LOCUS       sequence-249689-       12010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-express catalytically inactive mCherry-FLAG-cGAS E225A/D227A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12010)
  AUTHORS   Gentili M, Lahaye X, Nadalin F, Nader GPF, Puig Lombardi E, Herve S,
            De Silva NS, Rookhuizen DC, Zueva E, Goudot C, Maurin M, Bochnakian 
            A, Amigorena S, Piel M, Fachinetti D, Londono-Vallejo A, Manel N
  TITLE     The N-Terminal Domain of cGAS Determines Preferential Association 
            with Centromeric DNA and Innate Immune Activation in the Nucleus.
  JOURNAL   Cell Rep. 2019 Feb 26;26(9):2377-2393.e13. doi: 
            10.1016/j.celrep.2019.01.105.
  PUBMED    30811988
REFERENCE   2  (bases 1 to 12010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.01"; journalName: "Cell Rep"; date: 
            "2019-02-26- 26"; volume: "26"; issue: "9"; pages: "2377-2393.e13"
COMMENT     Sequence Label: pTRIP-CMV-mCherry-FLAG-cGAS E225A/D227A
FEATURES             Location/Qualifiers
     source          1..12010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          31..96
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             226..246
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    671..805
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(708..727)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     762..781
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             973..1606
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1653..1778
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2271..2504
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2689..2733
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3026..3143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3242..3545
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3546..3749
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3700..3720
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3783..4490
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3928..3946)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4196..4215
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4500..4523
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     LTR             6361..6541
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8069..8088)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8302..8321)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8421..8443
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8481..8499)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8567..8671
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8672..8740,8741..9532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8890..8909)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9703..10291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10192..10211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10445..10462
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10532..10552)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10537..10866
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10717..10852
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
       61 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
      121 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
      181 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
      241 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
      301 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
      361 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
      421 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
      481 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
      541 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
      601 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
      661 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
      721 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
      781 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
      841 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
      901 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
      961 aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt
     1021 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     1081 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     1141 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     1201 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     1261 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     1321 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     1381 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     1441 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     1501 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1561 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     1621 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     1681 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     1741 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     1801 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     1861 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     1921 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1981 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     2041 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     2101 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     2161 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     2221 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     2281 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     2341 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     2401 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     2461 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     2521 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     2581 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     2641 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     2701 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     2761 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     2821 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     2881 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     2941 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgccgaatt
     3001 cacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     3061 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     3121 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     3181 tgatacgcgt atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     3241 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     3301 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     3361 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     3421 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     3481 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     3541 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     3601 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     3661 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     3721 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc ccgcgggcta
     3781 gtatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     3841 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     3901 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     3961 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     4021 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     4081 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     4141 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     4201 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     4261 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     4321 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     4381 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     4441 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag actagtatgg
     4501 actacaaaga cgatgacgac aagatgcagc cttggcacgg aaaggccatg cagagagctt
     4561 ccgaggccgg agccactgcc cccaaggctt ccgcacggaa tgccaggggc gccccgatgg
     4621 atcccaacga gtctccggct gcccccgagg ccgccctgcc taaggcggga aagttcggcc
     4681 ccgccaggaa gtcgggatcc cggcagaaaa agagcgcccc ggacacccag gagaggccgc
     4741 ccgtccgcgc aactggggcc cgcgccaaaa aggcccctca gcgcgcccag gacacgcagc
     4801 cgtctgacgc caccagcgcc cctggggcag aggggctgga gcctcctgcg gctcgggagc
     4861 cggctctttc cagggctggt tcttgccgcc agaggggcgc gcgctgctcc acgaagccaa
     4921 gacccccgcc cgggccctgg gacgtgccca gccccggcct gccggtctcg gcccccattc
     4981 tcgtacggag ggatgcggcg cctggggcct cgaagctccg ggcggttttg gagaagttga
     5041 agctcagccg cgatgatatc tccacggcgg cggggatggt gaaaggggtt gtggaccacc
     5101 tgctgctcag actgaagtgc gactccgcgt tcagaggcgt cgggctgctg aacaccggga
     5161 gctactatga gcacgtgaag atttctgcac ctaatgcatt tgctgtcatg tttaaactgg
     5221 aagtccccag aattcaacta gaagaatatt ccaacactcg tgcatattac tttgtgaaat
     5281 ttaaaagaaa tccgaaagaa aatcatctga gtcagttttt agaaggtgaa atattatcag
     5341 cttctaagat gctgtcaaag tttaggaaaa tcattaagga agaaattaac gacattaaag
     5401 atacagatgt catcatgaag aggaaaagag gagggagccc tgctgtaaca cttcttatta
     5461 gtgaaaaaat atctgtggat ataaccctgg ctttggaatc aaaaagtagc tggcctgcta
     5521 gcacccaaga aggcctgcgc attcaaaact ggctttcagc aaaagttagg aagcaactac
     5581 gactaaagcc attttacctt gtacccaagc atgcaaagga aggaaatggt ttccaagaag
     5641 aaacatggcg gctatccttc tctcacatcg aaaaggaaat tttgaacaat catggaaaat
     5701 ctaaaacgtg ctgtgaaaac aaagaagaga aatgttgcag gaaagattgt ttaaaactaa
     5761 tgaaatacct tttagaacag ctgaaagaaa ggtttaaaga caaaaaacat ctggataaat
     5821 tctcttctta tcatgtgaaa actgccttct ttcacgtatg tacccagaac cctcaagaca
     5881 gtcagtggga ccgcaaagac ctgggcctct gctttgataa ctgcgtgaca tactttcttc
     5941 agtgcctcag gacagaaaaa cttgagaatt attttattcc tgaattcaat ctattctcta
     6001 gcaacttaat tgacaaaaga agtaaagaat ttctgacaaa gcaaattgaa tatgaaagaa
     6061 acaatgagtt tccagttttt gatgaatttt gactcgagac ctagaaaaac atggagcaat
     6121 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     6181 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     6241 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     6301 ccaacgaaga caaaatcgtc gagagatgct gcatataagc agctgctttt tgcttgtact
     6361 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6421 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6481 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6541 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     6601 gagagtgaga ggccttgaca ttataataga tttagcagga attgaactag gagtggagca
     6661 cacaggcaaa gctgcagaag tacttggaag aagccaccag agatactcac gattctgcac
     6721 atacctggct aatcccagat cctaaggatt acattaagtt tactaacatt tatataatga
     6781 tttatagttt aaagtataaa cttatctaat ttactattct gacagatatt aattaatcct
     6841 caaatatcat aagagatgat tactattatc cccatttaac acaagaggaa actgagaggg
     6901 aaagatgttg aagtaatttt cccacaatta cagcatccgt tagttacgac tctatgatct
     6961 tctgacacaa attccattta ctcctcaccc tatgactcag tcgaatatat caaagttatg
     7021 gacattatgc taagtaacaa attacccttt tatatagtaa atactgagta gattgagaga
     7081 agaaattgtt tgcaaacctg aatagcttca agaagaagag aagtgaggat aagaataaca
     7141 gttgtcattt aacaagtttt aacaagtaac ttggttagaa agggattcaa atgcataaag
     7201 caagggataa atttttctgg caacaagact atacaatata accttaaata tgacttcaaa
     7261 taattgttgg aacttgataa aactaattaa atattattga agattatcaa tattataaat
     7321 gtaatttact tttaaaaagg gaacatagaa atgtgtatca ttagagtaga aaacaatcct
     7381 tattatcaca atttgtcaaa acaagtttgt tattaacaca agtagaatac tgcattcaat
     7441 taagttgact gcagattttg tgttttgtta aaattagaaa gagataacaa caatttgaat
     7501 tattgaaagt aacatgtaaa tagttctaca tacgttcttt tgacatcttg ttcaatcatt
     7561 gatcgaagtt ctttatcttg gaagaatttg ttccaaagac tctgaaataa ggaaaacaat
     7621 ctattatata gtctcacacc tttgttttac ttttagtgat ttcaatttaa taatgtaaat
     7681 ggttaaaatt tattcttctc tgagatcatt tcacattgca gatagaaaac ctgagactgg
     7741 ggtaattttt attaaaatct aatttaatct cagaaacaca tctttattct aacatcaatt
     7801 tttccagttt gatattatca tataaagtca gccttcctca tctgcaggtt ccacaacaaa
     7861 aatccaacca actgtggatc aaaaatattg ggaaaaaatt aaaaatagca atacaacaat
     7921 aaaaaaatac aaatcagaaa aacagcacag tataacaact ttatttagca tttacaatct
     7981 attaggtatt ataagtaatc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     8041 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     8101 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     8161 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     8221 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     8281 cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     8341 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     8401 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     8461 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     8521 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     8581 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8641 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8701 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     8761 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8821 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8881 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8941 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     9001 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     9061 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     9121 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     9181 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     9241 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     9301 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     9361 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     9421 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     9481 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     9541 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     9601 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     9661 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     9721 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9781 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9841 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9901 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9961 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
    10021 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    10081 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    10141 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    10201 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    10261 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    10321 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
    10381 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
    10441 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
    10501 cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag
    10561 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
    10621 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
    10681 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
    10741 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
    10801 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
    10861 tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc
    10921 cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag
    10981 tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt
    11041 agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt
    11101 gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat
    11161 tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt
    11221 cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa
    11281 gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat
    11341 accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc
    11401 ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat
    11461 acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc
    11521 taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca
    11581 atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac
    11641 cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca
    11701 cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg
    11761 catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt
    11821 ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt
    11881 gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac
    11941 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
    12001 taaatataaa
//
