LOCUS       sequence-249694-       11998 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-express catalytically inactive EGFP-NLS-FLAG-cGAS E225A/D227A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11998)
  AUTHORS   Gentili M, Lahaye X, Nadalin F, Nader GPF, Puig Lombardi E, Herve S,
            De Silva NS, Rookhuizen DC, Zueva E, Goudot C, Maurin M, Bochnakian 
            A, Amigorena S, Piel M, Fachinetti D, Londono-Vallejo A, Manel N
  TITLE     The N-Terminal Domain of cGAS Determines Preferential Association 
            with Centromeric DNA and Innate Immune Activation in the Nucleus.
  JOURNAL   Cell Rep. 2019 Feb 26;26(9):2377-2393.e13. doi: 
            10.1016/j.celrep.2019.01.105.
  PUBMED    30811988
REFERENCE   2  (bases 1 to 11998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.01"; journalName: "Cell Rep"; date: 
            "2019-02-26- 26"; volume: "26"; issue: "9"; pages: "2377-2393.e13"
COMMENT     Sequence Label: pTRIP-SFFV-EGFP-NLS-FLAG- cGAS E225A/D227A
FEATURES             Location/Qualifiers
     source          1..11998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          27..92
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             222..242
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    667..801
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(704..723)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     758..777
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             969..1602
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1649..1774
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2267..2500
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2685..2729
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3022..3139
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3279..3686
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      3720..3729
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3726..3728,3729..3731,3732..4442)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3726..3728,3729..3731,3732..4442)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3771..3792)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4032..4051)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4379..4400
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4464..4484
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4488..4511
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     LTR             6349..6529
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8057..8076)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8290..8309)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8409..8431
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8469..8487)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8555..8659
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8660..8728,8729..9520)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8878..8897)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9691..10279
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10180..10199
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10433..10450
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10520..10540)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10525..10854
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10705..10840
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
       61 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
      121 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
      181 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
      241 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
      301 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
      361 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
      421 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
      481 ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
      541 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
      601 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
      661 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
      721 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
      781 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
      841 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
      901 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
      961 ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat
     1021 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     1081 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     1141 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     1201 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
     1261 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
     1321 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
     1381 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
     1441 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     1501 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     1561 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     1621 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     1681 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     1741 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     1801 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     1861 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     1921 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1981 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     2041 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     2101 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     2161 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     2221 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     2281 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     2341 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     2401 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     2461 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     2521 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     2581 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     2641 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     2701 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     2761 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     2821 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2881 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     2941 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
     3001 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     3061 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     3121 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac tttggctgat
     3181 acgcgtctgc agccccgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     3241 tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt tgcaaggcat
     3301 ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc
     3361 taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag
     3421 aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat
     3481 atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga
     3541 cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc
     3601 gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag
     3661 tcctccgaca gactgagtcg cccgggccgc ggccgcgggc tagcggatcc ccaccggtcg
     3721 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     3781 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     3841 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3901 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     3961 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     4021 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     4081 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     4141 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     4201 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     4261 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     4321 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     4381 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     4441 agactagtgg tggcggcgga agtcccaaga agaagcgcaa ggtgatggac tacaaagacg
     4501 atgacgacaa gatgcagcct tggcacggaa aggccatgca gagagcttcc gaggccggag
     4561 ccactgcccc caaggcttcc gcacggaatg ccaggggcgc cccgatggat cccaacgagt
     4621 ctccggctgc ccccgaggcc gccctgccta aggcgggaaa gttcggcccc gccaggaagt
     4681 cgggatcccg gcagaaaaag agcgccccgg acacccagga gaggccgccc gtccgcgcaa
     4741 ctggggcccg cgccaaaaag gcccctcagc gcgcccagga cacgcagccg tctgacgcca
     4801 ccagcgcccc tggggcagag gggctggagc ctcctgcggc tcgggagccg gctctttcca
     4861 gggctggttc ttgccgccag aggggcgcgc gctgctccac gaagccaaga cccccgcccg
     4921 ggccctggga cgtgcccagc cccggcctgc cggtctcggc ccccattctc gtacggaggg
     4981 atgcggcgcc tggggcctcg aagctccggg cggttttgga gaagttgaag ctcagccgcg
     5041 atgatatctc cacggcggcg gggatggtga aaggggttgt ggaccacctg ctgctcagac
     5101 tgaagtgcga ctccgcgttc agaggcgtcg ggctgctgaa caccgggagc tactatgagc
     5161 acgtgaagat ttctgcacct aatgcatttg ctgtcatgtt taaactggaa gtccccagaa
     5221 ttcaactaga agaatattcc aacactcgtg catattactt tgtgaaattt aaaagaaatc
     5281 cgaaagaaaa tcatctgagt cagtttttag aaggtgaaat attatcagct tctaagatgc
     5341 tgtcaaagtt taggaaaatc attaaggaag aaattaacga cattaaagat acagatgtca
     5401 tcatgaagag gaaaagagga gggagccctg ctgtaacact tcttattagt gaaaaaatat
     5461 ctgtggatat aaccctggct ttggaatcaa aaagtagctg gcctgctagc acccaagaag
     5521 gcctgcgcat tcaaaactgg ctttcagcaa aagttaggaa gcaactacga ctaaagccat
     5581 tttaccttgt acccaagcat gcaaaggaag gaaatggttt ccaagaagaa acatggcggc
     5641 tatccttctc tcacatcgaa aaggaaattt tgaacaatca tggaaaatct aaaacgtgct
     5701 gtgaaaacaa agaagagaaa tgttgcagga aagattgttt aaaactaatg aaataccttt
     5761 tagaacagct gaaagaaagg tttaaagaca aaaaacatct ggataaattc tcttcttatc
     5821 atgtgaaaac tgccttcttt cacgtatgta cccagaaccc tcaagacagt cagtgggacc
     5881 gcaaagacct gggcctctgc tttgataact gcgtgacata ctttcttcag tgcctcagga
     5941 cagaaaaact tgagaattat tttattcctg aattcaatct attctctagc aacttaattg
     6001 acaaaagaag taaagaattt ctgacaaagc aaattgaata tgaaagaaac aatgagtttc
     6061 cagtttttga tgaattttga ctcgagacct agaaaaacat ggagcaatca caagtagcaa
     6121 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     6181 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     6241 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     6301 aaatcgtcga gagatgctgc atataagcag ctgctttttg cttgtactgg gtctctctgg
     6361 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6421 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6481 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     6541 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     6601 ccttgacatt ataatagatt tagcaggaat tgaactagga gtggagcaca caggcaaagc
     6661 tgcagaagta cttggaagaa gccaccagag atactcacga ttctgcacat acctggctaa
     6721 tcccagatcc taaggattac attaagttta ctaacattta tataatgatt tatagtttaa
     6781 agtataaact tatctaattt actattctga cagatattaa ttaatcctca aatatcataa
     6841 gagatgatta ctattatccc catttaacac aagaggaaac tgagagggaa agatgttgaa
     6901 gtaattttcc cacaattaca gcatccgtta gttacgactc tatgatcttc tgacacaaat
     6961 tccatttact cctcacccta tgactcagtc gaatatatca aagttatgga cattatgcta
     7021 agtaacaaat taccctttta tatagtaaat actgagtaga ttgagagaag aaattgtttg
     7081 caaacctgaa tagcttcaag aagaagagaa gtgaggataa gaataacagt tgtcatttaa
     7141 caagttttaa caagtaactt ggttagaaag ggattcaaat gcataaagca agggataaat
     7201 ttttctggca acaagactat acaatataac cttaaatatg acttcaaata attgttggaa
     7261 cttgataaaa ctaattaaat attattgaag attatcaata ttataaatgt aatttacttt
     7321 taaaaaggga acatagaaat gtgtatcatt agagtagaaa acaatcctta ttatcacaat
     7381 ttgtcaaaac aagtttgtta ttaacacaag tagaatactg cattcaatta agttgactgc
     7441 agattttgtg ttttgttaaa attagaaaga gataacaaca atttgaatta ttgaaagtaa
     7501 catgtaaata gttctacata cgttcttttg acatcttgtt caatcattga tcgaagttct
     7561 ttatcttgga agaatttgtt ccaaagactc tgaaataagg aaaacaatct attatatagt
     7621 ctcacacctt tgttttactt ttagtgattt caatttaata atgtaaatgg ttaaaattta
     7681 ttcttctctg agatcatttc acattgcaga tagaaaacct gagactgggg taatttttat
     7741 taaaatctaa tttaatctca gaaacacatc tttattctaa catcaatttt tccagtttga
     7801 tattatcata taaagtcagc cttcctcatc tgcaggttcc acaacaaaaa tccaaccaac
     7861 tgtggatcaa aaatattggg aaaaaattaa aaatagcaat acaacaataa aaaaatacaa
     7921 atcagaaaaa cagcacagta taacaacttt atttagcatt tacaatctat taggtattat
     7981 aagtaatcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
     8041 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
     8101 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
     8161 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
     8221 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg
     8281 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     8341 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     8401 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     8461 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     8521 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     8581 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     8641 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     8701 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     8761 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     8821 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     8881 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     8941 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     9001 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     9061 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     9121 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     9181 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     9241 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     9301 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     9361 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     9421 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     9481 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     9541 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     9601 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9661 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9721 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9781 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     9841 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9901 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9961 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    10021 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    10081 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    10141 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    10201 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    10261 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    10321 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    10381 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    10441 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
    10501 gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
    10561 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
    10621 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
    10681 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    10741 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
    10801 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
    10861 ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg cgtcagggag
    10921 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
    10981 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
    11041 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
    11101 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
    11161 ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat
    11221 accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc
    11281 gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact
    11341 gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg
    11401 gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggattgaa
    11461 cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa
    11521 cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat agtttccagt
    11581 aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc
    11641 ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc
    11701 gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca tgattaaccg
    11761 tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat
    11821 tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga ttggctaccg
    11881 tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct tacttctgtg
    11941 gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaa
//
