LOCUS       sequence-249790-        2713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ha-tagged and nuclear localized Abscisic Acid Insensitive as
            a Type 4a part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK4a_026
FEATURES             Location/Qualifiers
     source          1..2713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      113..701
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     602..621
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             819..845
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             825..851
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             864..884
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             891..911
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(1880..1914)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1985..2644)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2559..2578
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 atggtgaaag ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg
       61 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      121 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      181 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      241 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
      301 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      361 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      421 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      481 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      541 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      601 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      661 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      721 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
      781 ctgattctgt ggataaccgt agtcggtctc aatccgccta tccctatgac gtgcccgatt
      841 atgccagcct gggcagcggc tcccccaaga aaaaacgcaa ggtggaagat cctaagaaaa
      901 agcggaaagt ggacggcatt ggtagtggga gcaacggagg tggaggctct ggacctagga
      961 cgcgtgtgcc tttgtatggt tttacttcga tttgtggaag aagacctgag atggaagctg
     1021 ctgtttcgac tataccaaga ttccttcaat cttcctctgg ttcgatgtta gatggtcggt
     1081 ttgatcctca atccgccgct catttcttcg gtgtttacga cggccatggc ggttctcagg
     1141 tagcgaacta ttgtagagag aggatgcatt tggctttggc ggaggagata gctaaggaga
     1201 aaccgatgct ctgcgatggt gatacgtggc tggagaagtg gaagaaagct cttttcaact
     1261 cgttcctgag agttgactcg gagattgagt cagttgcgcc ggaaacggtt gggtcaacgt
     1321 cggtggttgc cgttgttttc ccatctcaca tcttcgtcgc taactgcggt gactctagag
     1381 ccgttctttg ccgcggcaaa actgcacttc cattatccgt tgaccataaa ccggatagag
     1441 aagatgaagc tgcgaggatt gaagccgcag gagggaaagt gattcagtgg aatggagctc
     1501 gtgttttcgg tgttctcgcc atgtcgagat ccattggcga tagatacttg aaaccatcca
     1561 tcattcctga tccggaagtg acggctgtga agagagtaaa agaagatgat tgtctgattt
     1621 tggcgagtga cggggtttgg gatgtaatga cggatgaaga agcgtgtgag atggcaagga
     1681 agcggattct cttgtggcac aagaaaaacg cggtggctgg ggatgcatcg ttgctcgcgg
     1741 atgagcggag aaaggaaggg aaagatcctg cggcgatgtc cgcggctgag tatttgtcaa
     1801 agctggcgat acagagagga agcaaagaca acataagtgt ggtggtggtt gatttgaagt
     1861 aatggctgag accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     1921 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
     1981 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     2041 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     2101 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     2161 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca
     2221 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     2281 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     2341 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     2401 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
     2461 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     2521 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     2581 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     2641 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     2701 atcttatttc att
//
