LOCUS       sequence-249675-        7748 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses full-length human ELL2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7748)
  AUTHORS   Martincic K, Alkan SA, Cheatle A, Borghesi L, Milcarek C
  TITLE     Transcription elongation factor ELL2 directs immunoglobulin 
            secretion in plasma cells by stimulating altered RNA processing.
  JOURNAL   Nat Immunol. 2009 Oct;10(10):1102-9. doi: 10.1038/ni.1786. Epub 2009
            Sep 13.
  PUBMED    19749764
REFERENCE   2  (bases 1 to 7748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.1786"; 
            journalName: "Nat Immunol"; date: "2009-10"; volume: "10"; issue: 
            "10"; pages: "1102-9"
COMMENT     Sequence Label: Full human ELL2
FEATURES             Location/Qualifiers
     source          1..7748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1196..1215
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1256..1273
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1277..1309
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(1313..1321,1322..1336)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(1546..1557)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3406..3423)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3412..3636
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3682..4110
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3769..3788)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3979..4000
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4119..4139)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4124..4453
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4304..4439
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4366..4385
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4501..4548
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4567..4941
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5071..5192
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5108..5127)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5162..5181
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5241..5257)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5241..5257)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5254..5276)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5265..5281
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5289..5295,5296..5313,5314..5319))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5334..5355
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5472..5489)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5643..6231)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5723..5742)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6402..7193,7194..7262))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7025..7044
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7263..7367)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7442..7461)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt accatggggg gttctcatca
     1261 tcatcatcat catggtatgg ctagcatgac tggtggacag caaatgggtc gggatctgta
     1321 cgacgatgac gataaggtac ctaaggatcc agtgtggtgg aattctgcag atggggagtt
     1381 taagatggcg gcggggggga cagggggcct gcgggaggag cagcgctatg ggctgtcgtg
     1441 cggacggctg gggcaggaca acatcaccgt actgcatgtg aagctcaccg agacggcgat
     1501 ccgggcgctc gagacttacc agagccacaa gaatttaatt ccttttcgac cttcaatcca
     1561 gttccaagga ctccacgggc ttgtcaaaat tcccaaaaat gatcccctca atgaagttca
     1621 taactttaac ttttatttgt caaatgtggg caaagacaac cctcagggca gctttgactg
     1681 catccagcaa acattctcca gctctggagc ctcccagctc aattgcctgg gatttataca
     1741 agataaaatt acagtgtgtg caacaaacga ctcgtatcag atgacacgag aaagaatgac
     1801 ccaggcagag gaggaatccc gcaaccgaag cacaaaagtt atcaaacccg gtggaccata
     1861 tgtagggaaa agagtgcaaa ttcggaaagc acctcaagct gtttcagata cagttcctga
     1921 gaggaaaagg tcaaccccca tgaaccctgc aaatacaatt cgaaagacac atagcagcag
     1981 caccatctct cagaggccat acagggacag ggtgattcac ttactggccc tgaaggccta
     2041 caagaaaccg gagctacttg ctagactcca gaaagatggt gtcaatcaaa aagacaagaa
     2101 ctccctggga gcaattctgc aacaggtagc caatctgaat tctaaggacc tctcatatac
     2161 cttaaaggat tatgttttta aagagcttca aagagactgg cctggataca gtgaaataga
     2221 cagacggtca ttggagtcag tgctctctag aaaactaaat ccgtctcaga atgctacagg
     2281 caccagccgt tcagaatctc ctgtatgttc tagtagagat gctgtatctt ctcctcagaa
     2341 acggcttttg gattcagagt ttattgatcc tttaatgaat aaaaaagccc gaatatctca
     2401 cctgacgaac agagtaccac caacactaaa tggtcatttg aatcccacca gtgaaaaatc
     2461 ggctgcaggc ctcccactgc cccctgcggc tgctgccatc cccacccctc caccgctgcc
     2521 ttcaacctat ctgcccatct cacatcctcc tcagattgta aattctaact ccaactcccc
     2581 tagcactcca gaaggccggg ggactcaaga cctacctgtt gacagtttta gtcaaaacga
     2641 tagtatctat gaggaccagc aagacaaata tacctctagg acttctctgg aaaccttacc
     2701 ccctggttcc gttctactaa agtgtccaaa gcctatggaa gaaaaccatt caatgtctca
     2761 caaaaagtcc aaaaagaagt ctaaaaaaca taaggaaaag gaccaaataa aaaagcacga
     2821 cattgagact attgaggaaa aggaggaaga tcttaagaga gaagaggaaa ttgccaagct
     2881 aaataactcc agtccaaatt ccagtggagg agttaaagag gattgcactg cctccatgga
     2941 accttcagca attgaactcc cagattattt gataaaatat atcgctatcg tctcctatga
     3001 gcaacgccag aattataagg atgacttcaa tgcagagtat gatgagtaca gagctttgca
     3061 tgccaggatg gagactgtag ctagaagatt tatcaaacta gatgcacaaa gaaagcgcct
     3121 ttctccaggc tcaaaagagt atcagaatgt tcatgaagaa gtcttacaag aatatcagaa
     3181 gataaagcag tctagtccca attaccatga agaaaaatac agatgtgaat atcttcataa
     3241 caagctggct cacatcaaaa ggctaatagg tgaatttgac caacagcaag cagagtcatg
     3301 gtcctagaac tctgcttgga ccagaagatg tgaataaact taagcttatt tatttaaaat
     3361 tccaaatgag ttgctctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     3421 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     3481 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     3541 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     3601 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     3661 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     3721 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     3781 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     3841 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     3901 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     3961 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4021 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4081 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     4141 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4201 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4261 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     4321 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4381 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4441 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
     4501 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     4561 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
     4621 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
     4681 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
     4741 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
     4801 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
     4861 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
     4921 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat
     4981 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     5041 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     5101 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     5161 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     5221 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     5281 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     5341 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     5401 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     5461 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5521 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5581 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5641 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5701 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5761 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     5821 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     5881 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     5941 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6001 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6061 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     6121 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6181 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6241 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6301 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6361 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6421 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6481 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6541 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6601 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6661 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     6721 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     6781 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     6841 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     6901 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     6961 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7021 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7081 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7141 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7201 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7261 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7321 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7381 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
     7441 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     7501 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     7561 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     7621 gatatacgcg ttgacattga ttattgacta ggcttttgca aaaagctttg caaagatgga
     7681 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag
     7741 tgggaatt
//
