LOCUS       sequence-249796-        1703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes spacer sequence as a Type 4b part to be used in the MTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK4b_004
FEATURES             Location/Qualifiers
     source          1..1703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     203..222
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(471..505)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(576..1235)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1150..1169
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1417..1703,1..302)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
       61 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      121 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      181 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      241 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      301 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      361 tgttctttcc tgcgttatcc cctgattctg tggataaccg tagtcggtct catggcacta
      421 gagatctatg tgaggatcct aactcgagat cgagctgtga gaccagacca ataaaaaacg
      481 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
      541 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg
      601 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
      661 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
      721 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
      781 ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag
      841 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
      901 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
      961 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
     1021 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     1081 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     1141 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     1201 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     1261 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     1321 cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1381 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1441 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1501 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1561 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1621 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1681 taccgggttg gactcaagac gat
//
