LOCUS       sequence-249793-        1796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SV40 polyadenylation signal as a Type 4b part to be used in 
            the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK4b_002
FEATURES             Location/Qualifiers
     source          1..1796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(38..57)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     92..111
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      complement(161..195)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(266..925)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     840..859
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1107..1695
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1596..1615
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat ggcgatccag acatgataag atacattgat gagtttggac aaaccacaac
       61 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      121 aaccattata agctgcaata aacgctgtga gaccagacca ataaaaaacg cccggcggca
      181 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
      241 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
      301 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
      361 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
      421 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
      481 gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
      541 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
      601 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
      661 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
      721 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
      781 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
      841 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
      901 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
      961 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     1021 gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1081 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1141 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1201 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1261 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1321 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1381 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1441 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1501 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1561 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1621 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1681 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1741 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtag
//
