LOCUS       sequence-249799-        2448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ConRE' and the chicken hypersensitivity site 4 insulator as 
            a Type 5 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK5_008
FEATURES             Location/Qualifiers
     source          1..2448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      174..762
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     663..682
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1676..1710)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1781..2440)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2355..2374
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
       61 tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt
      121 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      181 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      241 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      301 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
      361 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
      421 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      481 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      541 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      601 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      661 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      721 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      781 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      841 cctgattctg tggataaccg tagtcggtct cagctggaaa tctgctcgtc agtggtgctc
      901 acactgacga atcatgtaca gatcataccg atgactgcct ggcgactcac aactaagcaa
      961 gacagccgga accagcgccg gcgaacacca ctgcatatat ggcatatcac aacagtccac
     1021 gtctcaagca gttacagaga tgttacgaac cactagtgca ctgcagatcg aattcgagtt
     1081 ggcgcgcctg tcattctaaa tctctctttc agcctaaagc tttttccccg tatcccccca
     1141 ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc
     1201 ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg
     1261 accggagcgg agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac
     1321 atccctgggg gctttgggag ggggctgtcc ccgtgagctc ccaggcgcgc ctgtcattct
     1381 aaatctctct ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa
     1441 agagcagcga gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct
     1501 gtccccgcac gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg
     1561 ggcggctcgc tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg
     1621 gggggggctg tccccgtgag ctcaataaaa gagcccacta catgagacca gaccaataaa
     1681 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     1741 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     1801 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     1861 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     1921 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     1981 tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac cctttaggga
     2041 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     2101 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     2161 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     2221 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     2281 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     2341 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     2401 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagctt
//
