LOCUS       sequence-249803-        2024 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes ConRE' and the human A1 insulator as a Type 5 part to be 
            used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK5_012
FEATURES             Location/Qualifiers
     source          1..2024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      174..762
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     663..682
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1252..1286)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1357..2016)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1931..1950
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
       61 tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt
      121 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
      181 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      241 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      301 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
      361 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
      421 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      481 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      541 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      601 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      661 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      721 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      781 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      841 cctgattctg tggataaccg tagtcggtct cagctggaaa tctgctcgtc agtggtgctc
      901 acactgacga atcatgtaca gatcataccg atgactgcct ggcgactcac aactaagcaa
      961 gacagccgga accagcgccg gcgaacacca ctgcatatat ggcatatcac aacagtccac
     1021 gtctcaagca gttacagaga tgttacgaac cctgaacagg ttgactatta attgtgtctg
     1081 cttgatgtgg acaccaggtg gcgctggaca tcagatttgg agaggcagtt gtctagggaa
     1141 ccgggctctg tgccagcgca ggaggcaggc tggctctcct attccaggga tgctcatcca
     1201 ggaaggaaag gttgcatgct ggacacacta accttacatg agaccagacc aataaaaaac
     1261 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     1321 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
     1381 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     1441 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     1501 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     1561 actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt tagggaaata
     1621 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     1681 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     1741 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
     1801 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     1861 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     1921 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     1981 atcaacggtg gtatatccag tgattttttt ctccatttta gctt
//
