LOCUS       sequence-24979-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Zhang Y, Nash L, Fisher AL.
  TITLE     A simplified, robust, and streamlined procedure for the production 
            of C. elegans transgenes via recombineering
  JOURNAL   BMC Dev Biol. 2008 Dec 30;8(1):119.
  PUBMED    19116030
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2008-12-30"; volume: "8(1)"; pages: "119"
COMMENT     Sequence Label: pMOD4 galK-GT
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             199..222
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        271..318
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 gcgtgtctct tatacacatc ntcaaccctg naagctcttg ttggctagtg cgtagtcgtt
       61 ggcaagcttg catgcctgca gatggcaggc cttgcgcaac acgatgaagc cgtggacaac
      121 aaattcaaca aaaccggtat ggaaaagaga agatggaaaa agaatttcat agccgtctca
      181 gcagccaact ctagaatgga ttacaaggac gatgacgata agatgagtaa aggagaagaa
      241 cttttcactg gagttgtccc aagaattcct gttgacaatt aatcatcggc atagtatatc
      301 ggcatagtat aatacgacaa ggtgaggaac taaacccagg aggcagatca tgagtctgaa
      361 agaaaaaaca caatctctgt ttgccaacgc atttggctac cctgccactc acaccattca
      421 ggcgcctggc cgcgtgaatt tgattggtga acacaccgac tacaacgacg gtttcgttct
      481 gccctgcgcg attgattatc aaaccgtgat cagttgtgca ccacgcgatg accgtaaagt
      541 tcgcgtgatg gcagccgatt atgaaaatca gctcgacgag ttttccctcg atgcgcccat
      601 tgtcnnnaca tgaaaactat caatgggcta actacnttcn nngncgnnnn nnnncntctg
      661 caactgcgta acaacagctt cngcggcgtg gacatggtga tcagcggcaa tgtgccgcaa
      721 gggtgccggg ttaagttctn ccgctttcac tgggaagtcg cggttcggaa cc
//
