LOCUS       sequence-249821-        2120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes the 5' 500 bp hRosa26 locus homology arm as a type 7 part to
            be used in the MTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Fonseca JP, Bonny AR, Kumar GR, Ng AH, Town J, Wu QC, Aslankoohi E, 
            Chen SY, Dods G, Harrigan P, Osimiri LC, Kistler AL, El-Samad H
  TITLE     A Toolkit for Rapid Modular Construction of Biological Circuits in 
            Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2019 Nov 15;8(11):2593-2606. doi: 
            10.1021/acssynbio.9b00322. Epub 2019 Nov 5.
  PUBMED    31686495
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00322"; journalName: "ACS Synth Biol"; date: 
            "2019-11-15- 15"; volume: "8"; issue: "11"; pages: "2593-2606"
COMMENT     Sequence Label: MTK7_002
FEATURES             Location/Qualifiers
     source          1..2120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      77..665
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     566..585
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1217..1233
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(1251..1285)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1356..2015)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1930..1949
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg tctcagagtg
      781 gaggcagcag agaactccca taaaggtatt gcggcactcc cctccccctg cccagaaggg
      841 tgcggccttc tctccacctc ctccaccgca gctccctcag gattgcagct cgcgccggtt
      901 tttggagaac aagcgcctcc cacccacaaa ccagccggac cgacccccgc tcctccccca
      961 cccccacgag tgcctgtagc aggtcgggct tgtctcgccc ttcaggcggt gggaacccag
     1021 ggcggagcct cggccgccgc catccagaag tctcggccgg cagcccgacc cctcctccag
     1081 cgcgcgcttc atgccacgtt gcgcaggggc gcggggacag acactgcggc gctcggcgtc
     1141 ggggaggacc gtaccaacgc ccgcctcccc tccacccccg cgccccgcgc agtggtttcg
     1201 ctcatgtgag actcgagtaa aacgacggcc agtccgatga gaccagacca ataaaaaacg
     1261 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     1321 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg
     1381 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
     1441 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
     1501 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     1561 ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag
     1621 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     1681 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
     1741 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
     1801 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     1861 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     1921 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     1981 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     2041 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     2101 cctcttacgt gcccgatcaa
//
