LOCUS       sequence-249763-        1753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Mitochondria Targeting Sequence as a Type 4a part to be used
            in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK4a_005
FEATURES             Location/Qualifiers
     source          1..1753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      173..761
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     662..681
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(980..1014)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1085..1744)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1659..1678
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
       61 atggtgaaag ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg
      121 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      181 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      241 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      301 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
      361 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      421 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      481 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      541 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      601 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      661 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      721 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      781 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
      841 ctgattctgt ggataaccgt agtcggtctc aatccctgag cctgcggcag agcatccggt
      901 tcttcaagcc cgccacccgg accctgtgca gcagccggta cctgctggtc gagtaactcg
      961 agtggctgag accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     1021 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
     1081 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     1141 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     1201 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     1261 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca
     1321 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     1381 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     1441 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     1501 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
     1561 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     1621 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     1681 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     1741 ccattttagc ttc
//
