LOCUS       sequence-249761-        1705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Nuclear import sequence as a Type 4a part to be used in the 
            MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK4a_003
FEATURES             Location/Qualifiers
     source          1..1705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(69..657)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(149..168)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             839..1498
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(905..924)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1569..1603
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1639..1659)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
       61 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      121 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      181 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      241 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      301 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      361 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      421 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      481 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
      541 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
      601 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
      661 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
      721 attttggtca tgattgatcg ggcacgtaag aggttccaac tttcaccata atgaaataag
      781 atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat
      841 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
      901 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat
      961 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
     1021 cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga aagacggtga
     1081 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
     1141 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
     1201 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
     1261 tatgtttttc gtttcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
     1321 caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga
     1381 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt
     1441 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt
     1501 tgatatcgag ctcgcttgga ctcctgttga tagatccagt aatgacctca gaactccatc
     1561 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtctg gtctcagcca
     1621 ctcgagttag gcgtcctcca ccttcctctt cttcttgggg gattgagacc gactacggtt
     1681 atccacagaa tcaggggata acgca
//
