LOCUS       sequence-24981-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Zhang Y, Nash L, Fisher AL.
  TITLE     A simplified, robust, and streamlined procedure for the production 
            of C. elegans transgenes via recombineering
  JOURNAL   BMC Dev Biol. 2008 Dec 30;8(1):119.
  PUBMED    19116030
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2008-12-30"; volume: "8(1)"; pages: "119"
COMMENT     Sequence Label: ploxP unc-119
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(205..309)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     377..395
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(433..455)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     555..574
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 taggcnacat tggaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
       61 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
      121 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
      181 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
      241 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
      301 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
      361 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
      421 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
      481 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
      541 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
      601 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc ccattcaggc
      661 tgctgnactg ttgggaaagg cgatcgnggc ggcctntncc ntnttacnaa gctgtcntta
      721 tacacatctc accatcatcg atgantttcn cgggggtctc catatggccn aatgaaactc
      781 ctaanngngg cnaaca
//
