LOCUS       sequence-249802-        1835 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ConR8 as a Type 5 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK5_011
FEATURES             Location/Qualifiers
     source          1..1835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(200..234)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(305..964)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     879..898
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1146..1734
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1635..1654
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcag ctgtttatga gacgggaatg tacggaacct cgatgccatt gcatctgaac
       61 gcctggcgat cagacgacac tagagtcacg acgataccag gtctgtcata tgagcggaac
      121 ccatgccaga ttgacccaac tccatgaaac tacgatgcag agactggact agtgcactgc
      181 agtacatgag accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
      241 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat
      301 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
      361 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
      421 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
      481 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca
      541 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
      601 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
      661 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
      721 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa
      781 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
      841 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
      901 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
      961 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     1021 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa
     1081 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1141 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1201 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1261 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1321 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1381 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     1441 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     1501 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     1561 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     1621 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     1681 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     1741 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     1801 cctgcgttat cccctgattc tgtggataac cgtag
//
