LOCUS       sequence-24982-e         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Zhang Y, Nash L, Fisher AL.
  TITLE     A simplified, robust, and streamlined procedure for the production 
            of C. elegans transgenes via recombineering
  JOURNAL   BMC Dev Biol. 2008 Dec 30;8(1):119.
  PUBMED    19116030
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2008-12-30"; volume: "8(1)"; pages: "119"
COMMENT     Sequence Label: ploxP unc-119
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..35
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
ORIGIN
        1 cgattcatta agacagctgt ctcttataca catctcaacc ctgaagctct tgttggctag
       61 tgcgtagtcg ttggcaagct tgcatgcctg caggtcgact ctagagaatt ttaacaatac
      121 ttcaaatttg ttaagccgaa cataacaaaa aatgaataaa aaacggaaag tgattgaccc
      181 gtatttgaag aagaagacaa atgagtgggg gcgacaatgg gggaaaaaga gacgatgaaa
      241 gtgaagtgga attaaatgtc ggaaaatttt gtggaaaaag aataatgaaa tatacatata
      301 atatcagtat cttcagcttt tcctcctgct tcacaatctc ggcggcagat ttatgtaaat
      361 gatatctcca ttcaaatggt attcaaagaa gaagaagaag aaggcaattg ctgataatga
      421 gataaaagga ggcttttcct actgataaca attggtcggg aattcatgat tagtggaaga
      481 aaagggtggt gttttggatg aagaagatgt tgggaatgcc aatgatcgga tttttgatgc
      541 aagcagttat cttttgcaac atgattagtc atttaaatca gggttggcga caaacaattt
      601 tttttccggc aaatcggcaa atccgttagn tngccggaat tggtaacttn ccggcnaatc
      661 gacnaatggn ncgnnnnnnn natgntcngc aatcggcaaa tcggcnnttg tcggaattga
      721 aatttncga
//
