LOCUS       sequence-249679-        6231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For in vitro translation of human a frameshift ELL2 that results in 
            a 58 kDa product from internal initiation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Martincic K, Alkan SA, Cheatle A, Borghesi L, Milcarek C
  TITLE     Transcription elongation factor ELL2 directs immunoglobulin 
            secretion in plasma cells by stimulating altered RNA processing.
  JOURNAL   Nat Immunol. 2009 Oct;10(10):1102-9. doi: 10.1038/ni.1786. Epub 2009
            Sep 13.
  PUBMED    19749764
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ni.1786"; 
            journalName: "Nat Immunol"; date: "2009-10"; volume: "10"; issue: 
            "10"; pages: "1102-9"
COMMENT     Sequence Label: frameshift ELL2
FEATURES             Location/Qualifiers
     source          1..6231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(594..605)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(3370..3388)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3409..3425)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3409..3425)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3422..3444)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3433..3449
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3457..3463,3464..3481,3482..3487))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3502..3523
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3640..3657)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3811..4399)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3891..3910)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4570..5361,5362..5430))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5193..5212
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5431..5535)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5561..6016)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5698..5719)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5910..5929
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6143..6165
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6157..6174
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6158..6174
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6181..6200
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6181..6199
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatggatca ggccaaatcg gccgagctcg
       61 aattcgtcga gagcgggata agttggcgcg tcacctagac gtcagcctct tacctctcgg
      121 acgtcccgtc ccaggagcaa aactctagtg actttgggcc cgcccccgtg agagtggagg
      181 gcggccgatg agggcggttc tgaagggaac aagaccaatg agacgagccc ggggcggggc
      241 gcggggccgg agggtggggc gggtggcggc ggtggcagtc cgaggtctgg ctccgcgcag
      301 tatatgacag tacgtcagca gcgggatggc cctagcagtg gcggcggctg cagaagccca
      361 agcagccgcg gccgcagtgg aggctagagc cggagcggcg gcggcggcgg caccccgggg
      421 agtttaagat ggcggcgggg gggacagggg gcctgcggga ggagcagcgc tatgggctgt
      481 cgtgcggacg gctggggcag gacaacatca ccgtactgca tgtgaagctc accgagacgg
      541 cgatccgggc gctcgatcga gacttaccag agccacaaga atttaattcc ttttcgacct
      601 tcaatccagt tccaaggact ccacgggctt gtcaaaattc ccaaaaatga tcccctcaat
      661 gaagttcata actttaactt ttatttgtca aatgtgggca aagacaaccc tcagggcagc
      721 tttgactgca tccagcaaac attctccagc tctggagcct cccagctcaa ttgcctggga
      781 tttatacaag ataaaattac agtgtgtgca acaaacgact cgtatcagat gacacgagaa
      841 agaatgaccc aggcagagga ggaatcccgc aaccgaagca caaaagttat caaacccggt
      901 ggaccatatg tagggaaaag agtgcaaatt cggaaagcac ctcaagctgt ttcagataca
      961 gttcctgaga ggaaaaggtc aacccccatg aaccctgcaa atacaattcg aaagacacat
     1021 agcagcagca ccatctctca gaggccatac agggacaggg tgattcactt actggccctg
     1081 aaggcctaca agaaaccgga gctacttgct agactccaga aagatggtgt caatcaaaaa
     1141 gacaagaact ccctgggagc aattctgcaa caggtagcca atctgaattc taaggacctc
     1201 tcatatacct taaaggatta tgtttttaaa gagcttcaaa gagactggcc tggatacagt
     1261 gaaatagaca gacggtcatt ggagtcagtg ctctctagaa aactaaatcc gtctcagaat
     1321 gctacaggca ccagccgttc agaatctcct gtatgttcta gtagagatgc tgtatcttct
     1381 cctcagaaac ggcttttgga ttcagagttt attgatcctt taatgaataa aaaagcccga
     1441 atatctcacc tgacgaacag agtaccacca acactaaatg gtcatttgaa tcccaccagt
     1501 gaaaaatcgg ctgcaggcct cccactgccc cctgcggctg ctgccatccc cacccctcca
     1561 ccgctgcctt caacctatct gcccatctca catcctcctc agattgtaaa ttctaactcc
     1621 aactccccta gcactccaga aggccggggg actcaagacc tacctgttga cagttttagt
     1681 caaaacgata gtatctatga ggaccagcaa gacaaatata cctctaggac ttctctggaa
     1741 accttacccc ctggttccgt tctactaaag tgtccaaagc ctatggaaga aaaccattca
     1801 atgtctcaca aaaagtccaa aaagaagtct aaaaaacata aggaaaagga ccaaataaaa
     1861 aagcacgaca ttgagactat tgaggaaaag gaggaagatc ttaagagaga agaggaaatt
     1921 gccaagctaa ataactccag tccaaattcc agtggaggag ttaaagagga ttgcactgcc
     1981 tccatggaac cttcagcaat tgaactccca gattatttga taaaatatat cgctatcgtc
     2041 tcctatgagc aacgccagaa ttataaggat gacttcaatg cagagtatga tgagtacaga
     2101 gctttgcatg ccaggatgga gactgtagct agaagattta tcaaactaga tgcacaaaga
     2161 aagcgccttt ctccaggctc aaaagagtat cagaatgttc atgaagaagt cttacaagaa
     2221 tatcagaaga taaagcagtc tagtcccaat taccatgaag aaaaatacag atgtgaatat
     2281 cttcataaca agctggctca catcaaaagg ctaataggtg aatttgacca acagcaagca
     2341 gagtcatggt cctagaactc tgcttggacc agaagatgtg aataaactta agcttggaaa
     2401 aaaaaaaaaa aaaaaaaaaa aaaamrcgcg cgtaaccccc cattgcaatg gttgccttaa
     2461 ggtgtttgct agttgtgtac atagtgtggt taatcattag ctacactgct tcccacttga
     2521 ttagagcaat gggaagcata ctgtggccta ccagcatctg gaagtgtgtg ctcgatctgt
     2581 atgtgtgcag aggtggtgtg gatgtgagcg tgcatgaagg aaaaaaagct gctactccta
     2641 gtaggccaaa cgctcaggtt aaacaactga cgagtgttac tgtagggtgt ttttttgttt
     2701 ttttgttttt tttcttctat caaattgcta cttttgttgt ggaagacaaa agcatttcca
     2761 tttcaacgag tttgtcagct ttattaatgt tgggcaaaaa ttgatatgtc atgaaaatga
     2821 aacagatcta tagttttggg acaaaattat aaaatgaaat gtgtaggtaa cctatttata
     2881 tactgctata aagtattttt tgaagagaga tatgcaaaga agctattacc tacataagag
     2941 gtatatttaa agattttttt tttttcatcc tggtgccagg aatataaaaa agagtggata
     3001 tatttaacca taacatactg tgattcatca aacagcacaa actttcattt catggagttt
     3061 atctgttgac attgatttaa actgtcactt gttttatcat gtgggaacat aagttatgtg
     3121 gtcaaaaata taaggatttt gaattaatgt tgattcaagt tgtattgtct tttcaaagtg
     3181 ctgccagttg aaaagggaag cattatgttt acaaatctgt tttgaaatgt ttgccaaaat
     3241 tttggtagtg tctttaataa agatgtttgt ctccagcatc cagaaaaaaa aaaaaaaaan
     3301 ctctccagcg ctggatccgg ccataagggc ctgatccttc gagggggggc ccggtaccag
     3361 cttttgttcc ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt
     3421 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3481 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3541 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     3601 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3661 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3721 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3781 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3841 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3901 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3961 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     4021 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4081 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4141 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4201 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     4261 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4321 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4381 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4441 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     4501 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     4561 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     4621 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4681 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4741 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4801 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4861 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4921 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4981 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     5041 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     5101 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     5161 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     5221 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     5281 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     5341 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     5401 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     5461 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     5521 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     5581 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     5641 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     5701 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     5761 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     5821 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     5881 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     5941 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     6001 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg
     6061 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     6121 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg
     6181 taatacgact cactataggg cgaattggag ctccaccgcg gtggcggccg c
//
