LOCUS       sequence-249785-        2569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Abscisic Acid Insensitive as a Type 4a part to be used in 
            the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK4a_024
FEATURES             Location/Qualifiers
     source          1..2569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      113..701
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     602..621
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1736..1770)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1841..2500)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2415..2434
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 atggtgaaag ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg
       61 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      121 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      181 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      241 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
      301 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      361 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      421 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      481 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      541 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      601 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      661 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      721 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
      781 ctgattctgt ggataaccgt agtcggtctc aatccacgcg tgtgcctttg tatggtttta
      841 cttcgatttg tggaagaaga cctgagatgg aagctgctgt ttcgactata ccaagattcc
      901 ttcaatcttc ctctggttcg atgttagatg gtcggtttga tcctcaatcc gccgctcatt
      961 tcttcggtgt ttacgacggc catggcggtt ctcaggtagc gaactattgt agagagagga
     1021 tgcatttggc tttggcggag gagatagcta aggagaaacc gatgctctgc gatggtgata
     1081 cgtggctgga gaagtggaag aaagctcttt tcaactcgtt cctgagagtt gactcggaga
     1141 ttgagtcagt tgcgccggaa acggttgggt caacgtcggt ggttgccgtt gttttcccat
     1201 ctcacatctt cgtcgctaac tgcggtgact ctagagccgt tctttgccgc ggcaaaactg
     1261 cacttccatt atccgttgac cataaaccgg atagagaaga tgaagctgcg aggattgaag
     1321 ccgcaggagg gaaagtgatt cagtggaatg gagctcgtgt tttcggtgtt ctcgccatgt
     1381 cgagatccat tggcgataga tacttgaaac catccatcat tcctgatccg gaagtgacgg
     1441 ctgtgaagag agtaaaagaa gatgattgtc tgattttggc gagtgacggg gtttgggatg
     1501 taatgacgga tgaagaagcg tgtgagatgg caaggaagcg gattctcttg tggcacaaga
     1561 aaaacgcggt ggctggggat gcatcgttgc tcgcggatga gcggagaaag gaagggaaag
     1621 atcctgcggc gatgtccgcg gctgagtatt tgtcaaagct ggcgatacag agaggaagca
     1681 aagacaacat aagtgtggtg gtggttgatt tgaagtaatg gctgagacca gaccaataaa
     1741 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     1801 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     1861 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     1921 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     1981 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     2041 tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac cctttaggga
     2101 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     2161 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     2221 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     2281 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     2341 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     2401 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     2461 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     2521 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatt
//
