LOCUS       sequence-249672-        6228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For in vitro translation of human ELL2 with 30 nt pA tail.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Martincic K, Alkan SA, Cheatle A, Borghesi L, Milcarek C
  TITLE     Transcription elongation factor ELL2 directs immunoglobulin 
            secretion in plasma cells by stimulating altered RNA processing.
  JOURNAL   Nat Immunol. 2009 Oct;10(10):1102-9. doi: 10.1038/ni.1786. Epub 2009
            Sep 13.
  PUBMED    19749764
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.1786"; 
            journalName: "Nat Immunol"; date: "2009-10"; volume: "10"; issue: 
            "10"; pages: "1102-9"
COMMENT     Sequence Label: ELL2mRNA+polyA
FEATURES             Location/Qualifiers
     source          1..6228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(556..567)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(3333..3351)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3372..3388)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3372..3388)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3385..3407)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3396..3412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3420..3426,3427..3444,3445..3450))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3465..3486
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3603..3620)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3774..4362)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3854..3873)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4533..5324,5325..5393))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5156..5175
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5394..5498)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5524..5979)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5661..5682)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5873..5892
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6106..6128
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6120..6137
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6121..6137
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6144..6163
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6144..6162
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(6195..6228)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggatcaggcc aaatcggccg agctcgaatt cgtcgagagc gggataagtt ggcgcgtcac
       61 ctagacgtca gcctcttacc tctcggacgt cccgtcccag gagcaaaact ctagtgactt
      121 tgggcccgcc cccgtgagag tggagggcgg ccgatgaggg cggttctgaa gggaacaaga
      181 ccaatgagac gagcccgggg cggggcgcgg ggccggaggg tggggcgggt ggcggcggtg
      241 gcagtccgag gtctggctcc gcgcagtata tgacagtacg tcagcagcgg gatggcccta
      301 gcagtggcgg cggctgcaga agcccaagca gccgcggccg cagtggaggc tagagccgga
      361 gcggcggcgg cggcggcacc ccggggagtt taagatggcg gcggggggga cagggggcct
      421 gcgggaggag cagcgctatg ggctgtcgtg cggacggctg gggcaggaca acatcaccgt
      481 actgcatgtg aagctcaccg agacggcgat ccgggcgctc gagacttacc agagccacaa
      541 gaatttaatt ccttttcgac cttcaatcca gttccaagga ctccacgggc ttgtcaaaat
      601 tcccaaaaat gatcccctca atgaagttca taactttaac ttttatttgt caaatgtggg
      661 caaagacaac cctcagggca gctttgactg catccagcaa acattctcca gctctggagc
      721 ctcccagctc aattgcctgg gatttataca agataaaatt acagtgtgtg caacaaacga
      781 ctcgtatcag atgacacgag aaagaatgac ccaggcagag gaggaatccc gcaaccgaag
      841 cacaaaagtt atcaaacccg gtggaccata tgtagggaaa agagtgcaaa ttcggaaagc
      901 acctcaagct gtttcagata cagttcctga gaggaaaagg tcaaccccca tgaaccctgc
      961 aaatacaatt cgaaagacac atagcagcag caccatctct cagaggccat acagggacag
     1021 ggtgattcac ttactggccc tgaaggccta caagaaaccg gagctacttg ctagactcca
     1081 gaaagatggt gtcaatcaaa aagacaagaa ctccctggga gcaattctgc aacaggtagc
     1141 caatctgaat tctaaggacc tctcatatac cttaaaggat tatgttttta aagagcttca
     1201 aagagactgg cctggataca gtgaaataga cagacggtca ttggagtcag tgctctctag
     1261 aaaactaaat ccgtctcaga atgctacagg caccagccgt tcagaatctc ctgtatgttc
     1321 tagtagagat gctgtatctt ctcctcagaa acggcttttg gattcagagt ttattgatcc
     1381 tttaatgaat aaaaaagccc gaatatctca cctgacgaac agagtaccac caacactaaa
     1441 tggtcatttg aatcccacca gtgaaaaatc ggctgcaggc ctcccactgc cccctgcggc
     1501 tgctgccatc cccacccctc caccgctgcc ttcaacctat ctgcccatct cacatcctcc
     1561 tcagattgta aattctaact ccaactcccc tagcactcca gaaggccggg ggactcaaga
     1621 cctacctgtt gacagtttta gtcaaaacga tagtatctat gaggaccagc aagacaaata
     1681 tacctctagg acttctctgg aaaccttacc ccctggttcc gttctactaa agtgtccaaa
     1741 gcctatggaa gaaaaccatt caatgtctca caaaaagtcc aaaaagaagt ctaaaaaaca
     1801 taaggaaaag gaccaaataa aaaagcacga cattgagact attgaggaaa aggaggaaga
     1861 tcttaagaga gaagaggaaa ttgccaagct aaataactcc agtccaaatt ccagtggagg
     1921 agttaaagag gattgcactg cctccatgga accttcagca attgaactcc cagattattt
     1981 gataaaatat atcgctatcg tctcctatga gcaacgccag aattataagg atgacttcaa
     2041 tgcagagtat gatgagtaca gagctttgca tgccaggatg gagactgtag ctagaagatt
     2101 tatcaaacta gatgcacaaa gaaagcgcct ttctccaggc tcaaaagagt atcagaatgt
     2161 tcatgaagaa gtcttacaag aatatcagaa gataaagcag tctagtccca attaccatga
     2221 agaaaaatac agatgtgaat atcttcataa caagctggct cacatcaaaa ggctaatagg
     2281 tgaatttgac caacagcaag cagagtcatg gtcctagaac tctgcttgga ccagaagatg
     2341 tgaataaact taagcttgga aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa acgcgtaacc
     2401 ccccattgca atggttgcct taaggtgttt gctagttgtg tacatagtgt ggttaatcat
     2461 tagctacact gcttcccact tgattagagc aatgggaagc atactgtggc ctaccagcat
     2521 ctggaagtgt gtgctcgatc tgtatgtgtg cagaggtggt gtggatgtga gcgtgcatga
     2581 aggaaaaaaa gctgctactc ctagtaggcc aaacgctcag gttaaacaac tgacgagtgt
     2641 tactgtaggg tgtttttttg tttttttgtt ttttttcttc tatcaaattg ctacttttgt
     2701 tgtggaagac aaaagcattt ccatttcaac gagtttgtca gctttattaa tgttgggcaa
     2761 aaattgatat gtcatgaaaa tgaaacagat ctatagtttt gggacaaaat tataaaatga
     2821 aatgtgtagg taacctattt atatactgct ataaagtatt ttttgaagag agatatgcaa
     2881 agaagctatt acctacataa gaggtatatt taaagatttt ttttttttca tcctggtgcc
     2941 aggaatataa aaaagagtgg atatatttaa ccataacata ctgtgattca tcaaacagca
     3001 caaactttca tttcatggag tttatctgtt gacattgatt taaactgtca cttgttttat
     3061 catgtgggaa cataagttat gtggtcaaaa atataaggat tttgaattaa tgttgattca
     3121 agttgtattg tcttttcaaa gtgctgccag ttgaaaaggg aagcattatg tttacaaatc
     3181 tgttttgaaa tgtttgccaa aattttggta gtgtctttaa taaagatgtt tgtctccagc
     3241 atccagnaaa aaaaaaaaaa aaactctcca gcgctggatc cggccataag ggcctgatcc
     3301 ttcgaggggg ggcccggtac cagcttttgt tccctttagt gagggttaat ttcgagcttg
     3361 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     3421 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     3481 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     3541 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     3601 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     3661 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     3721 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     3781 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     3841 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3901 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3961 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4021 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4081 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4141 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4201 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     4261 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     4321 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4381 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4441 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4501 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     4561 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     4621 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     4681 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     4741 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     4801 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     4861 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     4921 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     4981 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5041 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5101 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     5161 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     5221 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     5281 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     5341 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     5401 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     5461 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     5521 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     5581 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     5641 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     5701 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     5761 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     5821 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     5881 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     5941 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc
     6001 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     6061 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     6121 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg gagctccacc
     6181 gcggtggcgg ccgcataact tcgtatagca tacattatac gaagttat
//
