LOCUS       sequence-249732-        2379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Venus as a Type 3b part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK3b_028
FEATURES             Location/Qualifiers
     source          1..2379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             14..724
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     ICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     terminator      complement(744..778)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(849..1508)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1423..1442
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1690..2278
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2179..2198
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat tcttctaaag gtgaagaatt attcactggt gttgtcccaa ttttggttga
       61 attagatggt gatgttaatg gtcacaaatt ttctgtctcc ggtgaaggtg aaggtgatgc
      121 tacttacggt aaattgacct taaaattgat ttgtactact ggtaaattgc cagttccatg
      181 gccaacctta gtcactactt taggttatgg tttgcaatgt tttgctagat acccagatca
      241 tatgaaacaa catgactttt tcaagtctgc catgccagaa ggttatgttc aagaaagaac
      301 tatttttttc aaagatgacg gtaactacaa gaccagagct gaagtcaagt ttgaaggtga
      361 taccttagtt aatagaatcg aattaaaagg tattgatttt aaagaaggtg gtaacatttt
      421 aggtcacaaa ttggaataca actataactc tcacaatgtt tacatcactg ctgacaaaca
      481 aaagaatggt atcaaagcta acttcaaaat tagacacaac attgaagatg gtggtgttca
      541 attagctgac cattatcaac aaaatactcc aattggtgat ggtccagtct tgttaccaga
      601 caaccattac ttatcctatc aatctgcctt atccaaagat ccaaacgaaa agagagatca
      661 catggtcttg ttagaatttg ttactgctgc tggtattacc catggtatgg atgaattgta
      721 caaaggatcc tgagaccaga ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
      781 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg
      841 atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
      901 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
      961 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     1021 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgaaacgaaa
     1081 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     1141 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     1201 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     1261 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
     1321 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     1381 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     1441 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     1501 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     1561 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat caatcatgac
     1621 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1681 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1741 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1801 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     1861 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1921 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1981 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2041 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2101 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2161 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2221 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2281 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2341 ctttcctgcg ttatcccctg attctgtgga taaccgtag
//
