LOCUS       sequence-249758-       12437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses gRNA in Aedes aegypti.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12437)
  AUTHORS   Li M, Yang T, Kandul NP, Bui M, Gamez S, Raban R, Bennett J, Sanchez
            C HM, Lanzaro GC, Schmidt H, Lee Y, Marshall JM, Akbari OS
  TITLE     Development of a confinable gene drive system in the human disease 
            vector Aedes aegypti.
  JOURNAL   Elife. 2020 Jan 21;9. pii: 51701. doi: 10.7554/eLife.51701.
  PUBMED    31960794
REFERENCE   2  (bases 1 to 12437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-01-21"; volume: "9. pii: 51701. doi"; pages: " 
            10.7554/eLife.51701"
COMMENT     Sequence Label: U6d
FEATURES             Location/Qualifiers
     source          1..12437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..681)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     polyA_signal    795..916
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   1584..1618
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(1799..1815)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1799..1815)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1812..1834)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1823..1839
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1847..1853,1854..1871,1872..1877))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1892..1913
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2030..2047)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2201..2789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2281..2300)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2960..3751,3752..3820))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3583..3602
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3821..3925)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3993..4011
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4049..4071)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4171..4190
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4384..4406
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4398..4415
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4399..4415
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   5119..5181
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      9017..9026
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    10575..10696
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 atggtgcgct cctccaagaa cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag
       61 ggcaccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      121 ggccacaaca ccgtgaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
      181 atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc cgccgacatc
      241 cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      301 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg ctgcttcatc
      361 tacaaggtga agttcatcgg cgtgaacttc ccctccgacg gccccgtaat gcagaagaag
      421 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
      481 gagatccaca aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagtcc
      541 atctacatgg ccaagaagcc cgtgcagctg cccggctact actacgtgga ctccaagctg
      601 gacatcacct cccacaacga ggactacacc atcgtggagc agtacgagcg caccgagggc
      661 cgccaccacc tgttcctgta ggactctaga tcataatcag ccataccaca tttgtagagg
      721 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
      781 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
      841 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      901 tcatcaatgt atcttaaagc ttctaggccg gccgatctcg gatctgacaa tgttcagtgc
      961 agagactcgg ctacgcctcg tggactttga agttgaccaa caatgtttat tcttacctct
     1021 aatagtcctc tgtggcaagg tcaagattct gttagaagcc aatgaagaac ctggttgttc
     1081 aataacattt tgttcgtcta atatttcact accgcttgac gttggctgca cttcatgtac
     1141 ctcatctata aacgcttctt ctgtatcgct ctggacgtca tcttcactta cgtgatctga
     1201 tatttcactg tcagaatcct caccaacaag ctcgtcatcg ctttgcagaa gagcagagag
     1261 gatatgctca tcgtctaaag aactacccat tttattatat attagtcacg atatctataa
     1321 caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt
     1381 atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac
     1441 gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga
     1501 gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa
     1561 gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt
     1621 aaaaaagatt tgcgctttac tcgacctaaa ctttaaacac gtcatagaat cttcgtttga
     1681 caaaaaccac attgtggcca agctgtgtga cgcgacgcgc gctaaagaat ggcaaaccaa
     1741 gtcgcgcgag cgtcgactct agaggatccc cgggtaccga gctcgaattc gtaatcatgt
     1801 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     1861 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     1921 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     1981 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     2041 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     2101 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2161 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2221 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2281 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2341 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2401 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2461 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2521 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2581 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2641 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2701 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2761 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2821 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2881 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2941 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     3001 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     3061 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     3121 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3181 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3241 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     3301 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3361 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3421 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3481 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     3541 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     3601 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3661 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3721 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3781 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3841 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3901 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     3961 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     4021 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     4081 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     4141 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     4201 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     4261 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     4321 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     4381 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctttgttta aaaatataac
     4441 aaaattgtga tcccacaaaa tgaagtgggg caaaatcaaa taattaatag tgtccgtaaa
     4501 cttgttggtc ttcaactttt tgaggaacac gttggacggc aaatccgtga ctataacaca
     4561 agttgattta ataattttag ccaacacgtc gggctgcgtg ttttttgccg acgcgtctgt
     4621 gtacacgttg attaactggt cgattaaact gttgaaataa tttaattttt ggttcttctt
     4681 taaatctgtg atgaaatttt ttaaaataac tttaaattct tcattggtaa aaaatgccac
     4741 gttttgcaac ttgtgagggt ctaatatgag gtcaaactca gtaggagttt tatccaaaaa
     4801 agaaaacatg attacgtctg tacacgaacg cgtattaacg cagagtgcaa agtataagag
     4861 ggttaaaaaa tatattttac gcaccatata cgcatcgggt tgatatcgtt aatatggatc
     4921 aatttgaaca gttgattaac gtgtctctgc tcaagtcttt gatcaaaacg caaatcgacg
     4981 aaaatgtgtc ggacaatatc aagtcgatga gcgaaaaact aaaaaggcta gaatacgaca
     5041 atctcacaga cagcgttgag atatacggta ttcacgacag caggctgaat aataaaaaaa
     5101 ttagaaacta ttatttaacc ctagaaagat aatcatattg tgacgtacgt taaagataat
     5161 catgcgtaaa attgacgcat gtgttttatc ggtctgtata tcgaggttta tttattaatt
     5221 tgaatagata ttaagtttta ttatatttac acttacatac taataataaa ttcaacaaac
     5281 aatttattta tgtttattta tttattaaaa aaaaacaaaa actcaaaatt tcttctataa
     5341 agtaacaaaa cttttaaaca ttctctcttt tacaaaaata aacttatttt gtactttaaa
     5401 aacagtcatg ttgtattata aaataagtaa ttagcttaac ttatacataa tagaaacaaa
     5461 ttatacttat tagtcagtca gaaacaactt tggcacatat caatattatg ctctcgacaa
     5521 ataacttttt tgcatttttt gcacgatgca tttgcctttc gccttatttt agaggggcag
     5581 taagtacagt aagtacgttt tttcattact ggctcttcag tactgtcatc tgatgtacca
     5641 ggcacttcat ttggcaaaat attagagata ttatcgcgca aatatctctt caaagtagga
     5701 gcttctaaac gcttacgcat aaacgatgac gtcaggctca tgtaaaggtt tctcataaat
     5761 tttttgcgac tttgaacctt ttctcccttg ctactgacat tatggctgta tataataaaa
     5821 gaatttatgc aggcaatgtt tatcattccg tacaataatg ccataggcca cctattcgtc
     5881 ttcctactgc aggtcatcac agaacacatt tggtctagcg tgtccactcc gcctttagtt
     5941 tgattataat acataaccat ttgcggttta ccggtacttt cgttgataga agcatcctca
     6001 tcacaagatg ataataagta taccatctta gctggcttcg gtttatatga gacgagagta
     6061 aggggtccgt caaaacaaaa catcgatgtt cccactggcc tggagcgact gtttttcagt
     6121 acttccggta tctcgcgttt gtttgatcgc acggttccca caatggttaa ttcgagctcg
     6181 cccggggtcc taggcctcac gccactcgta gaatgaaccc gattgaatca tttttaaacc
     6241 gaaaaaaaaa tcctgccggt gttctctgag ttttctgagg attctgatcg ggatcccaga
     6301 ggaacctggg aattgctatt ggattcctaa cagactcttg agaattacca tcagagtttc
     6361 gtacattttt aacaagattt tttgtggaat catcaccaaa ccatgaaaat ttctagcagg
     6421 cttttggggt tcctcagtgg gatcatgaaa ctccgttaca tcgtttttgc tatgagatta
     6481 cagaaaaaat aacttaagta aacagttttt ctgtaaaaga tatttcactc acttagcttt
     6541 tgaacgcagt ggaattttag accattgttt gcttgacttt ttttttaatc ataatttaaa
     6601 tgttagagtg ttagaaaccg tgttcttata aacaatattc tccttttctt cttccctgtc
     6661 ttgcgaaaca aacgatagag agaaacttat cagtgtacct ggcgtctaag ctgcgaatca
     6721 aggaactacg attttccagc cacttcctat gtaaatcaat cgaagtatcg tttagtacat
     6781 tacatttaat acatttcttc ttcatctatg catctttgac ttttccaaaa tgaatatcct
     6841 atttcattat acattttttc ttttacttcc agcgctacag caccagcagt tatcaggatg
     6901 tagatgaagg tatcaacagt tcgttcggca gcaatgacaa atccacgctg attcaggttt
     6961 ggaaacccaa aagttatggc gccgtcaagg gacaaattcc acagcacgaa cgactcacat
     7021 acacatggaa ggagatcgat gtgtttggag agacgccagg tgataccaaa aaggaacccc
     7081 tgtgcagtag attgtgctgt tgtttctccc gccagaagaa agacttcaat ccccgcaaac
     7141 acctactgaa gaatgtgacc gggatggcta agagtggaga gttactggct gtgatgggta
     7201 gttctggcgc cggtaaaaca accttgctga acgctttgct agctcattca tcttagttat
     7261 atactcaatg gaaacggtaa aagttctagc gatgggcgaa aatatttttt cctatccata
     7321 aagagagctt taagttagtc ctctttgacc tttttattat aatatttgat ttgacctcgt
     7381 tggctttcaa tgacattctt tgagagaaca atgagtatct taaacgctca aataaatatt
     7441 acctatcgca tctcttcata gtaaaataaa tgttccaaac agatccgtgt ttgcgattgc
     7501 atatttcaag gcgtaatgta tttttcggag gatggatgct gacaatgaaa ccgtatccgt
     7561 tatcaccatg gagaggtgtt gaagaacgaa caattaagaa gaaagttttt tgttttcggc
     7621 atttgatagg atcttaccac tcgaagccaa tccagcgaga caaatagctt gaagttattt
     7681 agcgcatcgt taaattttta acgactctaa ttggagctgc cagacaaatt tgatttgtcc
     7741 gtgcggtatg catatgtacc tacttacgtc tgtcgttttg tctccgttta ccgggaggga
     7801 aagtctggaa acatggaaac tctatagttg ccaggtagac catctgcctc cgtcggctgg
     7861 ctggattcca atttgaatat tggctaattg gaagagatgg aagtttttga atggatgatt
     7921 gaataattga agcgactccg ggtacctgtt tgtaagctct gcaacagtgc catagattcg
     7981 tgtcagtcca tcactagaat caaatcaact tgtacttgat atataaatgg cttgggttaa
     8041 tggtgcgatc ttgacccccg ggtcttagag ctatgctgga aacagcatag caagttaaga
     8101 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttacaaat
     8161 ctattatgca tgaggtacta tgttggcaga gtaatgaatg cctaaaaaaa tatatgaaca
     8221 ttaatatggc cttaaaaaga actgtctata tcttcaaaat actgcaacca tcaaacccaa
     8281 acatagaatg aacctattta agtaaagtag ctatcatttg aaacctacga aatcaatgaa
     8341 tttgttgaat tattatttga cctataagat tctgtacgga aaattcttaa atatgtttaa
     8401 acatcttatt ttaaattata atgtgttcat gtcttcttct ctcttttcag gtacttacaa
     8461 cagcgttatg aaatcctgtt ctgatacggt atgttttggt ttgttcgttc taaagtgaca
     8521 gttttctttt aaatgctttc atgaactaat ttaaagcgat ggagcttaga aaacaatttc
     8581 cgaatctata aagcattttg tttgcagaag ttgacacaag tagaatctgt gttttgactg
     8641 tataaaagtt ttcccctcgt ttttccttat tattatttcc tccatttcct tggccgtcac
     8701 actgcatttc cggtgcggca aagtcgggcg acgacgacca ccacaggtgt tcttaattaa
     8761 ttcgagctcg cccggggatc taattcaatt agagactaat tcaattagag ctaattcaat
     8821 taggatccaa gcttatcgat ttcgaaccct cgaccgccgg agtataaata gaggcgcttc
     8881 gtctacggag cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct
     8941 aagcaaataa acaagcgcag ctgaacaagc taaacaatcg gggtaccgct agagtcgacg
     9001 gtacgatcca ccggtcgcca ccatggtgag caagggcgag gaggtcatca aagagttcat
     9061 gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga
     9121 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
     9181 ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta
     9241 cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa
     9301 gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc
     9361 cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga
     9421 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc
     9481 ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca
     9541 ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta
     9601 ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     9661 acagtacgag cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac
     9721 cggcagcggc agctccggca ccgcctcctc cgaggacaac aacatggccg ttatcaagga
     9781 atttatgcgc ttcaaagtta ggatggaggg atccatgaac ggacatgagt tcgagatcga
     9841 gggagagggc gagggacgcc cgtatgaagg cacacaaaca gccaaactca aggtcaccaa
     9901 gggcggacca ctgcccttcg cctgggatat cctgagtccc cagtttatgt acggcagcaa
     9961 ggcctacgtt aagcaccccg ctgacatacc ggactacaaa aagctgtcct ttccggaagg
    10021 cttcaagtgg gagcgcgtga tgaatttcga agacggagga ctggtcactg tgacccaaga
    10081 tagcagtttg caggacggta cactgatcta taaggttaaa atgcgcggca ctaactttcc
    10141 gccagatggc ccagtgatgc agaagaagac catgggttgg gaggcatcca ccgaacgtct
    10201 gtaccctcga gacggagtgc tcaagggcga gatccatcag gccctcaaac tgaaagatgg
    10261 tggtcactac ctggtcgaat ttaagaccat ttacatggcc aagaagccgg ttcagctgcc
    10321 cggatattat tatgtggata cgaaactgga tataacttcg cataacgaag actacaccat
    10381 tgtcgagcag tatgagcgca gcgaaggccg acatcacctg ttcctctacg gcatggacga
    10441 gctgtacaag taggcggccg cgactctaga tcataatcag ccataccaca tttgtagagg
    10501 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
    10561 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    10621 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    10681 tcatcaatgt atcttaaagc ttgtttaaac tcaagatcgc accgacttca gtacgtgccc
    10741 taaacggaat cccagtcaac gcggaacagt tgcgagcacg ttgcgcctat gtgcaacagg
    10801 acgatctttt cattcccgcg cttacaactc gagaacacct cgtattccac gcaatgctgc
    10861 gtatgggtaa agatgtaccc aagagtgtca agatgaatcg agtcaacgag gtgcttcagg
    10921 agctttcctt agcgaaatgc gcagacacga tcattggagc gccaggtcga atgaaaggcc
    10981 tgtccggagg tgaacggaaa cggttggcct ttgcatcgga aaccctgacc gatcctcacc
    11041 tgttgctttg tgatgaaccc acttccgggt tggattcgtt catggctcac agtgttctac
    11101 aggtgctgaa gggaatggcc ctcaaaggaa agacgattat actgaccata catcaacctt
    11161 cgtcagaact gtattgcttg ttcgacaaaa ttctcctagt ggcagaagga cgcgtggctt
    11221 tccttggatc tccctatcag gcttcggaat tcttttctca gtgagtagac ttgtgaaatc
    11281 ctgacgattt tgctttacta gcagggcttg tggtgtcttg cctcttaaaa caccgaacat
    11341 gaaacccaaa agccaaacgc aataggaatc agacgatgag agttcttcat agatctgacc
    11401 agacatgtct ctaagaatat ttctaagact ttttttttgg aaattcatcg cgatcttgac
    11461 attacgcgta atccgcgtaa tcatcgtatt tttccaaagt tacattcaga tattacggca
    11521 gaaattcttt aggagatttc atcaggagat aacatattgg tgaattacgg attcgttgta
    11581 tttatcttca agaatactac ctgattttag tttagcaatt acttcagaga tttcatttag
    11641 aattcaagat tcatgcagaa atattctttc attgatagaa cttccttcta tgcagaacat
    11701 ctaccagaat tcaaccagaa atttctactt atatacctct aacacggcca attgcggcca
    11761 gacagtaaaa taatagtggc cttagtaact aaaatgatca aaattgtgac ccggtaaagt
    11821 gatttaataa aacagttttt agtccaaaca atattagggg tcatccaaaa acgacttcaa
    11881 tcattagggg cgcgcccata ctcggtggcc tccccaccac caactttttt gcactgcaaa
    11941 aaaacacgct tttgcacgcg ggcccataca tagtacaaac tctacgtttc gtagactatt
    12001 ttacataaat agtctacacc gttgtatacg ctccaaatac actaccacac attgaacctt
    12061 tttgcagtgc aaaaaagtac gtgtcggcag tcacgtaggc cggccttatc gggtcgcgtc
    12121 ctgtcacgta cgaatcacat tatcggaccg gacgagtgtt gtcttatcgt gacaggacgc
    12181 cagcttcctg tgttgctaac cgcagccgga cgcaactcct tatcggaaca ggacgcgcct
    12241 ccatatcagc cgcgcgttat ctcatgcgcg tgaccggaca cgaggcgccc gtcccgctta
    12301 tcgcgcctat aaatacagcc cgcaacgatc tggtaaacac agttgaacag catctgttac
    12361 agcgacacaa catgagccgg tccaacaacg ccaacgcgcc cacgccatcc aaccgccgcc
    12421 gcaacctgtc tctggtg
//
