LOCUS       sequence-249756-        1671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes spacer sequence (multicistronic) as a Type 4 part to be used
            in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK4_006
FEATURES             Location/Qualifiers
     source          1..1671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     209..228
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(445..479)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(550..1209)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1124..1143
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1391..1671,1..308)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
       61 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      121 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      181 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      241 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      301 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      361 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtagt cggtctcaat
      421 ccggtgggct gtgagaccag accaataaaa aacgcccggc ggcaaccgag cgttctgaac
      481 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
      541 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat
      601 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag
      661 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc
      721 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa
      781 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
      841 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
      901 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
      961 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc
     1021 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     1081 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     1141 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     1201 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     1261 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga
     1321 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1381 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1441 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1501 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1561 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1621 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca a
//
