LOCUS       sequence-249726-        4440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes human NFATC2 as a Type 3b part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3b_024
FEATURES             Location/Qualifiers
     source          1..4440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     328..347
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      595..1183
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1084..1103
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     regulatory      3461..3470
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      complement(4089..4123)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(4194..4440,1..413))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
       61 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
      121 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
      181 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
      241 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
      301 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
      361 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
      421 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
      481 ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg
      541 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
      601 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
      661 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
      721 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
      781 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
      841 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
      901 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
      961 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     1021 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     1081 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     1141 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     1201 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     1261 ccctgattct gtggataacc gtagtcggtc tcattctaac gcccccgagc ggcagcccca
     1321 acccgacggc ggggacgccc caggccacga gcctgggggc agcccccaag acgagcttga
     1381 cttctccatc ctcttcgact atgagtattt gaatccgaac gaagaagagc cgaatgcaca
     1441 taaggtcgcc agcccaccct ccggacccgc ataccccgat gatgtcctgg actatggcct
     1501 caagccatac agcccccttg ctagtctctc tggcgagccc cccggccgat tcggagagcc
     1561 ggatagggta gggccgcaga agtttctgag cgcggccaag ccagcagggg cctcgggcct
     1621 gagccctcgg atcgagatca ctccgtccca cgaactgatc caggcagtgg ggcccctccg
     1681 catgagagat gcgggcctcc tggtggagca gccgcccctg gccggggtgg ccgccagccc
     1741 gaggttcacc ctgcccgtgc ccggcttcga gggctaccgc gagccgcttt gcttgagccc
     1801 cgctagcagc ggctcctctg ccagcttcat ttctgacacc ttctccccct acacctcgcc
     1861 ctgtgtctcg cccaataacg gcgggcccga cgacctgtgt ccgcagtttc aaaacatccc
     1921 tgctcattat tcccccagaa cctcgccaat aatgtcacct cgaaccagcc tcgccgagga
     1981 cagctgcctg ggccgccact cgcccgtgcc ccgtccggcc tcccgctcct catcgcctgg
     2041 tgccaagcgg aggcattcgt gcgccgaggc cttggttgcc ctgccgcccg gagcctcacc
     2101 ccagcgctcc cggagcccct cgccgcagcc ctcatctcac gtggcacccc aggaccacgg
     2161 ctccccggct gggtaccccc ctgtggctgg ctctgccgtg atcatggatg ccctgaacag
     2221 cctcgccacg gactcgcctt gtgggatccc ccccaagatg tggaagacca gccctgaccc
     2281 ctcgccggtg tctgccgccc catccaaggc cggcctgcct cgccacatct acccggccgt
     2341 ggagttcctg gggccctgcg agcagggcga gaggagaaac tcggctccag aatccatcct
     2401 gctggttccg cccacttggc ccaagccgct ggtgcctgcc attcccatct gcagcatccc
     2461 agtgactgca tccctccctc cacttgagtg gccgctgtcc agtcagtcag gctcttacga
     2521 gctgcggatc gaggtgcagc ccaagccaca tcaccgggcc cactatgaga cagaaggcag
     2581 ccgaggggct gtcaaagctc caactggagg ccaccctgtg gttcagctcc atggctacat
     2641 ggaaaacaag cctctgggac ttcagatctt cattgggaca gctgatgagc ggatccttaa
     2701 gccgcacgcc ttctaccagg tgcaccgaat cacggggaaa actgtcacca ccaccagcta
     2761 tgagaagata gtgggcaaca ccaaagtcct ggagataccc ttggagccca aaaacaacat
     2821 gagggcaacc atcgactgtg cggggatctt gaagcttaga aacgccgaca ttgagctgcg
     2881 gaaaggcgaa acggacattg gaagaaagaa cacgcgggtg agactggttt tccgagttca
     2941 catcccagag tccagtggca gaattgtctc tttacagact gcatctaacc ccatcgagtg
     3001 ctcccagcga tctgctcacg agctgcccat ggttgaaaga caagacacag acagctgcct
     3061 ggtctatggc ggccagcaaa tgatcctcac ggggcagaac tttacatccg agtccaaagt
     3121 tgtgtttact gagaagacca cagatggaca gcaaatttgg gagatggaag ccacggtgga
     3181 taaggacaag agccagccca acatgctttt tgttgagatc cctgaatatc ggaacaagca
     3241 tatccgcaca cctgtaaaag tgaacttcta cgtcatcaat gggaagagaa aacgaagtca
     3301 gcctcagcac tttacctacc acccagtccc agccatcaag acggagccca cggatgaata
     3361 tgaccccact ctgatctgca gccccaccca tggaggcctg gggagccagc cttactaccc
     3421 ccagcacccg atggtggccg agtccccctc ctgcctcgtg gccaccatgg ctccctgcca
     3481 gcagttccgc acggggctct catcccctga cgcccgctac cagcaacaga acccagcggc
     3541 cgtactctac cagcggagca agagcctgag ccccagcctg ctgggctatc agcagccggc
     3601 cctcatggcc gccccgctgt cccttgcgga cgctcaccgc tctgtgctgg tgcacgccgg
     3661 ctcccagggc cagagctcag ccctgctcca cccctctccg accaaccagc aggcctcgcc
     3721 tgtgatccac tactcaccca ccaaccagca gctgcgctgc ggaagccacc aggagttcca
     3781 gcacatcatg tactgcgaga atttcgcacc aggcaccacc agacctggcc cgcccccggt
     3841 cagtcaaggt cagaggctga gcccgggttc ctaccccaca gtcattcagc agcagaatgc
     3901 cacgagccaa agagccgcca aaaacggacc cccggtcagt gaccaaaagg aagtattacc
     3961 tgcgggggtg accattaaac aggagcagaa cttggaccag acctacttgg atgatgttaa
     4021 tgaaattatc aggaaggagt tttcaggacc tcctgccaga aatcagacgg gatcctgaga
     4081 ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
     4141 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
     4201 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     4261 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     4321 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     4381 aaatcaaaac tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata
//
