LOCUS       sequence-249816-        1700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the inverted attB phiC31 transposon recognition site 
            cassette as a type 6 part to be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK6_011
FEATURES             Location/Qualifiers
     source          1..1700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     111..130
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      378..966
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     867..886
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1081..1114
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     terminator      complement(1132..1166)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(1237..1700,1..196))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
       61 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
      121 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
      181 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
      241 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca
      301 atcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
      361 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
      421 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
      481 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
      541 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
      601 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
      661 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
      721 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
      781 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      841 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      901 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      961 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     1021 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtagtcg gtctcataca
     1081 cgcgcccggg gagcccaagg gcacgccctg gcacgagttg agaccagacc aataaaaaac
     1141 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     1201 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcatc
     1261 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     1321 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     1381 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     1441 actcacccag ggattggctg aaacgaaaaa catattctca ataaaccctt tagggaaata
     1501 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     1561 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     1621 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
     1681 ttccggatga gcattcatca
//
