LOCUS       sequence-249700-       11966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Over-express catalytically inactive EGFP-FLAG-cGAS E225A/D227A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11966)
  AUTHORS   Gentili M, Lahaye X, Nadalin F, Nader GPF, Puig Lombardi E, Herve S,
            De Silva NS, Rookhuizen DC, Zueva E, Goudot C, Maurin M, Bochnakian 
            A, Amigorena S, Piel M, Fachinetti D, Londono-Vallejo A, Manel N
  TITLE     The N-Terminal Domain of cGAS Determines Preferential Association 
            with Centromeric DNA and Innate Immune Activation in the Nucleus.
  JOURNAL   Cell Rep. 2019 Feb 26;26(9):2377-2393.e13. doi: 
            10.1016/j.celrep.2019.01.105.
  PUBMED    30811988
REFERENCE   2  (bases 1 to 11966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.01"; journalName: "Cell Rep"; date: 
            "2019-02-26- 26"; volume: "26"; issue: "9"; pages: "2377-2393.e13"
COMMENT     Sequence Label: pTRIP-SFFV-EGFP-FLAG-cGAS E225A/D227A
FEATURES             Location/Qualifiers
     source          1..11966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          31..96
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             226..246
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    671..805
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(708..727)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     762..781
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             973..1606
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1653..1778
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2271..2504
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2689..2733
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3026..3143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3283..3690
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      3724..3733
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3730..3732,3733..3735,3736..4446)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3730..3732,3733..3735,3736..4446)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3775..3796)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4036..4055)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4383..4404
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4456..4479
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     LTR             6317..6497
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8025..8044)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8258..8277)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8377..8399
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8437..8455)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8523..8627
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8628..8696,8697..9488)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8846..8865)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9659..10247
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10148..10167
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10401..10418
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10488..10508)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10493..10822
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10673..10808
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
       61 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
      121 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
      181 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
      241 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
      301 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
      361 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
      421 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
      481 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
      541 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
      601 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
      661 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
      721 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
      781 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
      841 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
      901 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
      961 aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt
     1021 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     1081 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     1141 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     1201 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     1261 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     1321 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     1381 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     1441 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     1501 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1561 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     1621 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     1681 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     1741 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     1801 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     1861 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     1921 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     1981 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     2041 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     2101 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     2161 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     2221 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     2281 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     2341 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     2401 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     2461 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     2521 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     2581 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     2641 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     2701 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     2761 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     2821 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     2881 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     2941 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgccgaatt
     3001 cacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     3061 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     3121 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     3181 tgatacgcgt ctgcagcccc gataaaataa aagattttat ttagtctcca gaaaaagggg
     3241 ggaatgaaag accccacctg taggtttggc aagctagctg cagtaacgcc attttgcaag
     3301 gcatggaaaa ataccaaacc aagaatagag aagttcagat caagggcggg tacatgaaaa
     3361 tagctaacgt tgggccaaac aggatatctg cggtgagcag tttcggcccc ggcccggggc
     3421 caagaacaga tggtcaccgc agtttcggcc ccggcccgag gccaagaaca gatggtcccc
     3481 agatatggcc caaccctcag cagtttctta agacccatca gatgtttcca ggctccccca
     3541 aggacctgaa atgaccctgc gccttatttg aattaaccaa tcagcctgct tctcgcttct
     3601 gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc acaacccctc actcggcgcg
     3661 ccagtcctcc gacagactga gtcgcccggg ccgcggccgc gggctagcgg atccccaccg
     3721 gtcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     3781 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     3841 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     3901 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     3961 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     4021 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     4081 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     4141 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     4201 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     4261 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     4321 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     4381 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     4441 tacaagacta gtatggacta caaagacgat gacgacaaga tgcagccttg gcacggaaag
     4501 gccatgcaga gagcttccga ggccggagcc actgccccca aggcttccgc acggaatgcc
     4561 aggggcgccc cgatggatcc caacgagtct ccggctgccc ccgaggccgc cctgcctaag
     4621 gcgggaaagt tcggccccgc caggaagtcg ggatcccggc agaaaaagag cgccccggac
     4681 acccaggaga ggccgcccgt ccgcgcaact ggggcccgcg ccaaaaaggc ccctcagcgc
     4741 gcccaggaca cgcagccgtc tgacgccacc agcgcccctg gggcagaggg gctggagcct
     4801 cctgcggctc gggagccggc tctttccagg gctggttctt gccgccagag gggcgcgcgc
     4861 tgctccacga agccaagacc cccgcccggg ccctgggacg tgcccagccc cggcctgccg
     4921 gtctcggccc ccattctcgt acggagggat gcggcgcctg gggcctcgaa gctccgggcg
     4981 gttttggaga agttgaagct cagccgcgat gatatctcca cggcggcggg gatggtgaaa
     5041 ggggttgtgg accacctgct gctcagactg aagtgcgact ccgcgttcag aggcgtcggg
     5101 ctgctgaaca ccgggagcta ctatgagcac gtgaagattt ctgcacctaa tgcatttgct
     5161 gtcatgttta aactggaagt ccccagaatt caactagaag aatattccaa cactcgtgca
     5221 tattactttg tgaaatttaa aagaaatccg aaagaaaatc atctgagtca gtttttagaa
     5281 ggtgaaatat tatcagcttc taagatgctg tcaaagttta ggaaaatcat taaggaagaa
     5341 attaacgaca ttaaagatac agatgtcatc atgaagagga aaagaggagg gagccctgct
     5401 gtaacacttc ttattagtga aaaaatatct gtggatataa ccctggcttt ggaatcaaaa
     5461 agtagctggc ctgctagcac ccaagaaggc ctgcgcattc aaaactggct ttcagcaaaa
     5521 gttaggaagc aactacgact aaagccattt taccttgtac ccaagcatgc aaaggaagga
     5581 aatggtttcc aagaagaaac atggcggcta tccttctctc acatcgaaaa ggaaattttg
     5641 aacaatcatg gaaaatctaa aacgtgctgt gaaaacaaag aagagaaatg ttgcaggaaa
     5701 gattgtttaa aactaatgaa atacctttta gaacagctga aagaaaggtt taaagacaaa
     5761 aaacatctgg ataaattctc ttcttatcat gtgaaaactg ccttctttca cgtatgtacc
     5821 cagaaccctc aagacagtca gtgggaccgc aaagacctgg gcctctgctt tgataactgc
     5881 gtgacatact ttcttcagtg cctcaggaca gaaaaacttg agaattattt tattcctgaa
     5941 ttcaatctat tctctagcaa cttaattgac aaaagaagta aagaatttct gacaaagcaa
     6001 attgaatatg aaagaaacaa tgagtttcca gtttttgatg aattttgact cgagacctag
     6061 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     6121 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     6181 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6241 aagggctaat tcactcccaa cgaagacaaa atcgtcgaga gatgctgcat ataagcagct
     6301 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6361 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6421 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6481 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     6541 aaagaaatga atatcagaga gtgagaggcc ttgacattat aatagattta gcaggaattg
     6601 aactaggagt ggagcacaca ggcaaagctg cagaagtact tggaagaagc caccagagat
     6661 actcacgatt ctgcacatac ctggctaatc ccagatccta aggattacat taagtttact
     6721 aacatttata taatgattta tagtttaaag tataaactta tctaatttac tattctgaca
     6781 gatattaatt aatcctcaaa tatcataaga gatgattact attatcccca tttaacacaa
     6841 gaggaaactg agagggaaag atgttgaagt aattttccca caattacagc atccgttagt
     6901 tacgactcta tgatcttctg acacaaattc catttactcc tcaccctatg actcagtcga
     6961 atatatcaaa gttatggaca ttatgctaag taacaaatta cccttttata tagtaaatac
     7021 tgagtagatt gagagaagaa attgtttgca aacctgaata gcttcaagaa gaagagaagt
     7081 gaggataaga ataacagttg tcatttaaca agttttaaca agtaacttgg ttagaaaggg
     7141 attcaaatgc ataaagcaag ggataaattt ttctggcaac aagactatac aatataacct
     7201 taaatatgac ttcaaataat tgttggaact tgataaaact aattaaatat tattgaagat
     7261 tatcaatatt ataaatgtaa tttactttta aaaagggaac atagaaatgt gtatcattag
     7321 agtagaaaac aatccttatt atcacaattt gtcaaaacaa gtttgttatt aacacaagta
     7381 gaatactgca ttcaattaag ttgactgcag attttgtgtt ttgttaaaat tagaaagaga
     7441 taacaacaat ttgaattatt gaaagtaaca tgtaaatagt tctacatacg ttcttttgac
     7501 atcttgttca atcattgatc gaagttcttt atcttggaag aatttgttcc aaagactctg
     7561 aaataaggaa aacaatctat tatatagtct cacacctttg ttttactttt agtgatttca
     7621 atttaataat gtaaatggtt aaaatttatt cttctctgag atcatttcac attgcagata
     7681 gaaaacctga gactggggta atttttatta aaatctaatt taatctcaga aacacatctt
     7741 tattctaaca tcaatttttc cagtttgata ttatcatata aagtcagcct tcctcatctg
     7801 caggttccac aacaaaaatc caaccaactg tggatcaaaa atattgggaa aaaattaaaa
     7861 atagcaatac aacaataaaa aaatacaaat cagaaaaaca gcacagtata acaactttat
     7921 ttagcattta caatctatta ggtattataa gtaatctagc cagatcctct acgccggacg
     7981 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
     8041 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
     8101 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt
     8161 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga
     8221 gtcgcataag ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat
     8281 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     8341 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     8401 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     8461 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     8521 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8581 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8641 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8701 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8761 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8821 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8881 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8941 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     9001 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     9061 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     9121 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     9181 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     9241 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     9301 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     9361 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9421 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9481 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9541 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9601 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9661 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9721 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9781 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9841 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9901 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9961 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    10021 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    10081 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    10141 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    10201 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    10261 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    10321 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    10381 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    10441 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca
    10501 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    10561 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    10621 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
    10681 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
    10741 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
    10801 tggaggccta ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga
    10861 gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg
    10921 aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga
    10981 acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg
    11041 tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg
    11101 aacaatggaa aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc
    11161 gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa
    11221 ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat
    11281 tgatgacctg gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc
    11341 gcactttgtc accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt
    11401 tgatatcccg caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc
    11461 gccaatctcc ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa
    11521 cttcaggcgg gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg
    11581 caaacctgag cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag
    11641 tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca
    11701 tccctcactg gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac
    11761 gcgaaatcct cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg
    11821 atttatacga tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg
    11881 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag
    11941 atttaaagct ctaaggtaaa tataaa
//
