LOCUS       sequence-249769-       12622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses gRNA in Aedes aegypti.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12622)
  AUTHORS   Li M, Yang T, Kandul NP, Bui M, Gamez S, Raban R, Bennett J, Sanchez
            C HM, Lanzaro GC, Schmidt H, Lee Y, Marshall JM, Akbari OS
  TITLE     Development of a confinable gene drive system in the human disease 
            vector Aedes aegypti.
  JOURNAL   Elife. 2020 Jan 21;9. pii: 51701. doi: 10.7554/eLife.51701.
  PUBMED    31960794
REFERENCE   2  (bases 1 to 12622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-01-21"; volume: "9. pii: 51701. doi"; pages: " 
            10.7554/eLife.51701"
COMMENT     Sequence Label: U6b
FEATURES             Location/Qualifiers
     source          1..12622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    207..328
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   996..1030
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(1211..1227)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1211..1227)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1224..1246)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1235..1251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1259..1265,1266..1283,1284..1289))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1304..1325
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1442..1459)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1613..2201)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1693..1712)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2372..3163,3164..3232))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2995..3014
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3233..3337)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3405..3423
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3461..3483)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3583..3602
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3796..3818
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3810..3827
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3811..3827
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   4531..4593
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      8614..8623
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    10172..10293
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             join(12035..12037,12038..12040,12041..12622,1..93)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
ORIGIN
        1 gactccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga gcagtacgag
       61 cgcaccgagg gccgccacca cctgttcctg taggactcta gatcataatc agccatacca
      121 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
      181 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
      241 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
      301 gtttgtccaa actcatcaat gtatcttaaa gcttctaggc cggccgatct cggatctgac
      361 aatgttcagt gcagagactc ggctacgcct cgtggacttt gaagttgacc aacaatgttt
      421 attcttacct ctaatagtcc tctgtggcaa ggtcaagatt ctgttagaag ccaatgaaga
      481 acctggttgt tcaataacat tttgttcgtc taatatttca ctaccgcttg acgttggctg
      541 cacttcatgt acctcatcta taaacgcttc ttctgtatcg ctctggacgt catcttcact
      601 tacgtgatct gatatttcac tgtcagaatc ctcaccaaca agctcgtcat cgctttgcag
      661 aagagcagag aggatatgct catcgtctaa agaactaccc attttattat atattagtca
      721 cgatatctat aacaagaaaa tatatatata ataagttatc acgtaagtag aacatgaaat
      781 aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata atcatgcgtc
      841 attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca ttgacaagca
      901 cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg acggattcgc
      961 gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta cgcagactat
     1021 ctttctaggg ttaaaaaaga tttgcgcttt actcgaccta aactttaaac acgtcataga
     1081 atcttcgttt gacaaaaacc acattgtggc caagctgtgt gacgcgacgc gcgctaaaga
     1141 atggcaaacc aagtcgcgcg agcgtcgact ctagaggatc cccgggtacc gagctcgaat
     1201 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     1261 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     1321 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     1381 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1441 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1501 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1561 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1621 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1681 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1741 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1801 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1861 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1921 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1981 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2041 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2101 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2161 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2221 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2281 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2341 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2401 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2461 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2521 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2581 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2641 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2701 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2761 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2821 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2881 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2941 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     3001 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3061 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3121 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3181 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3241 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3301 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3361 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3421 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     3481 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     3541 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     3601 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     3661 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     3721 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     3781 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctttgtt
     3841 taaaaatata acaaaattgt gatcccacaa aatgaagtgg ggcaaaatca aataattaat
     3901 agtgtccgta aacttgttgg tcttcaactt tttgaggaac acgttggacg gcaaatccgt
     3961 gactataaca caagttgatt taataatttt agccaacacg tcgggctgcg tgttttttgc
     4021 cgacgcgtct gtgtacacgt tgattaactg gtcgattaaa ctgttgaaat aatttaattt
     4081 ttggttcttc tttaaatctg tgatgaaatt ttttaaaata actttaaatt cttcattggt
     4141 aaaaaatgcc acgttttgca acttgtgagg gtctaatatg aggtcaaact cagtaggagt
     4201 tttatccaaa aaagaaaaca tgattacgtc tgtacacgaa cgcgtattaa cgcagagtgc
     4261 aaagtataag agggttaaaa aatatatttt acgcaccata tacgcatcgg gttgatatcg
     4321 ttaatatgga tcaatttgaa cagttgatta acgtgtctct gctcaagtct ttgatcaaaa
     4381 cgcaaatcga cgaaaatgtg tcggacaata tcaagtcgat gagcgaaaaa ctaaaaaggc
     4441 tagaatacga caatctcaca gacagcgttg agatatacgg tattcacgac agcaggctga
     4501 ataataaaaa aattagaaac tattatttaa ccctagaaag ataatcatat tgtgacgtac
     4561 gttaaagata atcatgcgta aaattgacgc atgtgtttta tcggtctgta tatcgaggtt
     4621 tatttattaa tttgaataga tattaagttt tattatattt acacttacat actaataata
     4681 aattcaacaa acaatttatt tatgtttatt tatttattaa aaaaaaacaa aaactcaaaa
     4741 tttcttctat aaagtaacaa aacttttaaa cattctctct tttacaaaaa taaacttatt
     4801 ttgtacttta aaaacagtca tgttgtatta taaaataagt aattagctta acttatacat
     4861 aatagaaaca aattatactt attagtcagt cagaaacaac tttggcacat atcaatatta
     4921 tgctctcgac aaataacttt tttgcatttt ttgcacgatg catttgcctt tcgccttatt
     4981 ttagaggggc agtaagtaca gtaagtacgt tttttcatta ctggctcttc agtactgtca
     5041 tctgatgtac caggcacttc atttggcaaa atattagaga tattatcgcg caaatatctc
     5101 ttcaaagtag gagcttctaa acgcttacgc ataaacgatg acgtcaggct catgtaaagg
     5161 tttctcataa attttttgcg actttgaacc ttttctccct tgctactgac attatggctg
     5221 tatataataa aagaatttat gcaggcaatg tttatcattc cgtacaataa tgccataggc
     5281 cacctattcg tcttcctact gcaggtcatc acagaacaca tttggtctag cgtgtccact
     5341 ccgcctttag tttgattata atacataacc atttgcggtt taccggtact ttcgttgata
     5401 gaagcatcct catcacaaga tgataataag tataccatct tagctggctt cggtttatat
     5461 gagacgagag taaggggtcc gtcaaaacaa aacatcgatg ttcccactgg cctggagcga
     5521 ctgtttttca gtacttccgg tatctcgcgt ttgtttgatc gcacggttcc cacaatggtt
     5581 aattcgagct cgcccggggt cctaggcctc acgccactcg tagaatgaac ccgattgaat
     5641 catttttaaa ccgaaaaaaa aatcctgccg gtgttctctg agttttctga ggattctgat
     5701 cgggatccca gaggaacctg ggaattgcta ttggattcct aacagactct tgagaattac
     5761 catcagagtt tcgtacattt ttaacaagat tttttgtgga atcatcacca aaccatgaaa
     5821 atttctagca ggcttttggg gttcctcagt gggatcatga aactccgtta catcgttttt
     5881 gctatgagat tacagaaaaa ataacttaag taaacagttt ttctgtaaaa gatatttcac
     5941 tcacttagct tttgaacgca gtggaatttt agaccattgt ttgcttgact ttttttttaa
     6001 tcataattta aatgttagag tgttagaaac cgtgttctta taaacaatat tctccttttc
     6061 ttcttccctg tcttgcgaaa caaacgatag agagaaactt atcagtgtac ctggcgtcta
     6121 agctgcgaat caaggaacta cgattttcca gccacttcct atgtaaatca atcgaagtat
     6181 cgtttagtac attacattta atacatttct tcttcatcta tgcatctttg acttttccaa
     6241 aatgaatatc ctatttcatt atacattttt tcttttactt ccagcgctac agcaccagca
     6301 gttatcagga tgtagatgaa ggtatcaaca gttcgttcgg cagcaatgac aaatccacgc
     6361 tgattcaggt ttggaaaccc aaaagttatg gcgccgtcaa gggacaaatt ccacagcacg
     6421 aacgactcac atacacatgg aaggagatcg atgtgtttgg agagacgcca ggtgatacca
     6481 aaaaggaacc cctgtgcagt agattgtgct gttgtttctc ccgccagaag aaagacttca
     6541 atccccgcaa acacctactg aagaatgtga ccgggatggc taagagtgga gagttactgg
     6601 ctgtgatggg tagttctggc gccggtaaaa caaccttgct gaacgctttg ctagctcatc
     6661 acaaacgaaa ctattataat cgtcttcagg ggaacggtag catcatatct gtcaccagtc
     6721 agtcggattc ctttctttcg gcataaatta tgctcgggtg acgagacgag ttgaaaatcc
     6781 cgtaaattaa aacatatacc acactcgccg tcctagacga cgacgacgac gacgattccc
     6841 aaggaattga acccttcatt tgcatttcac taccgctctg agagatgcaa agcgcgccag
     6901 acagtcgatc gaatctgcag accagagagt cacatcaaga atgttgacat tccaccagaa
     6961 gactctaatt attcttcgct cgactgggaa aactgttaac aactgctcga gtgtggtcgg
     7021 agcggctcac agtgcgtttt cccgtagaca aaaatcaaat ttcatggtat ttttcggcga
     7081 taaacagtaa gttaaaggga acccgaattt agtgctatat aatttaattc cactagagtt
     7141 tgtatccttt gatagatacg cgtatttcga cctcaactgc aaggccgtcg tgtactagac
     7201 ttgactaatc cagactggtc ttttagttat gacttctgtc cacatctcca tacattcaac
     7261 gcactgtgcg gctgtgctgt gcgactccgt cgagtcgacc aacatagttg aaacaaattg
     7321 aatatttaat tgatcgttat aggaatggtg ttagatgagt catcctttac agtaagcaca
     7381 tacagtatta taattgaaga tcgtcggcag ataggtgtgt agggtagagt atcagcaata
     7441 agttgggacg tttgactttt tgtaggtaga caaaaactaa actttttttc gcttctctat
     7501 gtgtgcccct gggtagcgtt ccgttccgat tggggtgcga acgaatgaaa tcgcccatcg
     7561 agttgatacg tccatccatc gctagaaccg cgttcgctgt agaagactat ataagagcag
     7621 aggcaagagt agtgaaatgg tgcgatcttg acccccgggt cttagagcta tgctggaaac
     7681 agcatagcaa gttaagataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
     7741 gtgctttttt tggtggttgt tcatcgattc ctaatattgc agtgaagcag tttgttgtgc
     7801 atggcgaaga taaggttagg gcgaggaatg ccttggaaat aagcggcact gggccgtatg
     7861 aataaaaagc gttgaacttt actacatgta gcatctactc aaaaacaaaa tccacaaagt
     7921 ttactacact tttggtatga ataaaaacct tttcgaacat gtagtaccta ctactttaag
     7981 aaaattctat tcaaagtaca gagtaatgct gcatgttaag aaaattccat tcagagtgac
     8041 tcttgaaatc aataaaatca tgcatatatg ccaaatttgt cacttattca cgtgacaaat
     8101 tatgtgaaaa tatgaatatt actacattcc cgctatgcat cgcgtaatcg atcatgaaat
     8161 ttatgttggt tatgtaactt tacagtattc cctttatgtg catgtgaatt ttataatgat
     8221 ttgacaacaa ggattttcct tagaattttc gaattttagc tagaaatctg tatcctatga
     8281 atgaatcttg agataacagc aattgtaaca ttataaagtt cctaacattt tcaagattcc
     8341 agtattgagt taattaattc gagctcgccc ggggatctaa ttcaattaga gactaattca
     8401 attagagcta attcaattag gatccaagct tatcgatttc gaaccctcga ccgccggagt
     8461 ataaatagag gcgcttcgtc tacggagcga caattcaatt caaacaagca aagtgaacac
     8521 gtcgctaagc gaaagctaag caaataaaca agcgcagctg aacaagctaa acaatcgggg
     8581 taccgctaga gtcgacggta cgatccaccg gtcgccacca tggtgagcaa gggcgaggag
     8641 gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag
     8701 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     8761 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg
     8821 tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc
     8881 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc
     8941 gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc
     9001 accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     9061 accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag
     9121 ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc
     9181 gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag
     9241 gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgggg
     9301 catggcaccg gcagcaccgg cagcggcagc tccggcaccg cctcctccga ggacaacaac
     9361 atggccgtta tcaaggaatt tatgcgcttc aaagttagga tggagggatc catgaacgga
     9421 catgagttcg agatcgaggg agagggcgag ggacgcccgt atgaaggcac acaaacagcc
     9481 aaactcaagg tcaccaaggg cggaccactg cccttcgcct gggatatcct gagtccccag
     9541 tttatgtacg gcagcaaggc ctacgttaag caccccgctg acataccgga ctacaaaaag
     9601 ctgtcctttc cggaaggctt caagtgggag cgcgtgatga atttcgaaga cggaggactg
     9661 gtcactgtga cccaagatag cagtttgcag gacggtacac tgatctataa ggttaaaatg
     9721 cgcggcacta actttccgcc agatggccca gtgatgcaga agaagaccat gggttgggag
     9781 gcatccaccg aacgtctgta ccctcgagac ggagtgctca agggcgagat ccatcaggcc
     9841 ctcaaactga aagatggtgg tcactacctg gtcgaattta agaccattta catggccaag
     9901 aagccggttc agctgcccgg atattattat gtggatacga aactggatat aacttcgcat
     9961 aacgaagact acaccattgt cgagcagtat gagcgcagcg aaggccgaca tcacctgttc
    10021 ctctacggca tggacgagct gtacaagtag gcggccgcga ctctagatca taatcagcca
    10081 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
    10141 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
    10201 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    10261 ttgtggtttg tccaaactca tcaatgtatc ttaaagcttg tttaaactca agatcgcacc
    10321 gacttcagta cgtgccctaa acggaatccc agtcaacgcg gaacagttgc gagcacgttg
    10381 cgcctatgtg caacaggacg atcttttcat tcccgcgctt acaactcgag aacacctcgt
    10441 attccacgca atgctgcgta tgggtaaaga tgtacccaag agtgtcaaga tgaatcgagt
    10501 caacgaggtg cttcaggagc tttccttagc gaaatgcgca gacacgatca ttggagcgcc
    10561 aggtcgaatg aaaggcctgt ccggaggtga acggaaacgg ttggcctttg catcggaaac
    10621 cctgaccgat cctcacctgt tgctttgtga tgaacccact tccgggttgg attcgttcat
    10681 ggctcacagt gttctacagg tgctgaaggg aatggccctc aaaggaaaga cgattatact
    10741 gaccatacat caaccttcgt cagaactgta ttgcttgttc gacaaaattc tcctagtggc
    10801 agaaggacgc gtggctttcc ttggatctcc ctatcaggct tcggaattct tttctcagtg
    10861 agtagacttg tgaaatcctg acgattttgc tttactagca gggcttgtgg tgtcttgcct
    10921 cttaaaacac cgaacatgaa acccaaaagc caaacgcaat aggaatcaga cgatgagagt
    10981 tcttcataga tctgaccaga catgtctcta agaatatttc taagactttt tttttggaaa
    11041 ttcatcgcga tcttgacatt acgcgtaatc cgcgtaatca tcgtattttt ccaaagttac
    11101 attcagatat tacggcagaa attctttagg agatttcatc aggagataac atattggtga
    11161 attacggatt cgttgtattt atcttcaaga atactacctg attttagttt agcaattact
    11221 tcagagattt catttagaat tcaagattca tgcagaaata ttctttcatt gatagaactt
    11281 ccttctatgc agaacatcta ccagaattca accagaaatt tctacttata tacctctaac
    11341 acggccaatt gcggccagac agtaaaataa tagtggcctt agtaactaaa atgatcaaaa
    11401 ttgtgacccg gtaaagtgat ttaataaaac agtttttagt ccaaacaata ttaggggtca
    11461 tccaaaaacg acttcaatca ttaggggcgc gcccatactc ggtggcctcc ccaccaccaa
    11521 cttttttgca ctgcaaaaaa acacgctttt gcacgcgggc ccatacatag tacaaactct
    11581 acgtttcgta gactatttta cataaatagt ctacaccgtt gtatacgctc caaatacact
    11641 accacacatt gaaccttttt gcagtgcaaa aaagtacgtg tcggcagtca cgtaggccgg
    11701 ccttatcggg tcgcgtcctg tcacgtacga atcacattat cggaccggac gagtgttgtc
    11761 ttatcgtgac aggacgccag cttcctgtgt tgctaaccgc agccggacgc aactccttat
    11821 cggaacagga cgcgcctcca tatcagccgc gcgttatctc atgcgcgtga ccggacacga
    11881 ggcgcccgtc ccgcttatcg cgcctataaa tacagcccgc aacgatctgg taaacacagt
    11941 tgaacagcat ctgttacagc gacacaacat gagccggtcc aacaacgcca acgcgcccac
    12001 gccatccaac cgccgccgca acctgtctct ggtgatggtg cgctcctcca agaacgtcat
    12061 caaggagttc atgcgcttca aggtgcgcat ggagggcacc gtgaacggcc acgagttcga
    12121 gatcgagggc gagggcgagg gccgccccta cgagggccac aacaccgtga agctgaaggt
    12181 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tccagtacgg
    12241 ctccaaggtg tacgtgaagc accccgccga catccccgac tacaagaagc tgtccttccc
    12301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
    12361 ccaggactcc tccctgcagg acggctgctt catctacaag gtgaagttca tcggcgtgaa
    12421 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
    12481 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc cacaaggccc tgaagctgaa
    12541 ggacggcggc cactacctgg tggagttcaa gtccatctac atggccaaga agcccgtgca
    12601 gctgcccggc tactactacg tg
//
