LOCUS       sequence-249784-        1942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Gibberellin Insensitive as a Type 4a part to be used in the 
            MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK4a_023
FEATURES             Location/Qualifiers
     source          1..1942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      113..701
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     602..621
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             825..842
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(1109..1143)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1214..1873)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1788..1807
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 atggtgaaag ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg
       61 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      121 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      181 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      241 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
      301 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      361 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      421 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      481 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      541 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      601 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      661 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      721 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
      781 ctgattctgt ggataaccgt agtcggtctc aatccaagag agatcatcat catcatcatc
      841 atcaagataa gaagactatg atgatgaatg aagaagacga cggtaacggc atggatgagc
      901 ttctagctgt tcttggttac aaggttaggt catccgaaat ggctgatgtt gctcagaaac
      961 tcgagcagct tgaagttatg atgtctaatg ttcaagaaga cgatctttct caactcgcta
     1021 ctgagactgt tcactataat ccggcggagc tttacacgtg gcttgattct atgctcaccg
     1081 accttaatta atggctgaga ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct
     1141 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     1201 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     1261 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     1321 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     1381 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaaa
     1441 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     1501 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     1561 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     1621 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     1681 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     1741 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     1801 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     1861 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     1921 ccggtagtga tcttatttca tt
//
