccc ctgtgaatat
     1201 ttctgtttag gtttttcttc ttgaaaagaa attgttattc agcccgttta aaacaaatca
     1261 agaaactttt gggtaacatt gcaattacat gaaattgata accgcgaaaa taattggaac
     1321 tcctgcttgc aagtgtcaac ctaaaaaaag tgcttccttt tgttatggaa gatgtctttc
     1381 tgtgattgac ttcaattgct gacttgtgga gatgcagcga atgtgaaatc ccacgtatat
     1441 gccatttccc tctacgctcg ctgaccgttc tggaagatct tgaaccctct tctggaaagg
     1501 ggtacctatt attactttat ggggcagcag cctggaaaag tacttgggga ccaaagaagg
     1561 ccaagcttgc ctgccctgca ttttatcaaa ggagcaggga agaaggaatc atcgaggcat
     1621 gggggtccac actgcaatgt ttttgtggaa catgaagccc ttcagcggcc agtagcatct
     1681 gactttgagc ctcagggtct gagtgaagcc gctcgttgga actccaagga aaaccttctc
     1741 gctggaccca gtgaaaatga ccccaacctt ttcgttgcac tgtatgattt tgtggccagt
     1801 ggagataaca ctctaagcat aactaaaggt gaaaagctcc gggtcttagg ctataatcac
     1861 aatggggaat ggtgtgaagc ccaaaccaaa aatggccaag gctgggtccc aagcaactac
     1921 atcacgccag tcaacagtct ggagaaacac tcctggtacc atgggcctgt gtcccgcaat
     1981 gccgctgagt atctgctgag cagcgggatc aatggcagct tcttggtgcg tgagagtgag
     2041 agcagtcctg gccagaggtc catctcgctg agatacgaag ggagggtgta ccattacagg
     2101 atcaacactg cttctgatgg caagctctac gtctcctccg agagccgctt caacaccctg
     2161 gccgagttgg ttcatcatca ttcaacggtg gccgacgggc tcatcaccac gctccattat
     2221 ccagccccaa agcgcaacaa gcccactgtc tatggtgtgt cccccaacta cgacaagtgg
     2281 gagatggaac gcacggacat caccatgaag cacaagctgg gcgggggcca gtacggggag
     2341 gtgtacgagg gcgtgtggaa gaaatacagc ctgacggtgg ccgtgaagac cttgaaggag
     2401 gacaccatgg aggtggaaga gttcttgaaa gaagctgcag tcatgaaaga gatcaaacac
     2461 cctaacctgg tgcagctcct tggggtctgc acccgggagc ccccgttcta tatcatcact
     2521 gagttcatga cctacgggaa cctcctggac tacctgaggg agtgcaaccg gcaggaggtg
     2581 aacgccgtgg tgctgctgta catggccact cagatctcgt cagccatgga gtacctggag
     2641 aagaaaaact tcatccacag agatcttgct gcccgaaact gcctggtagg ggagaaccac
     2701 ttggtgaagg tagctgattt tggcctgagc aggttgatga caggggacac ctacacagcc
     2761 catgctggag ccaagttccc catcaaatgg actgcacccg agagcctggc ctacaacaag
     2821 ttctccatca agtccgacgt ctgggcattt ggagtattgc tttgggaaat tgctacctat
     2881 ggcatgtccc cttacccggg aattgacctg tcccaggtgt atgagctgct agagaaggac
     2941 taccgcatgg agcgcccaga aggctgccca gagaaggtct atgaactcat gcgagcatgt
     3001 tggcagtgga atccctctga ccggccctcc tttgctgaaa tccaccaagc ctttgaaaca
     3061 atgttccagg aatccagtat ctcagacgaa gtggaaaagg agctggggaa acaaggcgtc
     3121 cgtggggctg tgagtacctt gctgcaggcc ccagagctgc ccaccaagac gaggacctcc
     3181 aggagagctg cagagcacag agacaccact gacgtgcctg agatgcctca ctccaagggc
     3241 cagggagaga gcgatcctct ggaccatgag cctgccgtgt ctccattgct ccctcgaaaa
     3301 gagcgaggtc ccccggaggg cggcctgaat gaagatgagc gccttctccc caaagacaaa
     3361 aagaccaact tgttcagcgc cttgatcaag aagaagaaga agacagcccc aacccctccc
     3421 aaacgcagca gctccttccg ggagatggac ggccagccgg agcgcagagg ggccggcgag
     3481 gaagagggcc gagacatcag caacggggca ctggctttca cccccttgga cacagctgac
     3541 ccagccaagt ccccaaagcc cagcaatggg gctggggtcc ccaatggagc cctccgggag
     3601 tccgggggct caggcttccg gtctccccac ctgtggaaga agtccagcac gctgaccagc
     3661 agccgcctag ccaccggcga ggaggagggc ggtggcagct ccagcaagcg cttcctgcgc
     3721 tcttgctccg cctcctgcgt tccccatggg gccaaggaca cggagtggag gtcagtcacg
     3781 ctgcctcggg acttgcagtc cacgggaaga cagtttgact cgtccacatt tggagggcac
     3841 aaaagtgaga agccggctct gcctcggaag agggcagggg agaacaggtc tgaccaggtg
     3901 acccgaggca cagtaacgcc tccccccagg ctggtgaaaa agaatgagga agctgctgat
     3961 gaggtcttca aagacatcat ggagtccagc ccgggctcca gcctgcccaa cctgactcca
     4021 aaacccctcc ggcggcaggt caccgtggcc cctgcctcgg gcctccccca caaggaagaa
     4081 gctggaaagg gcagtgcctt agggacccct gctgcagctg agccagtgac ccccaccagc
     4141 aaagcaggct caggtgcacc agggggcacc agcaagggcc ccgccgagga gtccagagtg
     4201 aggaggcaca agcactcctc tgagtcgcca gggagggaca aggggaaatt gtccaggctc
     4261 aaacctgccc cgccgccccc accagcagcc tctgcaggga aggctggagg aaagccctcg
     4321 cagagcccga gccaggaggc ggccggggag gcagtcctgg gcgcaaagac aaaagccacg
     4381 agtctggttg atgctgtgaa cagtgacgct gccaagccca gccagccggg agagggcctc
     4441 aaaaagcccg tgctcccggc cactccaaag ccacagtccg ccaagccgtc ggggaccccc
     4501 atcagcccag cccccgttcc ctccacgttg ccatcagcat cctcggccct ggcaggggac
     4561 cagccgtctt ccaccgcctt catccctctc atatcaaccc gagtgtctct tcggaaaacc
     4621 cgccagcctc cagagcggat cgccagcggc gccatcacca agggcgtggt cctggacagc
     4681 accgaggcgc tgtgcctcgc catctctagg aactccgagc agatggccag ccacagcgca
     4741 gtgctggagg ccggcaaaaa cctctacacg ttctgcgtga gctatgtgga ttccatccag
     4801 caaatgagga acaagtttgc cttccgagag gccatcaaca aactggagaa taatctccgg
     4861 gagcttcaga tctgcccggc gacagcaggc agtggtccag cggccactca ggacttcagc
     4921 aagctcctca gttcggtgaa ggaaatcagt gacatagtgc agaggtagca gcagtcaggg
     4981 gtcaggtgtc aggcccgtcg gagctgcctg cagcacatgc gggctcgccc atacccgtga
     5041 cagtggctga caagggacta gtgagtcagc accttggccc aggagctctg cgccaggcag
     5101 agctgagggc cctgtggagt ccagctctac tacctacgtt tgcaccgcct gccctcccgc
     5161 accttcctcc tccccgctcc gtctctgtcc tcgaatttta tctgtggagt tcctgctccg
     5221 tggactgcag tcggcatgcc aggacccgcc agccccgctc ccacctagtg ccccagactg
     5281 agctctccag gccaggtggg aacggctgat gtggactgtc tttttcattt ttttctctct
     5341 ggagcccctc ctcccccggc tgggcctcct tcttccactt ctccaagaat ggaagcctga
     5401 actgaggaat tcggatccag atcttattaa agcagaactt gtttattgca gcttataatg
     5461 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5521 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggtcg actctagact
     5581 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5641 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5701 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5761 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5821 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5881 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5941 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6001 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6061 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6121 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6181 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6241 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6301 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6361 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6421 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6481 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6541 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     6601 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     6661 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6721 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6781 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     6841 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6901 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     6961 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7021 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7081 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7141 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7201 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7261 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7321 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7381 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7441 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7501 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     7561 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     7621 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     7681 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     7741 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     7801 gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     7861 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     7921 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     7981 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     8041 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     8101 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     8161 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     8221 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg
     8281 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     8341 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     8401 cagtcacgac gttgtaaaac gacggccagt gaattgtcga cttctgaggc ggaaagaacc
     8461 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     8521 gtatgcaaag catgcatctc aattagtcag caaggaaagt ccccaggctc ccca
//
