LOCUS       sequence-249795-        2223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes rabbit beta-globin polyadenylation signal as a Type 4b part 
            to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK4b_003
FEATURES             Location/Qualifiers
     source          1..2223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     28..47
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(323..342)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    388..443
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(442..461)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     terminator      complement(816..850)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(921..1580)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1495..1514
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1762..2223,1..127)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
       61 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      121 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      181 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtagtc ggtctcatgg
      241 cattcactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg gccaatgccc
      301 tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg gacatcatga
      361 agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt gcaatagtgt
      421 gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttaaaac
      481 atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg gctgccatga
      541 acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct gtccattcct
      601 tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt tttgtgttat
      661 ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga tttttcctcc
      721 tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc ctcgacctgc
      781 agcccaagct tggcgtaagc tgtgagacca gaccaataaa aaacgcccgg cggcaaccga
      841 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
      901 caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat
      961 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
     1021 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
     1081 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
     1141 gctgaaacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
     1201 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
     1261 ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca
     1321 ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc
     1381 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg
     1441 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact
     1501 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat
     1561 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa
     1621 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg
     1681 atcaatcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1741 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1801 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1861 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1921 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1981 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     2041 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2101 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2161 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     2221 aac
//
