LOCUS       sequence-249727-        2844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes human TP53 as a Type 3b part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3b_027
FEATURES             Location/Qualifiers
     source          1..2844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      131..719
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     620..639
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             834..2009
                     /codon_start=1
                     /gene="Homo sapiens TP53"
                     /product="human tumor suppressor protein that is often 
                     mutated in cancers"
                     /label=p53
                     /note="responds to cellular stresses to regulate the 
                     expression of target genes"
                     /translation="EEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLS
                     PDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQG
                     SYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYK
                     QSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPP
                     EVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDR
                     RTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMF
                     RELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD"
     terminator      complement(2029..2063)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2134..2793)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2708..2727
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga
       61 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
      121 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      181 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      241 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      301 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      361 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      421 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      481 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      541 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      601 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      661 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      721 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
      781 tctttcctgc gttatcccct gattctgtgg ataaccgtag tcggtctcat tctgaggagc
      841 cgcagtcaga tcctagcgtc gagccccctc tgagtcagga aacattttca gacctatgga
      901 aactacttcc tgaaaacaac gttctgtccc ccttgccgtc ccaagcaatg gatgatttga
      961 tgctgtcccc ggacgatatt gaacaatggt tcactgaaga cccaggtcca gatgaagctc
     1021 ccagaatgcc agaggctgct ccccccgtgg cccctgcacc agcagctcct acaccggcgg
     1081 cccctgcacc agccccctcc tggcccctgt catcttctgt cccttcccag aaaacctacc
     1141 agggcagcta cggtttccgt ctgggcttct tgcattctgg gacagccaag tctgtgactt
     1201 gcacgtactc ccctgccctc aacaagatgt tttgccaact ggcaaagacc tgccctgtgc
     1261 agctgtgggt tgattccaca cccccgcccg gcacccgcgt ccgcgccatg gccatctaca
     1321 agcagtcaca gcacatgacg gaggttgtga ggcgctgccc ccaccatgag cgctgctcag
     1381 atagcgatgg tctggcccct cctcagcatc ttatccgagt ggaaggaaat ttgcgtgtgg
     1441 agtatttgga tgacagaaac acttttcgac atagtgtggt ggtgccctat gagccgcctg
     1501 aggttggctc tgactgtacc accatccact acaactacat gtgtaacagt tcctgcatgg
     1561 gcggcatgaa ccggaggccc atcctcacca tcatcacact ggaagactcc agtggtaatc
     1621 tactgggacg gaacagcttt gaggtgcgtg tttgtgcctg tcctgggaga gatcggcgca
     1681 cagaggaaga gaatctccgc aagaaagggg agcctcacca cgagctgccc ccagggagca
     1741 ctaagcgagc actgcccaac aacaccagct cctctcccca gccaaagaag aaaccactgg
     1801 atggagaata tttcaccctt cagatccgtg ggcgtgagcg cttcgagatg ttccgagagc
     1861 tgaatgaggc cttggaactc aaggatgccc aggctgggaa ggagccaggg gggagcaggg
     1921 ctcactccag ccacctgaag tccaaaaagg gtcagtctac ctcccgccat aaaaaactca
     1981 tgttcaagac agaagggcct gactcagacg gatcctgaga ccagaccaat aaaaaacgcc
     2041 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     2101 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca
     2161 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
     2221 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     2281 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     2341 cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc
     2401 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     2461 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     2521 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
     2581 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     2641 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     2701 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     2761 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     2821 cgataactca aaaaatacgc ccgg
//
